Total phenolic contents, in vitro antioxidant activity, enzymes inhibition and antiinflammatory effect of the selective extracts from the Algerian Lavandula multifida
ABSTRACT
The present research aimed to carry out the phytochemical analysis, in vitro antioxidant activity, enzymes inhibition and anti-inflammatory effect of the selective extracts of the Algerian L. multifida from two regions; Msila region and Constantine region. The total phenolic and flavonoids contents of crude extract (CE) and its solvent partition fractions: dichloromethane (DME), ethyl acetate (EAE) and n-butanol (BUE) were determined spectrophotometriclly. The antioxidant activity of extracts was achieved by the use of seven methods and the enzyme inhibitory activity of extracts was evaluated against α-amylase and butyrylcholinesterase. Moreover, the in vivo anti-inflammatory activity of the aqueous extract of L. multifida from Constantine region was evaluated using paw edema model. The M.EAE present the highest TPC and TFC, which were about 462.23±11.74µg GAE/mg extract and 125.90±0.16µg QE/mg extract, respectively. In addition, the M.EAE exhibited an excellent antioxidant activity, as it has a great ability to scavenging the DPPH, ABTS and galvinoxyl free radicals, as well as reducing power and metal chelating. However, the M.CE and C.CE showed the best inhibitory activity of the α-amylase butyrylcholinesterase (IC50=64.17±1.81µg/ ml and 83.55±1.97µg/ml, respectively. The preliminary investigation reveals that the EAE has a good bio-pharmacological activity, which it possesses an interesting potential for pharmaceutical/nutraceutical applications.
Keywords: Antioxidant activity, anti-inflammatory effect, enzymes inhibition, Lavandula.
ACTA PHARMACEUTICA SCIENCIA Dergisi 60/1. Sayısı İçin Tıklayınız.