Course Supervisor: Prof. Dr Esra Çağavi,
Course LaboAssistants: Laboratory supervisor from Research Institute for Health Sciences and Technologies (SABITA),
Aim
The aim of the “Laboratory Rotation-I” course is for Ph.D. level students to explain the advanced technical approaches in scientific research, the basic laboratory equipment usage instructions, and laboratory application that will be incorporated to the thesis work by understanding the scope of different laboratory disciplines. By rotating in different labs every week, students are aimed to explain laboratory safety and instructions and practice advanced molecular biology techniques, proteomics and genomics applications, flow cytometry and analysis, histology applications, microscopy imaging systems and image analysis, cell culture and experimental animal laboratories.
Course Content
This course contains; Advanced Molecular Biology Techniques-1, Lab-1,Advanced Molecular Biology Techniques-2, Lab-2,Electrophysiology laboratory,Advanced Molecular Biology Techniques-3, Lab-3,Cell Culture applications,Fluorescence-activated Cell Sorting (FACS),Lab notebook evaluation,Immunohistochemistry,Medicinal Chemistry and drup applicaitons,Genomics and Proteomics Methodology,Microscopy Imaging and analysis,Medical Research Centre (MEDITAM) and experimental animal models-I,Medical Research Centre (MEDITAM) and experimental animal models-II,Final.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Explain the advanced experimental methods and analysis approaches in molecular miology, proteomics, genomics, immunohistochemistry, imaging systems and analysis, cell culture and experimental animal laboratories.
14, 17, 2, 9
A, E
Incorporate, do research and apply new research techniques with the thesis subject.
14, 17, 2, 9
A, E
Explain and provide solutions to various different experimental techniques.
14, 17, 2, 9
A, E
Define the principles of basic research laboratory equipments and can operate these devices.
14, 17, 2, 9
A, E
Explains the advanced data analysis approaches of scientific data.
14, 17, 2, 9
A, E
Explain and evaluate the workplace and biological safety instructions in the laboratory.
14, 17, 2, 9
A, E
Teaching Methods:
14: Self Study Method, 17: Experimental Technique, 2: Project Based Learning Model, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, E: Homework
Course Outline
Order
Subjects
Preliminary Work
1
Advanced Molecular Biology Techniques-1, Lab-1
Self-study
2
Advanced Molecular Biology Techniques-2, Lab-2
Self-study
3
Electrophysiology laboratory
Self-study
4
Advanced Molecular Biology Techniques-3, Lab-3
Self-study
5
Cell Culture applications
Self-study
6
Fluorescence-activated Cell Sorting (FACS)
Self-study
7
Lab notebook evaluation
Self-study and lecture notes
8
Immunohistochemistry
Self-study
9
Medicinal Chemistry and drup applicaitons
Self-study
10
Genomics and Proteomics Methodology
Self-study
11
Microscopy Imaging and analysis
Self-study
12
Medical Research Centre (MEDITAM) and experimental animal models-I
Self-study
13
Medical Research Centre (MEDITAM) and experimental animal models-II
Self-study
14
Final
Self-study and lecture notes
Resources
Laboratory Biosafety Manuel(Third edition) 1. Containment of biohazard 2. Laboratoaries - standards 3. Laboratory infection - prevention and control 4. Manuals I. Title . ISBN 92 4 154650 6 (LC/NLM classification: QY 25) WHO/ CDS/ CSR/ LYO2004. 11
Internet database
Course Contribution to Program Qualifications
Course Contribution to Program Qualifications
No
Program Qualification
Contribution Level
1
2
3
4
5
1
Has the essential knowledge about the structure and functioning of the nervous system.
X
2
Knows the essential scientific questions and current research topics in neuroscience and produces hypotheses about them.
X
3
Have knowledge and interpretation skills about neurodegenerative processes.
X
4
Know the principles of technologies for the measurement of molecular and cellular level parameters.
X
5
Defines the methods used in neuroscience researches. In own research area uses the required techniques without assistance, develops new methods and techniques. Designs research projects and submits them as proposals. Presents the research findings in oral or written forms and in scientific articles.
X
6
Uses the communication and computer technology effectively in theoretical and practical studies.
X
7
Able to present theoretical or research data orally or written in Turkish as well in English languages.
X
8
Adheres to ethical values and behaves according to dynamics of social responsibility.
X
9
While focusing on the basic research behaves in accordance with the principle that neuroscience research should eventually aim clinical implications and benefit.
X
10
Designs and conducts scientific research, independently and within a team. Manages a research lab and establishes new ones.
X
11
Evaluates all new information regarding the field and associate them based on available knowledge.
X
Assessment Methods
Contribution Level
Absolute Evaluation
Rate of Midterm Exam to Success
50
Rate of Final Exam to Success
50
Total
100
ECTS / Workload Table
Activities
Number of
Duration(Hour)
Total Workload(Hour)
Course Hours
14
3
42
Guided Problem Solving
14
10
140
Resolution of Homework Problems and Submission as a Report
0
0
0
Term Project
0
0
0
Presentation of Project / Seminar
0
0
0
Quiz
0
0
0
Midterm Exam
0
0
0
General Exam
0
0
0
Performance Task, Maintenance Plan
0
0
0
Total Workload(Hour)
182
Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(182/30)
6
ECTS of the course: 30 hours of work is counted as 1 ECTS credit.
Course Supervisor: Prof. Dr Esra Çağavi,
Course LaboAssistants: Laboratory supervisor from Research Institute for Health Sciences and Technologies (SABITA),
Aim
The aim of the “Laboratory Rotation-I” course is for Ph.D. level students to explain the advanced technical approaches in scientific research, the basic laboratory equipment usage instructions, and laboratory application that will be incorporated to the thesis work by understanding the scope of different laboratory disciplines. By rotating in different labs every week, students are aimed to explain laboratory safety and instructions and practice advanced molecular biology techniques, proteomics and genomics applications, flow cytometry and analysis, histology applications, microscopy imaging systems and image analysis, cell culture and experimental animal laboratories.
Course Content
This course contains; Advanced Molecular Biology Techniques-1, Lab-1,Advanced Molecular Biology Techniques-2, Lab-2,Electrophysiology laboratory,Advanced Molecular Biology Techniques-3, Lab-3,Cell Culture applications,Fluorescence-activated Cell Sorting (FACS),Lab notebook evaluation,Immunohistochemistry,Medicinal Chemistry and drup applicaitons,Genomics and Proteomics Methodology,Microscopy Imaging and analysis,Medical Research Centre (MEDITAM) and experimental animal models-I,Medical Research Centre (MEDITAM) and experimental animal models-II,Final.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Explain the advanced experimental methods and analysis approaches in molecular miology, proteomics, genomics, immunohistochemistry, imaging systems and analysis, cell culture and experimental animal laboratories.
14, 17, 2, 9
A, E
Incorporate, do research and apply new research techniques with the thesis subject.
14, 17, 2, 9
A, E
Explain and provide solutions to various different experimental techniques.
14, 17, 2, 9
A, E
Define the principles of basic research laboratory equipments and can operate these devices.
14, 17, 2, 9
A, E
Explains the advanced data analysis approaches of scientific data.
14, 17, 2, 9
A, E
Explain and evaluate the workplace and biological safety instructions in the laboratory.
14, 17, 2, 9
A, E
Teaching Methods:
14: Self Study Method, 17: Experimental Technique, 2: Project Based Learning Model, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, E: Homework
Course Outline
Order
Subjects
Preliminary Work
1
Advanced Molecular Biology Techniques-1, Lab-1
Self-study
2
Advanced Molecular Biology Techniques-2, Lab-2
Self-study
3
Electrophysiology laboratory
Self-study
4
Advanced Molecular Biology Techniques-3, Lab-3
Self-study
5
Cell Culture applications
Self-study
6
Fluorescence-activated Cell Sorting (FACS)
Self-study
7
Lab notebook evaluation
Self-study and lecture notes
8
Immunohistochemistry
Self-study
9
Medicinal Chemistry and drup applicaitons
Self-study
10
Genomics and Proteomics Methodology
Self-study
11
Microscopy Imaging and analysis
Self-study
12
Medical Research Centre (MEDITAM) and experimental animal models-I
Self-study
13
Medical Research Centre (MEDITAM) and experimental animal models-II
Self-study
14
Final
Self-study and lecture notes
Resources
Laboratory Biosafety Manuel(Third edition) 1. Containment of biohazard 2. Laboratoaries - standards 3. Laboratory infection - prevention and control 4. Manuals I. Title . ISBN 92 4 154650 6 (LC/NLM classification: QY 25) WHO/ CDS/ CSR/ LYO2004. 11
Internet database
Course Contribution to Program Qualifications
Course Contribution to Program Qualifications
No
Program Qualification
Contribution Level
1
2
3
4
5
1
Has the essential knowledge about the structure and functioning of the nervous system.
X
2
Knows the essential scientific questions and current research topics in neuroscience and produces hypotheses about them.
X
3
Have knowledge and interpretation skills about neurodegenerative processes.
X
4
Know the principles of technologies for the measurement of molecular and cellular level parameters.
X
5
Defines the methods used in neuroscience researches. In own research area uses the required techniques without assistance, develops new methods and techniques. Designs research projects and submits them as proposals. Presents the research findings in oral or written forms and in scientific articles.
X
6
Uses the communication and computer technology effectively in theoretical and practical studies.
X
7
Able to present theoretical or research data orally or written in Turkish as well in English languages.
X
8
Adheres to ethical values and behaves according to dynamics of social responsibility.
X
9
While focusing on the basic research behaves in accordance with the principle that neuroscience research should eventually aim clinical implications and benefit.
X
10
Designs and conducts scientific research, independently and within a team. Manages a research lab and establishes new ones.
X
11
Evaluates all new information regarding the field and associate them based on available knowledge.
