To explain the molecular biology techniques used in the detection and identification of microorganisms in samples and to determine antimicrobial resistance and to apply basic techniques.
Course Content
This course contains; Access to microorganism genetic information (websites, programs and usage application),Methods used in nucleic acid isolation,Polymerase chain reaction (PCR),Quantitative polymerase chain reaction (qPCR),Methods used in RNA detection; RT-PCR, RT-qPCR,Dijital PCR,Sanger sequencing method,Next generation sequencing methods - I,Next generation sequencing methods - II,Microbial whole genome sequencing: application in clinical microbiology and public health,Methods used in microorganism genotyping,Current methods in molecular microbiology laboratory,Technical problems and solution suggestions encountered in the methods used in molecular biology,Problems and solution suggestions regarding specificity, sensitivity and interpretation of results in molecular microbiology tests.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Explains molecular methods used in the field of microbiology and applies basic methods.
10, 16, 17, 9
A
Evaluates the problems encountered in molecular biology tests and offers solutions.
10, 16, 17, 9
A
Lists the sources of access to genetic information and the computer programs used.
Access to microorganism genetic information (websites, programs and usage application)
2
Methods used in nucleic acid isolation
3
Polymerase chain reaction (PCR)
4
Quantitative polymerase chain reaction (qPCR)
5
Methods used in RNA detection; RT-PCR, RT-qPCR
6
Dijital PCR
7
Sanger sequencing method
8
Next generation sequencing methods - I
9
Next generation sequencing methods - II
10
Microbial whole genome sequencing: application in clinical microbiology and public health
11
Methods used in microorganism genotyping
12
Current methods in molecular microbiology laboratory
13
Technical problems and solution suggestions encountered in the methods used in molecular biology
14
Problems and solution suggestions regarding specificity, sensitivity and interpretation of results in molecular microbiology tests
Resources
1. Koneman's Color Atlas And Textbook of Diagnostic Microbiology. 7th edt. Jones & Bartlett Learning; 2016.
2. PCR Detection of Microbial Pathogens. Humana Press, 2016.
3. Molecular Microbiology: Diagnostic Principles and Practice. 3. Edt. ASM Press, 2016
Course Contribution to Program Qualifications
Course Contribution to Program Qualifications
No
Program Qualification
Contribution Level
1
2
3
4
5
1
Plans and statistically assesses scientific research, prepares an article for publication in a refereed journal and presents it in a scientific meeting.
X
2
Knows laboratory methods used in the field of Medical Microbiology and designs experimental studies on microorganisms.
X
3
Gains up-to-date scientific knowledge and skills in the field of microbiology and follows the innovations in this field.
X
4
Develops ability to use of devices and tools that serve routine analyses and research purposes in the medical microbiology laboratory
X
5
Statistically analyzes results of experiments at the expert level, (re)evaluates and convincingly defends, if necessary, and presents the results of scientific studies at the in writing or orally.
6
Develops ability to write grant proposals
X
7
Solves a scientific and technical problem.
X
8
Participates in meetings related to the field of Medical Microbiology, presents papers, participates in discussions, talks before audiance
X
9
Understands and interprets microbial diseases and their etiology at expert level.
X
Assessment Methods
Contribution Level
Absolute Evaluation
Rate of Midterm Exam to Success
50
Rate of Final Exam to Success
50
Total
100
ECTS / Workload Table
Activities
Number of
Duration(Hour)
Total Workload(Hour)
Course Hours
14
6
84
Guided Problem Solving
14
6
84
Resolution of Homework Problems and Submission as a Report
1
20
20
Term Project
0
0
0
Presentation of Project / Seminar
0
0
0
Quiz
0
0
0
Midterm Exam
7
8
56
General Exam
12
9
108
Performance Task, Maintenance Plan
12
7
84
Total Workload(Hour)
436
Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(436/30)
15
ECTS of the course: 30 hours of work is counted as 1 ECTS credit.
Detail Informations of the Course
Course Description
Course
Code
Semester
T+P (Hour)
Credit
ECTS
MOLECULAR TECHNIQUES in CLINICAL MICROBIOLOGY
-
Fall Semester
2+4
4
15
Course Program
Prerequisites Courses
Recommended Elective Courses
Language of Course
Turkish
Course Level
Third Cycle (Doctorate Degree)
Course Type
Elective
Course Coordinator
Assist.Prof. Özlem GÜVEN
Name of Lecturer(s)
Lect. Hatice Esra AĞEL
Assistant(s)
Aim
To explain the molecular biology techniques used in the detection and identification of microorganisms in samples and to determine antimicrobial resistance and to apply basic techniques.
Course Content
This course contains; Access to microorganism genetic information (websites, programs and usage application),Methods used in nucleic acid isolation,Polymerase chain reaction (PCR),Quantitative polymerase chain reaction (qPCR),Methods used in RNA detection; RT-PCR, RT-qPCR,Dijital PCR,Sanger sequencing method,Next generation sequencing methods - I,Next generation sequencing methods - II,Microbial whole genome sequencing: application in clinical microbiology and public health,Methods used in microorganism genotyping,Current methods in molecular microbiology laboratory,Technical problems and solution suggestions encountered in the methods used in molecular biology,Problems and solution suggestions regarding specificity, sensitivity and interpretation of results in molecular microbiology tests.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Explains molecular methods used in the field of microbiology and applies basic methods.
10, 16, 17, 9
A
Evaluates the problems encountered in molecular biology tests and offers solutions.
10, 16, 17, 9
A
Lists the sources of access to genetic information and the computer programs used.
Access to microorganism genetic information (websites, programs and usage application)
2
Methods used in nucleic acid isolation
3
Polymerase chain reaction (PCR)
4
Quantitative polymerase chain reaction (qPCR)
5
Methods used in RNA detection; RT-PCR, RT-qPCR
6
Dijital PCR
7
Sanger sequencing method
8
Next generation sequencing methods - I
9
Next generation sequencing methods - II
10
Microbial whole genome sequencing: application in clinical microbiology and public health
11
Methods used in microorganism genotyping
12
Current methods in molecular microbiology laboratory
13
Technical problems and solution suggestions encountered in the methods used in molecular biology
14
Problems and solution suggestions regarding specificity, sensitivity and interpretation of results in molecular microbiology tests
Resources
1. Koneman's Color Atlas And Textbook of Diagnostic Microbiology. 7th edt. Jones & Bartlett Learning; 2016.
2. PCR Detection of Microbial Pathogens. Humana Press, 2016.
3. Molecular Microbiology: Diagnostic Principles and Practice. 3. Edt. ASM Press, 2016
Course Contribution to Program Qualifications
Course Contribution to Program Qualifications
No
Program Qualification
Contribution Level
1
2
3
4
5
1
Plans and statistically assesses scientific research, prepares an article for publication in a refereed journal and presents it in a scientific meeting.
X
2
Knows laboratory methods used in the field of Medical Microbiology and designs experimental studies on microorganisms.
X
3
Gains up-to-date scientific knowledge and skills in the field of microbiology and follows the innovations in this field.
X
4
Develops ability to use of devices and tools that serve routine analyses and research purposes in the medical microbiology laboratory
X
5
Statistically analyzes results of experiments at the expert level, (re)evaluates and convincingly defends, if necessary, and presents the results of scientific studies at the in writing or orally.
6
Develops ability to write grant proposals
X
7
Solves a scientific and technical problem.
X
8
Participates in meetings related to the field of Medical Microbiology, presents papers, participates in discussions, talks before audiance
X
9
Understands and interprets microbial diseases and their etiology at expert level.
