Course Description
Course | Code | Semester | T+P (Hour) | Credit | ECTS |
---|
MOLECULAR TECHNIQUES in CLINICAL MICROBIOLOGY | MKBD2131390 | Fall Semester | 2+4 | 4 | 15 |
Prerequisites Courses | |
Recommended Elective Courses | |
Language of Course | Turkish |
Course Level | Third Cycle (Doctorate Degree) |
Course Type | Elective |
Course Coordinator | Assist.Prof. Özlem GÜVEN |
Name of Lecturer(s) | Assist.Prof. Özlem GÜVEN |
Assistant(s) | |
Aim | To explain the molecular biology techniques used in the detection and identification of microorganisms in samples and to determine antimicrobial resistance and to apply basic techniques. |
Course Content | This course contains; Access to microorganism genetic information (websites, programs and usage application),Methods used in nucleic acid isolation,Polymerase chain reaction (PCR),Quantitative polymerase chain reaction (qPCR),Methods used in RNA detection; RT-PCR, RT-qPCR,Dijital PCR,Sanger sequencing method,Next generation sequencing methods - I,Next generation sequencing methods - II,Microbial whole genome sequencing: application in clinical microbiology and public health,Methods used in microorganism genotyping,Current methods in molecular microbiology laboratory,Technical problems and solution suggestions encountered in the methods used in molecular biology,Problems and solution suggestions regarding specificity, sensitivity and interpretation of results in molecular microbiology tests. |
Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
Explains molecular methods used in the field of microbiology and applies basic methods. | 10, 16, 17, 9 | A |
Evaluates the problems encountered in molecular biology tests and offers solutions. | 10, 16, 17, 9 | A |
Lists the sources of access to genetic information and the computer programs used. | 16, 6, 9 | A |
Teaching Methods: | 10: Discussion Method, 16: Question - Answer Technique, 17: Experimental Technique, 6: Experiential Learning, 9: Lecture Method |
Assessment Methods: | A: Traditional Written Exam |
Course Outline
Order | Subjects | Preliminary Work |
---|
1 | Access to microorganism genetic information (websites, programs and usage application) | |
2 | Methods used in nucleic acid isolation | |
3 | Polymerase chain reaction (PCR) | |
4 | Quantitative polymerase chain reaction (qPCR) | |
5 | Methods used in RNA detection; RT-PCR, RT-qPCR | |
6 | Dijital PCR | |
7 | Sanger sequencing method | |
8 | Next generation sequencing methods - I | |
9 | Next generation sequencing methods - II | |
10 | Microbial whole genome sequencing: application in clinical microbiology and public health | |
11 | Methods used in microorganism genotyping | |
12 | Current methods in molecular microbiology laboratory | |
13 | Technical problems and solution suggestions encountered in the methods used in molecular biology | |
14 | Problems and solution suggestions regarding specificity, sensitivity and interpretation of results in molecular microbiology tests | |
Resources |
1. Koneman's Color Atlas And Textbook of Diagnostic Microbiology. 7th edt. Jones & Bartlett Learning; 2016.
2. PCR Detection of Microbial Pathogens. Humana Press, 2016.
3. Molecular Microbiology: Diagnostic Principles and Practice. 3. Edt. ASM Press, 2016 |
Course Contribution to Program Qualifications
Course Contribution to Program Qualifications |
No | Program Qualification | Contribution Level |
1 | 2 | 3 | 4 | 5 |
1 | Plans and statistically assesses scientific research, prepares an article for publication in a refereed journal and presents it in a scientific meeting. | | X | | | |
2 | Knows laboratory methods used in the field of Medical Microbiology and designs experimental studies on microorganisms. | | | | | X |
3 | Gains up-to-date scientific knowledge and skills in the field of microbiology and follows the innovations in this field. | | | X | | |
4 | Develops ability to use of devices and tools that serve routine analyses and research purposes in the medical microbiology laboratory | | | | | X |
5 | Statistically analyzes results of experiments at the expert level, (re)evaluates and convincingly defends, if necessary, and presents the results of scientific studies at the in writing or orally. | | | | | |
6 | Develops ability to write grant proposals | X | | | | |
7 | Solves a scientific and technical problem. | | | X | | |
8 | Participates in meetings related to the field of Medical Microbiology, presents papers, participates in discussions, talks before audiance | X | | | | |
9 | Understands and interprets microbial diseases and their etiology at expert level. | | X | | | |
Assessment Methods
Contribution Level | Absolute Evaluation |
Rate of Midterm Exam to Success | | 50 |
Rate of Final Exam to Success | | 50 |
Total | | 100 |
ECTS / Workload Table |
Activities | Number of | Duration(Hour) | Total Workload(Hour) |
Course Hours | 14 | 6 | 84 |
Guided Problem Solving | 14 | 6 | 84 |
Resolution of Homework Problems and Submission as a Report | 1 | 20 | 20 |
Term Project | 0 | 0 | 0 |
Presentation of Project / Seminar | 0 | 0 | 0 |
Quiz | 0 | 0 | 0 |
Midterm Exam | 7 | 8 | 56 |
General Exam | 12 | 9 | 108 |
Performance Task, Maintenance Plan | 12 | 7 | 84 |
Total Workload(Hour) | 436 |
Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(436/30) | 15 |
ECTS of the course: 30 hours of work is counted as 1 ECTS credit. |
Detail Informations of the Course
Course Description
Course | Code | Semester | T+P (Hour) | Credit | ECTS |
---|
MOLECULAR TECHNIQUES in CLINICAL MICROBIOLOGY | MKBD2131390 | Fall Semester | 2+4 | 4 | 15 |
Prerequisites Courses | |
Recommended Elective Courses | |
Language of Course | Turkish |
Course Level | Third Cycle (Doctorate Degree) |
Course Type | Elective |
Course Coordinator | Assist.Prof. Özlem GÜVEN |
Name of Lecturer(s) | Assist.Prof. Özlem GÜVEN |
Assistant(s) | |
Aim | To explain the molecular biology techniques used in the detection and identification of microorganisms in samples and to determine antimicrobial resistance and to apply basic techniques. |
Course Content | This course contains; Access to microorganism genetic information (websites, programs and usage application),Methods used in nucleic acid isolation,Polymerase chain reaction (PCR),Quantitative polymerase chain reaction (qPCR),Methods used in RNA detection; RT-PCR, RT-qPCR,Dijital PCR,Sanger sequencing method,Next generation sequencing methods - I,Next generation sequencing methods - II,Microbial whole genome sequencing: application in clinical microbiology and public health,Methods used in microorganism genotyping,Current methods in molecular microbiology laboratory,Technical problems and solution suggestions encountered in the methods used in molecular biology,Problems and solution suggestions regarding specificity, sensitivity and interpretation of results in molecular microbiology tests. |
Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
Explains molecular methods used in the field of microbiology and applies basic methods. | 10, 16, 17, 9 | A |
Evaluates the problems encountered in molecular biology tests and offers solutions. | 10, 16, 17, 9 | A |
Lists the sources of access to genetic information and the computer programs used. | 16, 6, 9 | A |
Teaching Methods: | 10: Discussion Method, 16: Question - Answer Technique, 17: Experimental Technique, 6: Experiential Learning, 9: Lecture Method |
Assessment Methods: | A: Traditional Written Exam |
Course Outline
Order | Subjects | Preliminary Work |
---|
1 | Access to microorganism genetic information (websites, programs and usage application) | |
2 | Methods used in nucleic acid isolation | |
3 | Polymerase chain reaction (PCR) | |
4 | Quantitative polymerase chain reaction (qPCR) | |
5 | Methods used in RNA detection; RT-PCR, RT-qPCR | |
6 | Dijital PCR | |
7 | Sanger sequencing method | |
8 | Next generation sequencing methods - I | |
9 | Next generation sequencing methods - II | |
10 | Microbial whole genome sequencing: application in clinical microbiology and public health | |
11 | Methods used in microorganism genotyping | |
12 | Current methods in molecular microbiology laboratory | |
13 | Technical problems and solution suggestions encountered in the methods used in molecular biology | |
14 | Problems and solution suggestions regarding specificity, sensitivity and interpretation of results in molecular microbiology tests | |
Resources |
1. Koneman's Color Atlas And Textbook of Diagnostic Microbiology. 7th edt. Jones & Bartlett Learning; 2016.
2. PCR Detection of Microbial Pathogens. Humana Press, 2016.
3. Molecular Microbiology: Diagnostic Principles and Practice. 3. Edt. ASM Press, 2016 |
Course Contribution to Program Qualifications
Course Contribution to Program Qualifications |
No | Program Qualification | Contribution Level |
1 | 2 | 3 | 4 | 5 |
1 | Plans and statistically assesses scientific research, prepares an article for publication in a refereed journal and presents it in a scientific meeting. | | X | | | |
2 | Knows laboratory methods used in the field of Medical Microbiology and designs experimental studies on microorganisms. | | | | | X |
3 | Gains up-to-date scientific knowledge and skills in the field of microbiology and follows the innovations in this field. | | | X | | |
4 | Develops ability to use of devices and tools that serve routine analyses and research purposes in the medical microbiology laboratory | | | | | X |
5 | Statistically analyzes results of experiments at the expert level, (re)evaluates and convincingly defends, if necessary, and presents the results of scientific studies at the in writing or orally. | | | | | |
6 | Develops ability to write grant proposals | X | | | | |
7 | Solves a scientific and technical problem. | | | X | | |
8 | Participates in meetings related to the field of Medical Microbiology, presents papers, participates in discussions, talks before audiance | X | | | | |
9 | Understands and interprets microbial diseases and their etiology at expert level. | | X | | | |
Assessment Methods
Contribution Level | Absolute Evaluation |
Rate of Midterm Exam to Success | | 50 |
Rate of Final Exam to Success | | 50 |
Total | | 100 |
Numerical Data
Ekleme Tarihi: 27/11/2023 - 03:39Son Güncelleme Tarihi: 27/11/2023 - 03:40
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