Ders Detayı
Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| ENSTRUMENTAL ANALYSIS in BIOCHEMISTRY II | - | Spring Semester | 2+2 | 3 | 6 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Third Cycle (Doctorate Degree) |
| Course Type | Elective |
| Course Coordinator | |
| Name of Lecturer(s) | Prof.Dr. Neslin EMEKLİ, Prof.Dr. Türkan YİĞİTBAŞI, Assoc.Prof. Sultan Sibel ERDEM, Assist.Prof. Gözde ÜLFER, Assist.Prof. Çağrı ÇAKICI, Assist.Prof. Derya CANSIZ |
| Assistant(s) | |
| Aim | To learn the appropriate test among analytical techniques, to be able to use them efficiently and be able to learn advanced principles of the techniques and tests |
| Course Content | This course contains; Introduction to one-spectrometric method,optical devices and components,atomic spectroscopy,fluorescence spectrometry,atomic emission spectrometry,atomic mass spectrometry,Atomic X ray spectrometry,Molecular Spectroscopy,UV-visible region,Molecular absorption,Molecular visible region,molecular luminescence spectrometry,Infraret spectrometry,Nuclear magnetic resonance spectrometry. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| At the end of this course, the students will be able to: 1-Question electromagnetic radiation and type of spectrophotometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.1Questions visible, UV, infrared, microwave and radio wave | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.2.Quesitons the reflection, scattering, polarisation and absorption of light | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.4.Question the spectrophotometry and photometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.5.Explains the spectrophotometry as a basic instrument | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.6.Explains the properties of preferred spectrometers the calibration of the spectrophotometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.7.Question nephelometry, fluorometry, atomic spectrometry, flame and infrared spectrometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2-Questions the mass spectrometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.1.Explains the principle and operational area of mass spectrometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.2.Explains the MALDİ-TOF | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.3.Question the using area of the MALDİ-TOF | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.4.Questions the SELDİ-TOF and using area | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.5.Explains the protein chip technology and relation to two-dimensional electrophoresis | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.6.Explains electrospray ionization mass spectrometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.7.Explains the relation of gas chromatography and mass spectrometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3-Question electroanalytical techniques and pH | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.1.Questions the electrochemical properties of solutions | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.2.Question the principle of electroanalytic techniques | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.3.Questions the operational knowledge and skills required for pH measurement | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4-Question nuclear magnetic resonance spectrometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.1.Explains magnetic fields and radio wavelength | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.3.Question the radioactive nucleus energy type used in nuclear magnetic resonance spectrometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.4.Explains applied area of nuclear magnetic resonance spectrometry | 11, 16, 19, 6, 9 | A, D, E |
| 1.3. Question the Lambert-Beer Law | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.8. Explains the relation of liquid chromatography and mass spectrometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.9. Questions properties of sequential mass spectrometry and using area | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.4. Question the importance of using area of pH measurement | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.2. Questions can electromagnetic field energy and level of nucleus energy | 10, 11, 16, 19, 6, 9 | E |
| 4.5. Questions of Elucidate enzyme protein structure by X-ray crystallography method | 10, 11, 16, 19, 6, 9 | A, D, E |
| Teaching Methods: | 10: Discussion Method, 11: Demonstration Method, 16: Question - Answer Technique, 19: Brainstorming Technique, 6: Experiential Learning, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, D: Oral Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | Introduction to one-spectrometric method | 1, 2 |
| 2 | optical devices and components | 1, 2 |
| 3 | atomic spectroscopy | 1, 2 |
| 4 | fluorescence spectrometry | 1, 2 |
| 5 | atomic emission spectrometry | 1, 2 |
| 6 | atomic mass spectrometry | 1, 2 |
| 7 | Atomic X ray spectrometry | 1, 2 |
| 8 | Molecular Spectroscopy | 1, 2 |
| 9 | UV-visible region | 1, 2 |
| 10 | Molecular absorption | 1, 2 |
| 11 | Molecular visible region | 1, 2 |
| 12 | molecular luminescence spectrometry | 1, 2 |
| 13 | Infraret spectrometry | 1, 2 |
| 14 | Nuclear magnetic resonance spectrometry | 1, 2 |
| Resources |
| 1) Clinical chemistry ; Theory , analysis , correlation ed.Lawrence A.Kaplan, Amadeo J.Pesce , Mosby Elsevier 5. Baskı, 2010Tietz, 2) Klinik kimyada temel ilkeler 5.baskı çeviri ed. Carl A. Burtis , ER, Ashwood çeviri ed. Diler Aslan Palme Yayıncılık, 2005 |
| Enstrümental Analiz ilkeleri (Prof.Dr.Sema Kılıç,Prof.Dr.Fitnat Köseoğlu,Doç.Dr.Hamza Yılmaz |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Defines macro and micromolecules in cell and body fluids at an advanced level. | X | |||||
| 2 | Knows how to store and protect the material to be analyzed. | X | |||||
| 3 | Knows how to identify molecules found in cells and body fluids. | X | |||||
| 4 | Knows the principles of technologies to be used for routine and research purposes. | X | |||||
| 5 | Knows how to evaluate the results of the study, interprets and questions. | X | |||||
| 6 | Knows how to use field-specific tools and equipment | X | |||||
| 7 | Knows how to access and interpret field-specific resources and information | X | |||||
| 8 | Shares information with disciplines related to the field | X | |||||
| 9 | Combines theoretical and factual knowledge with critical thoughts, conducts independent studies, evaluates the results, compares them with the literature, and gets them accepted by authors and scientific journals. | X | |||||
| 10 | Solves a scientific and technical problem, knows where to get help. | X | |||||
| 11 | Takes responsibility in joint scientific studies, contributes and works in harmony. | X | |||||
| 12 | Participates in meetings related to the field of biochemistry, presents papers, participates in discussions and congresses, publishes studies in reputable journals specific to the field. | X | |||||
| 13 | Collects, interprets, applies, announces data related to the field of biochemistry, conducts social and cultural studies, and supervises these studies scientifically and socially. | X | |||||
| 14 | Can take part in management activities, evaluate the results obtained to improve quality | ||||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |
| ECTS / Workload Table | ||||||
| Activities | Number of | Duration(Hour) | Total Workload(Hour) | |||
| Course Hours | 14 | 2 | 28 | |||
| Guided Problem Solving | 14 | 4 | 56 | |||
| Resolution of Homework Problems and Submission as a Report | 2 | 5 | 10 | |||
| Term Project | 2 | 5 | 10 | |||
| Presentation of Project / Seminar | 2 | 1 | 2 | |||
| Quiz | 0 | 0 | 0 | |||
| Midterm Exam | 1 | 25 | 25 | |||
| General Exam | 1 | 35 | 35 | |||
| Performance Task, Maintenance Plan | 0 | 0 | 0 | |||
| Total Workload(Hour) | 166 | |||||
| Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(166/30) | 6 | |||||
| ECTS of the course: 30 hours of work is counted as 1 ECTS credit. | ||||||
Detail Informations of the Course
Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| ENSTRUMENTAL ANALYSIS in BIOCHEMISTRY II | - | Spring Semester | 2+2 | 3 | 6 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Third Cycle (Doctorate Degree) |
| Course Type | Elective |
| Course Coordinator | |
| Name of Lecturer(s) | Prof.Dr. Neslin EMEKLİ, Prof.Dr. Türkan YİĞİTBAŞI, Assoc.Prof. Sultan Sibel ERDEM, Assist.Prof. Gözde ÜLFER, Assist.Prof. Çağrı ÇAKICI, Assist.Prof. Derya CANSIZ |
| Assistant(s) | |
| Aim | To learn the appropriate test among analytical techniques, to be able to use them efficiently and be able to learn advanced principles of the techniques and tests |
| Course Content | This course contains; Introduction to one-spectrometric method,optical devices and components,atomic spectroscopy,fluorescence spectrometry,atomic emission spectrometry,atomic mass spectrometry,Atomic X ray spectrometry,Molecular Spectroscopy,UV-visible region,Molecular absorption,Molecular visible region,molecular luminescence spectrometry,Infraret spectrometry,Nuclear magnetic resonance spectrometry. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| At the end of this course, the students will be able to: 1-Question electromagnetic radiation and type of spectrophotometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.1Questions visible, UV, infrared, microwave and radio wave | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.2.Quesitons the reflection, scattering, polarisation and absorption of light | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.4.Question the spectrophotometry and photometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.5.Explains the spectrophotometry as a basic instrument | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.6.Explains the properties of preferred spectrometers the calibration of the spectrophotometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.7.Question nephelometry, fluorometry, atomic spectrometry, flame and infrared spectrometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2-Questions the mass spectrometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.1.Explains the principle and operational area of mass spectrometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.2.Explains the MALDİ-TOF | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.3.Question the using area of the MALDİ-TOF | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.4.Questions the SELDİ-TOF and using area | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.5.Explains the protein chip technology and relation to two-dimensional electrophoresis | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.6.Explains electrospray ionization mass spectrometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.7.Explains the relation of gas chromatography and mass spectrometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3-Question electroanalytical techniques and pH | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.1.Questions the electrochemical properties of solutions | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.2.Question the principle of electroanalytic techniques | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.3.Questions the operational knowledge and skills required for pH measurement | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4-Question nuclear magnetic resonance spectrometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.1.Explains magnetic fields and radio wavelength | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.3.Question the radioactive nucleus energy type used in nuclear magnetic resonance spectrometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.4.Explains applied area of nuclear magnetic resonance spectrometry | 11, 16, 19, 6, 9 | A, D, E |
| 1.3. Question the Lambert-Beer Law | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.8. Explains the relation of liquid chromatography and mass spectrometry | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.9. Questions properties of sequential mass spectrometry and using area | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.4. Question the importance of using area of pH measurement | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.2. Questions can electromagnetic field energy and level of nucleus energy | 10, 11, 16, 19, 6, 9 | E |
| 4.5. Questions of Elucidate enzyme protein structure by X-ray crystallography method | 10, 11, 16, 19, 6, 9 | A, D, E |
| Teaching Methods: | 10: Discussion Method, 11: Demonstration Method, 16: Question - Answer Technique, 19: Brainstorming Technique, 6: Experiential Learning, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, D: Oral Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | Introduction to one-spectrometric method | 1, 2 |
| 2 | optical devices and components | 1, 2 |
| 3 | atomic spectroscopy | 1, 2 |
| 4 | fluorescence spectrometry | 1, 2 |
| 5 | atomic emission spectrometry | 1, 2 |
| 6 | atomic mass spectrometry | 1, 2 |
| 7 | Atomic X ray spectrometry | 1, 2 |
| 8 | Molecular Spectroscopy | 1, 2 |
| 9 | UV-visible region | 1, 2 |
| 10 | Molecular absorption | 1, 2 |
| 11 | Molecular visible region | 1, 2 |
| 12 | molecular luminescence spectrometry | 1, 2 |
| 13 | Infraret spectrometry | 1, 2 |
| 14 | Nuclear magnetic resonance spectrometry | 1, 2 |
| Resources |
| 1) Clinical chemistry ; Theory , analysis , correlation ed.Lawrence A.Kaplan, Amadeo J.Pesce , Mosby Elsevier 5. Baskı, 2010Tietz, 2) Klinik kimyada temel ilkeler 5.baskı çeviri ed. Carl A. Burtis , ER, Ashwood çeviri ed. Diler Aslan Palme Yayıncılık, 2005 |
| Enstrümental Analiz ilkeleri (Prof.Dr.Sema Kılıç,Prof.Dr.Fitnat Köseoğlu,Doç.Dr.Hamza Yılmaz |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Defines macro and micromolecules in cell and body fluids at an advanced level. | X | |||||
| 2 | Knows how to store and protect the material to be analyzed. | X | |||||
| 3 | Knows how to identify molecules found in cells and body fluids. | X | |||||
| 4 | Knows the principles of technologies to be used for routine and research purposes. | X | |||||
| 5 | Knows how to evaluate the results of the study, interprets and questions. | X | |||||
| 6 | Knows how to use field-specific tools and equipment | X | |||||
| 7 | Knows how to access and interpret field-specific resources and information | X | |||||
| 8 | Shares information with disciplines related to the field | X | |||||
| 9 | Combines theoretical and factual knowledge with critical thoughts, conducts independent studies, evaluates the results, compares them with the literature, and gets them accepted by authors and scientific journals. | X | |||||
| 10 | Solves a scientific and technical problem, knows where to get help. | X | |||||
| 11 | Takes responsibility in joint scientific studies, contributes and works in harmony. | X | |||||
| 12 | Participates in meetings related to the field of biochemistry, presents papers, participates in discussions and congresses, publishes studies in reputable journals specific to the field. | X | |||||
| 13 | Collects, interprets, applies, announces data related to the field of biochemistry, conducts social and cultural studies, and supervises these studies scientifically and socially. | X | |||||
| 14 | Can take part in management activities, evaluate the results obtained to improve quality | ||||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |