Course Supervisor: Prof. Dr Esra Çağavi, Course LaboAssistants: Laboratory supervisor from Research Institute for Health Sciences and Technologies (SABITA),
Aim
The aim of the Laboratory Rotation-II course is for Master’s level students to observe and be able to explain the basic technical approaches in laboratory application that will benefit their thesis work and understanding of the scope of different laboratory disciplines. By rotating in different labs every week, students are aimed to be exposed to the basic molecular biology techniques, proteomics and genomics applications, FACS, primary and cell line culture applications, transgenic animal models, electrophysiology, neuroscience and cancer research applications, basic protein and gene expression analysis.
Course Content
This course contains; Electroencephalography (EEG) and its use in scientific research,Electroencephalography (EEG) application and practice,Flourescence activated cell sorting (FACS) method,Functional Neurology Applications,Primary cells and cell line culture applications,Experimental and transgenic animal models and applications
,Evaluation of Laboratory Notebooks for the Midterm Exam,Drug screening and cell survival tests,Electrophysiology and patch-clamp method,Gene expression Analysis in Molecular Biology,Cancer Biology Lab and Applicaitons,Histology and cryosectioning technique,Protein Analysis in Molecular Biology,Evaluation of Laboratory Notebooks for the Final Exam.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Explains the culture methods for differen cell type, experimental transgenic animal models, basic neuroscience application as EEG and electrophsiology, basic approaches in cancer, cell survival analysis, FACS and tissue sectioning practices.
14, 17, 3, 9
A, E
Explain principles of different experimental techniques.
14, 17, 9
A, E
Define the principles of basic research laboratory equipments.
14, 17, 9
A, E
Explains how to process and interpret scientific data at the basic level.
14, 17, 9
A, E
Teaching Methods:
14: Self Study Method, 17: Experimental Technique, 3: Problem Baded Learning Model, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, E: Homework
Course Outline
Order
Subjects
Preliminary Work
1
Electroencephalography (EEG) and its use in scientific research
Self-study
2
Electroencephalography (EEG) application and practice
Self-study
3
Flourescence activated cell sorting (FACS) method
Self-study
4
Functional Neurology Applications
Self-study
5
Primary cells and cell line culture applications
Self-study
6
Experimental and transgenic animal models and applications
7
Evaluation of Laboratory Notebooks for the Midterm Exam
8
Drug screening and cell survival tests
9
Electrophysiology and patch-clamp method
10
Gene expression Analysis in Molecular Biology
11
Cancer Biology Lab and Applicaitons
12
Histology and cryosectioning technique
13
Protein Analysis in Molecular Biology
14
Evaluation of Laboratory Notebooks for the Final Exam
Course Supervisor: Prof. Dr Esra Çağavi, Course LaboAssistants: Laboratory supervisor from Research Institute for Health Sciences and Technologies (SABITA),
Aim
The aim of the Laboratory Rotation-II course is for Master’s level students to observe and be able to explain the basic technical approaches in laboratory application that will benefit their thesis work and understanding of the scope of different laboratory disciplines. By rotating in different labs every week, students are aimed to be exposed to the basic molecular biology techniques, proteomics and genomics applications, FACS, primary and cell line culture applications, transgenic animal models, electrophysiology, neuroscience and cancer research applications, basic protein and gene expression analysis.
Course Content
This course contains; Electroencephalography (EEG) and its use in scientific research,Electroencephalography (EEG) application and practice,Flourescence activated cell sorting (FACS) method,Functional Neurology Applications,Primary cells and cell line culture applications,Experimental and transgenic animal models and applications
,Evaluation of Laboratory Notebooks for the Midterm Exam,Drug screening and cell survival tests,Electrophysiology and patch-clamp method,Gene expression Analysis in Molecular Biology,Cancer Biology Lab and Applicaitons,Histology and cryosectioning technique,Protein Analysis in Molecular Biology,Evaluation of Laboratory Notebooks for the Final Exam.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Explains the culture methods for differen cell type, experimental transgenic animal models, basic neuroscience application as EEG and electrophsiology, basic approaches in cancer, cell survival analysis, FACS and tissue sectioning practices.
14, 17, 3, 9
A, E
Explain principles of different experimental techniques.
14, 17, 9
A, E
Define the principles of basic research laboratory equipments.
14, 17, 9
A, E
Explains how to process and interpret scientific data at the basic level.
14, 17, 9
A, E
Teaching Methods:
14: Self Study Method, 17: Experimental Technique, 3: Problem Baded Learning Model, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, E: Homework
Course Outline
Order
Subjects
Preliminary Work
1
Electroencephalography (EEG) and its use in scientific research
Self-study
2
Electroencephalography (EEG) application and practice
Self-study
3
Flourescence activated cell sorting (FACS) method
Self-study
4
Functional Neurology Applications
Self-study
5
Primary cells and cell line culture applications
Self-study
6
Experimental and transgenic animal models and applications
7
Evaluation of Laboratory Notebooks for the Midterm Exam
8
Drug screening and cell survival tests
9
Electrophysiology and patch-clamp method
10
Gene expression Analysis in Molecular Biology
11
Cancer Biology Lab and Applicaitons
12
Histology and cryosectioning technique
13
Protein Analysis in Molecular Biology
14
Evaluation of Laboratory Notebooks for the Final Exam
