Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| ENSTRUMENTAL ANALYSIS in BIOCHEMISTRY I | - | Spring Semester | 1+2 | 2 | 5 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Second Cycle (Master's Degree) |
| Course Type | Elective |
| Course Coordinator | Assoc.Prof. Sultan Sibel ERDEM |
| Name of Lecturer(s) | Prof.Dr. Neslin EMEKLİ, Prof.Dr. Türkan YİĞİTBAŞI, Assoc.Prof. Sultan Sibel ERDEM, Assist.Prof. Gözde ÜLFER, Assist.Prof. Çağrı ÇAKICI, Assist.Prof. Derya CANSIZ |
| Assistant(s) | |
| Aim | To be able to choose the appropriate test among analytical techniques, to be able to use them efficiently and be able to learn basic principles of the techniques and tests |
| Course Content | This course contains; Introduction to one-spectrometric method,Optical devices and components,Atomic spectroscopy,Fluorescence spectrometry,Atomic emission spectrometry,Atomic mass spectrometry,Atomic X ray spectrometry,Molecular Spectroscopy,UV-visible region,Molecular absorption,Molecular visible region,molecular luminescence spectrometry,Infraret spectrometry,Nuclear magnetic resonance spectrometry. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| 1-Explains electromagnetic radiation and various spectrophotometrics. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.1.Explains visible rays, X-rays, ultraviolet, infrared, microwave and radio waves. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.2.Explains reflection, scattering, polarization and absorption of light. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.4.Defines the concepts of spectrometry and photometry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.5.Defines the basic parts of the spectrophotometer. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.6.Defines the features of preferred spectrometers and calibrates the spectrophotometer. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.7.Explains nepholometry, fluorometry, atomic spectrometry, flame and infrared spectrometry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2-Can discuss mass spectrometry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.2.Explains the relationship between matrix-supported laser desorption/ionization time-of-flight mass spectrometry (MALDI) laser beams and the time of flight (TOF) of ions to the detector. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.3.Explains the usage areas of MALDİ-TOF. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.3.Question the using area of the MALDİ-TOF | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.5.Explains the relationship between protein chip technologies and the 2-dimensional electrophoresis method. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.6.Explains the features of electrospray ionization mass spectrometry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.7.Explains the relationship between gas chromatography and mass spectrometry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3-Defines electroanalytical methods and pH concepts. | 10, 11, 16, 19, 9 | A, D, E |
| 3.1.Explains the electrochemical properties of the solution. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.2.Explains the working principles of electroanalytical methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.4.Discuss the importance of usage areas of pH measurement. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4-Discuss the nuclear magnetic resonance spectrometer. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.1.Explains the relationship between radio wave and magnetic field. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.2.Explains the change in energy level in a charged nucleus placed in an electromagnetic field. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.3.Defines the types of radioactive nuclei such as hydrogen, carbon and nitrogen used in nuclear magnetic resonance spectrometry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.4.Describes the application areas of nuclear magnetic resonance spectrometry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.5.Explains enzyme-protein structures by X-ray crystallography method. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.3. Explain Lambert-Beer law. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.1. Explains the principle and working areas of mass spectrometry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.8. Explains the relationship between liquid chromatography and mass spectrometry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.9. Discusses the features and uses of tandem mass spectrometry (TANDEM). | 10, 11, 16, 6, 9 | A, D, E |
| 3.3. Has the necessary knowledge and skills for operational pH measurement. | 10, 11, 16, 19, 6, 9 | A, D, E |
| Teaching Methods: | 10: Discussion Method, 11: Demonstration Method, 16: Question - Answer Technique, 19: Brainstorming Technique, 6: Experiential Learning, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, D: Oral Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | Introduction to one-spectrometric method | 1, 2, 3, 4, 5 |
| 2 | Optical devices and components | 1, 2, 3, 4, 5 |
| 3 | Atomic spectroscopy | 1, 2, 3, 4, 5 |
| 4 | Fluorescence spectrometry | 1, 2, 3, 4, 5 |
| 5 | Atomic emission spectrometry | 1, 2, 3, 4, 5 |
| 6 | Atomic mass spectrometry | 1, 2, 3, 4, 5 |
| 7 | Atomic X ray spectrometry | 1, 2, 3, 4, 5 |
| 8 | Molecular Spectroscopy | 1, 2, 3, 4, 5 |
| 9 | UV-visible region | 1, 2, 3, 4, 5 |
| 10 | Molecular absorption | 1, 2, 3, 4, 5 |
| 11 | Molecular visible region | 1, 2, 3, 4, 5 |
| 12 | molecular luminescence spectrometry | 1, 2, 3, 4, 5 |
| 13 | Infraret spectrometry | 1, 2, 3, 4, 5 |
| 14 | Nuclear magnetic resonance spectrometry | 1, 2, 3, 4, 5 |
| Resources |
| Clinical chemistry ; Theory , analysis , correlation ed.Lawrence A.Kaplan, Amadeo J.Pesce , Mosby Elsevier 5. Baskı, 2010Tietz, Klinik kimyada temel ilkeler 5.baskı çeviri ed. Carl A. Burtis , ER, Ashwood çeviri ed. Diler Aslan Palme Yayıncılık, 2005 |
| Enstrümental Analiz ilkeleri(Prof.Dr.Sema Kılıç,Prof.Dr.Fitnat Köseoğlu,Doç.Dr.Hamza Yılmaz) |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Knows the biochemical macromolecular metabolism. Applies and advances the knowlegde. | ||||||
| 2 | Knows the principles of routine and research oriented biochemical technologies. | X | |||||
| 3 | Practical Laboratory Applications (Sampling conditions of various body fluids and tissue samples in health and disease states, reasons of analytical errors, storage conditions of samples,working principles of technologies used in routine and research based studies, determinig reference values.) | X | |||||
| 4 | Knows basic biostatistics methods, applies to experimental study results and evaluates. | ||||||
| 5 | Knows how to use specific instruments in biochemistry | X | |||||
| 6 | Can access printeed and electronic literature and able to follow the advancements in biochemistry and related field. | X | |||||
| 7 | Can integrate and interpret the knowledge gained in biochemistry with knowledge obtained in other fields and can generate new information. | X | |||||
| 8 | Independantly applies his/her theoretical knowledge, critical thinking skills to the area of study, evaluates the results and writes a report. | X | |||||
| 9 | Defines a technical or scientific problem either with the helpof an advisor or independently, can come up with proposals and solve when needed. | X | |||||
| 10 | Take on responsibility in collborative studies, contributes and work in harmony. | X | |||||
| 11 | Follows national and international literature in Biochemistry, presents the work in seminars or publishes in journals. | X | |||||
| 12 | Can evaluate and teach biochemical data collection, interpretation, application and decleration recpecting cultural, scientific, social and ethical values. | X | |||||
| 13 | Take responsibility in managing, evaluate the results according to the context of quality processes. | X | |||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |
| ECTS / Workload Table | ||||||
| Activities | Number of | Duration(Hour) | Total Workload(Hour) | |||
| Course Hours | 14 | 2 | 28 | |||
| Guided Problem Solving | 14 | 2 | 28 | |||
| Resolution of Homework Problems and Submission as a Report | 0 | 0 | 0 | |||
| Term Project | 2 | 5 | 10 | |||
| Presentation of Project / Seminar | 2 | 10 | 20 | |||
| Quiz | 0 | 0 | 0 | |||
| Midterm Exam | 1 | 20 | 20 | |||
| General Exam | 1 | 30 | 30 | |||
| Performance Task, Maintenance Plan | 0 | 0 | 0 | |||
| Total Workload(Hour) | 136 | |||||
| Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(136/30) | 5 | |||||
| ECTS of the course: 30 hours of work is counted as 1 ECTS credit. | ||||||
Detail Informations of the Course
Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| ENSTRUMENTAL ANALYSIS in BIOCHEMISTRY I | - | Spring Semester | 1+2 | 2 | 5 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Second Cycle (Master's Degree) |
| Course Type | Elective |
| Course Coordinator | Assoc.Prof. Sultan Sibel ERDEM |
| Name of Lecturer(s) | Prof.Dr. Neslin EMEKLİ, Prof.Dr. Türkan YİĞİTBAŞI, Assoc.Prof. Sultan Sibel ERDEM, Assist.Prof. Gözde ÜLFER, Assist.Prof. Çağrı ÇAKICI, Assist.Prof. Derya CANSIZ |
| Assistant(s) | |
| Aim | To be able to choose the appropriate test among analytical techniques, to be able to use them efficiently and be able to learn basic principles of the techniques and tests |
| Course Content | This course contains; Introduction to one-spectrometric method,Optical devices and components,Atomic spectroscopy,Fluorescence spectrometry,Atomic emission spectrometry,Atomic mass spectrometry,Atomic X ray spectrometry,Molecular Spectroscopy,UV-visible region,Molecular absorption,Molecular visible region,molecular luminescence spectrometry,Infraret spectrometry,Nuclear magnetic resonance spectrometry. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| 1-Explains electromagnetic radiation and various spectrophotometrics. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.1.Explains visible rays, X-rays, ultraviolet, infrared, microwave and radio waves. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.2.Explains reflection, scattering, polarization and absorption of light. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.4.Defines the concepts of spectrometry and photometry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.5.Defines the basic parts of the spectrophotometer. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.6.Defines the features of preferred spectrometers and calibrates the spectrophotometer. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.7.Explains nepholometry, fluorometry, atomic spectrometry, flame and infrared spectrometry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2-Can discuss mass spectrometry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.2.Explains the relationship between matrix-supported laser desorption/ionization time-of-flight mass spectrometry (MALDI) laser beams and the time of flight (TOF) of ions to the detector. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.3.Explains the usage areas of MALDİ-TOF. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.3.Question the using area of the MALDİ-TOF | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.5.Explains the relationship between protein chip technologies and the 2-dimensional electrophoresis method. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.6.Explains the features of electrospray ionization mass spectrometry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.7.Explains the relationship between gas chromatography and mass spectrometry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3-Defines electroanalytical methods and pH concepts. | 10, 11, 16, 19, 9 | A, D, E |
| 3.1.Explains the electrochemical properties of the solution. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.2.Explains the working principles of electroanalytical methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.4.Discuss the importance of usage areas of pH measurement. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4-Discuss the nuclear magnetic resonance spectrometer. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.1.Explains the relationship between radio wave and magnetic field. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.2.Explains the change in energy level in a charged nucleus placed in an electromagnetic field. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.3.Defines the types of radioactive nuclei such as hydrogen, carbon and nitrogen used in nuclear magnetic resonance spectrometry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.4.Describes the application areas of nuclear magnetic resonance spectrometry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.5.Explains enzyme-protein structures by X-ray crystallography method. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.3. Explain Lambert-Beer law. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.1. Explains the principle and working areas of mass spectrometry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.8. Explains the relationship between liquid chromatography and mass spectrometry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.9. Discusses the features and uses of tandem mass spectrometry (TANDEM). | 10, 11, 16, 6, 9 | A, D, E |
| 3.3. Has the necessary knowledge and skills for operational pH measurement. | 10, 11, 16, 19, 6, 9 | A, D, E |
| Teaching Methods: | 10: Discussion Method, 11: Demonstration Method, 16: Question - Answer Technique, 19: Brainstorming Technique, 6: Experiential Learning, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, D: Oral Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | Introduction to one-spectrometric method | 1, 2, 3, 4, 5 |
| 2 | Optical devices and components | 1, 2, 3, 4, 5 |
| 3 | Atomic spectroscopy | 1, 2, 3, 4, 5 |
| 4 | Fluorescence spectrometry | 1, 2, 3, 4, 5 |
| 5 | Atomic emission spectrometry | 1, 2, 3, 4, 5 |
| 6 | Atomic mass spectrometry | 1, 2, 3, 4, 5 |
| 7 | Atomic X ray spectrometry | 1, 2, 3, 4, 5 |
| 8 | Molecular Spectroscopy | 1, 2, 3, 4, 5 |
| 9 | UV-visible region | 1, 2, 3, 4, 5 |
| 10 | Molecular absorption | 1, 2, 3, 4, 5 |
| 11 | Molecular visible region | 1, 2, 3, 4, 5 |
| 12 | molecular luminescence spectrometry | 1, 2, 3, 4, 5 |
| 13 | Infraret spectrometry | 1, 2, 3, 4, 5 |
| 14 | Nuclear magnetic resonance spectrometry | 1, 2, 3, 4, 5 |
| Resources |
| Clinical chemistry ; Theory , analysis , correlation ed.Lawrence A.Kaplan, Amadeo J.Pesce , Mosby Elsevier 5. Baskı, 2010Tietz, Klinik kimyada temel ilkeler 5.baskı çeviri ed. Carl A. Burtis , ER, Ashwood çeviri ed. Diler Aslan Palme Yayıncılık, 2005 |
| Enstrümental Analiz ilkeleri(Prof.Dr.Sema Kılıç,Prof.Dr.Fitnat Köseoğlu,Doç.Dr.Hamza Yılmaz) |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Knows the biochemical macromolecular metabolism. Applies and advances the knowlegde. | ||||||
| 2 | Knows the principles of routine and research oriented biochemical technologies. | X | |||||
| 3 | Practical Laboratory Applications (Sampling conditions of various body fluids and tissue samples in health and disease states, reasons of analytical errors, storage conditions of samples,working principles of technologies used in routine and research based studies, determinig reference values.) | X | |||||
| 4 | Knows basic biostatistics methods, applies to experimental study results and evaluates. | ||||||
| 5 | Knows how to use specific instruments in biochemistry | X | |||||
| 6 | Can access printeed and electronic literature and able to follow the advancements in biochemistry and related field. | X | |||||
| 7 | Can integrate and interpret the knowledge gained in biochemistry with knowledge obtained in other fields and can generate new information. | X | |||||
| 8 | Independantly applies his/her theoretical knowledge, critical thinking skills to the area of study, evaluates the results and writes a report. | X | |||||
| 9 | Defines a technical or scientific problem either with the helpof an advisor or independently, can come up with proposals and solve when needed. | X | |||||
| 10 | Take on responsibility in collborative studies, contributes and work in harmony. | X | |||||
| 11 | Follows national and international literature in Biochemistry, presents the work in seminars or publishes in journals. | X | |||||
| 12 | Can evaluate and teach biochemical data collection, interpretation, application and decleration recpecting cultural, scientific, social and ethical values. | X | |||||
| 13 | Take responsibility in managing, evaluate the results according to the context of quality processes. | X | |||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |