Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| MOLECULAR BIOLOGICAL METHODS | - | Spring Semester | 2+0 | 2 | 4 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Short Cycle (Associate's Degree) |
| Course Type | Required |
| Course Coordinator | Assist.Prof. Elif Zeynep YILMAZ |
| Name of Lecturer(s) | Assist.Prof. Hilal EREN GÖZEL |
| Assistant(s) | None |
| Aim | Teaching the main principles of molecular biology techniques to the students |
| Course Content | This course contains; Molecular Biology Techniques: Overview,Introduction of lab materials and machines,Physical and chemical homogenization techniques,DNA isolation and analysis,RNA isolation and analysis,Polymerase chain reaction (PCR) and gene cloning,Gene sequencing,Protein isolation and purification,Determining the concentration of proteins and electrophoretic analysis,Methods based on hybridization of nucleic acids and proteins,Basic principles and methods for enzymatic assay to determine enzyme activity,Spectroscopic techniques,Cell culture,Microscopic applications. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| 1.Tell the idea of molecular biology techniques | 12, 13, 16, 9 | A |
| 2. Explain the field of application these techniques at the lab | 12, 13, 16, 9 | A |
| Teaching Methods: | 12: Problem Solving Method, 13: Case Study Method, 16: Question - Answer Technique, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | Molecular Biology Techniques: Overview | Studying the subject from the lecture notes given by the teaching staff. |
| 2 | Introduction of lab materials and machines | Studying the subject from the lecture notes given by the teaching staff. |
| 3 | Physical and chemical homogenization techniques | Studying the subject from the lecture notes given by the teaching staff. |
| 4 | DNA isolation and analysis | Studying the subject from the lecture notes given by the teaching staff. |
| 5 | RNA isolation and analysis | Studying the subject from the lecture notes given by the teaching staff. |
| 6 | Polymerase chain reaction (PCR) and gene cloning | Studying the subject from the lecture notes given by the teaching staff. |
| 7 | Gene sequencing | Studying the subject from the lecture notes given by the teaching staff. |
| 8 | Protein isolation and purification | Studying the subject from the lecture notes given by the teaching staff. |
| 9 | Determining the concentration of proteins and electrophoretic analysis | Studying the subject from the lecture notes given by the teaching staff. |
| 10 | Methods based on hybridization of nucleic acids and proteins | Studying the subject from the lecture notes given by the teaching staff. |
| 11 | Basic principles and methods for enzymatic assay to determine enzyme activity | Studying the subject from the lecture notes given by the teaching staff. |
| 12 | Spectroscopic techniques | Studying the subject from the lecture notes given by the teaching staff. |
| 13 | Cell culture | Studying the subject from the lecture notes given by the teaching staff. |
| 14 | Microscopic applications | Studying the subject from the lecture notes given by the teaching staff. |
| Resources |
| Instructor's lecture notes |
| Molecular Biology Techniques: An Intensive Laboratory Course (Walt Ream, Katharine G. Field ), 1999. Moleküler Biyolojide kullanılan yöntemler, Prof.Dr. Güler Temizkan, Prof.Dr.Nazlı Arda, Nobel Tıp Kitabevleri, 2008. |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | PC1. The student acquires theoretical and practical knowledge related to his field at a basic level. | X | |||||
| 2 | PC2.The student owns information about moral discipline and ethical rules related to his field. | X | |||||
| 3 | PC3. The student uses theoretical and practical knowledge related to his field at a basic level; basic fundamental computer programs and related technologies. | ||||||
| 4 | PC4. The student manages a duty independently by using the knowledge about his field at a basic level. | X | |||||
| 5 | PC5. The student possesses the consciousness of wholelife learning | ||||||
| 6 | PC6. The student evaluates the knowledge about his field at a basic level with a critical approach, he designates his learning needs and directs his learning. | ||||||
| 7 | PC7. The student is sensitive to and he monitors progress about the events related to his field of study and in the agenda of the society and the world. He informs relevant people and institutions by using his acquired basic knowledge and skills about these issues. | X | |||||
| 8 | PC8.The student supports the social environment projects and activities with a sense of social responsibility. | ||||||
| 9 | PC9.The student monitors the information about his field and communicates with his colleagues by using a foreign language at least at the European Language Portfolio A2 level. | ||||||
| 10 | PC10.The student uses information and communication technologies with at least at basic level of European Computer Using Licence basic level of computer software which his field of study requires. | ||||||
| 11 | PC11. The student contributes to the stages of data collection, interpretation, implementation and announcing the results related to his field of study in accordance with social, scientific, cultural and ethical values. | X | |||||
| 12 | PC12. The student complies with and contributes to quality management and processes. | ||||||
| 13 | PC13.The student has sufficient consciousness about individual and public health, environmental protection and work safety issues. | ||||||
| 14 | PC14.The student acts in accordance with laws, regulations, legislations and professional ethics related to individual duties, rights and responsibilities. | X | |||||
| 15 | PC15.The student exemplifies the community with his external appearance, demeanor, attitude and behavior. | ||||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 20 | |
| Rate of Final Exam to Success | 80 | |
| Total | 100 | |
| ECTS / Workload Table | ||||||
| Activities | Number of | Duration(Hour) | Total Workload(Hour) | |||
| Course Hours | 14 | 2 | 28 | |||
| Guided Problem Solving | 4 | 4 | 16 | |||
| Resolution of Homework Problems and Submission as a Report | 0 | 0 | 0 | |||
| Term Project | 0 | 0 | 0 | |||
| Presentation of Project / Seminar | 0 | 0 | 0 | |||
| Quiz | 0 | 0 | 0 | |||
| Midterm Exam | 1 | 30 | 30 | |||
| General Exam | 1 | 40 | 40 | |||
| Performance Task, Maintenance Plan | 0 | 0 | 0 | |||
| Total Workload(Hour) | 114 | |||||
| Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(114/30) | 4 | |||||
| ECTS of the course: 30 hours of work is counted as 1 ECTS credit. | ||||||
Detail Informations of the Course
Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| MOLECULAR BIOLOGICAL METHODS | - | Spring Semester | 2+0 | 2 | 4 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Short Cycle (Associate's Degree) |
| Course Type | Required |
| Course Coordinator | Assist.Prof. Elif Zeynep YILMAZ |
| Name of Lecturer(s) | Assist.Prof. Hilal EREN GÖZEL |
| Assistant(s) | None |
| Aim | Teaching the main principles of molecular biology techniques to the students |
| Course Content | This course contains; Molecular Biology Techniques: Overview,Introduction of lab materials and machines,Physical and chemical homogenization techniques,DNA isolation and analysis,RNA isolation and analysis,Polymerase chain reaction (PCR) and gene cloning,Gene sequencing,Protein isolation and purification,Determining the concentration of proteins and electrophoretic analysis,Methods based on hybridization of nucleic acids and proteins,Basic principles and methods for enzymatic assay to determine enzyme activity,Spectroscopic techniques,Cell culture,Microscopic applications. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| 1.Tell the idea of molecular biology techniques | 12, 13, 16, 9 | A |
| 2. Explain the field of application these techniques at the lab | 12, 13, 16, 9 | A |
| Teaching Methods: | 12: Problem Solving Method, 13: Case Study Method, 16: Question - Answer Technique, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | Molecular Biology Techniques: Overview | Studying the subject from the lecture notes given by the teaching staff. |
| 2 | Introduction of lab materials and machines | Studying the subject from the lecture notes given by the teaching staff. |
| 3 | Physical and chemical homogenization techniques | Studying the subject from the lecture notes given by the teaching staff. |
| 4 | DNA isolation and analysis | Studying the subject from the lecture notes given by the teaching staff. |
| 5 | RNA isolation and analysis | Studying the subject from the lecture notes given by the teaching staff. |
| 6 | Polymerase chain reaction (PCR) and gene cloning | Studying the subject from the lecture notes given by the teaching staff. |
| 7 | Gene sequencing | Studying the subject from the lecture notes given by the teaching staff. |
| 8 | Protein isolation and purification | Studying the subject from the lecture notes given by the teaching staff. |
| 9 | Determining the concentration of proteins and electrophoretic analysis | Studying the subject from the lecture notes given by the teaching staff. |
| 10 | Methods based on hybridization of nucleic acids and proteins | Studying the subject from the lecture notes given by the teaching staff. |
| 11 | Basic principles and methods for enzymatic assay to determine enzyme activity | Studying the subject from the lecture notes given by the teaching staff. |
| 12 | Spectroscopic techniques | Studying the subject from the lecture notes given by the teaching staff. |
| 13 | Cell culture | Studying the subject from the lecture notes given by the teaching staff. |
| 14 | Microscopic applications | Studying the subject from the lecture notes given by the teaching staff. |
| Resources |
| Instructor's lecture notes |
| Molecular Biology Techniques: An Intensive Laboratory Course (Walt Ream, Katharine G. Field ), 1999. Moleküler Biyolojide kullanılan yöntemler, Prof.Dr. Güler Temizkan, Prof.Dr.Nazlı Arda, Nobel Tıp Kitabevleri, 2008. |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | PC1. The student acquires theoretical and practical knowledge related to his field at a basic level. | X | |||||
| 2 | PC2.The student owns information about moral discipline and ethical rules related to his field. | X | |||||
| 3 | PC3. The student uses theoretical and practical knowledge related to his field at a basic level; basic fundamental computer programs and related technologies. | ||||||
| 4 | PC4. The student manages a duty independently by using the knowledge about his field at a basic level. | X | |||||
| 5 | PC5. The student possesses the consciousness of wholelife learning | ||||||
| 6 | PC6. The student evaluates the knowledge about his field at a basic level with a critical approach, he designates his learning needs and directs his learning. | ||||||
| 7 | PC7. The student is sensitive to and he monitors progress about the events related to his field of study and in the agenda of the society and the world. He informs relevant people and institutions by using his acquired basic knowledge and skills about these issues. | X | |||||
| 8 | PC8.The student supports the social environment projects and activities with a sense of social responsibility. | ||||||
| 9 | PC9.The student monitors the information about his field and communicates with his colleagues by using a foreign language at least at the European Language Portfolio A2 level. | ||||||
| 10 | PC10.The student uses information and communication technologies with at least at basic level of European Computer Using Licence basic level of computer software which his field of study requires. | ||||||
| 11 | PC11. The student contributes to the stages of data collection, interpretation, implementation and announcing the results related to his field of study in accordance with social, scientific, cultural and ethical values. | X | |||||
| 12 | PC12. The student complies with and contributes to quality management and processes. | ||||||
| 13 | PC13.The student has sufficient consciousness about individual and public health, environmental protection and work safety issues. | ||||||
| 14 | PC14.The student acts in accordance with laws, regulations, legislations and professional ethics related to individual duties, rights and responsibilities. | X | |||||
| 15 | PC15.The student exemplifies the community with his external appearance, demeanor, attitude and behavior. | ||||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 20 | |
| Rate of Final Exam to Success | 80 | |
| Total | 100 | |