Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| CELL CULTURE | - | Fall Semester | 2+4 | 4 | 8 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Third Cycle (Doctorate Degree) |
| Course Type | Elective |
| Course Coordinator | Prof.Dr. İlknur KESKİN |
| Name of Lecturer(s) | Prof.Dr. İlknur KESKİN, Assoc.Prof. Seda KARABULUT |
| Assistant(s) | İlknur Keskin, Seda Karabulut |
| Aim | The aim of this lecture is to acquire the basic and detailed knowledge of cell culture, cell culture techniques, cell lines and stem cell aspects. |
| Course Content | This course contains; Introduction to cell culture,Cell lines,Cell culture techniques,Primary culture and passaging,Cryopreservation of cell and cell lines,Applications of cell culture,Midterm exam,Introduction to stem cell ,Cell cycle of stem cells, stress and aging,Pluoripotent stem cells,embryonic source of multipotent stem cells,adult stem cells,cancer stem cells,Final exam. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| Defines basic concepts about cell culture | 14, 17, 9 | A |
| Explains basic information about cell lineages. | 14, 9 | A |
| Defines basic concepts related to cell culture techniques. | 14, 17, 9 | A |
| Explains the basic concepts of primary cell culture and passaging. | 14, 17, 9 | A |
| Explains the basic information about freezing and thawing of cells and cell lines. | 14, 17, 9 | A |
| Explains the usage areas of cell culture. | 14, 9 | A |
| Defines the basic concepts related to the concept of stem cells. | 14, 9 | A |
| Explains basic concepts about the life cycle of stem cells, stress and aging. | 14, 9 | A, E |
| Identifies pluripotent stem cells. | 14, 9 | A |
| Explains the basic information about embryo and fetus derived multipotent stem cells. | 14, 9 | A, E |
| Identifies adult stem cells. | 14, 9 | A |
| Explains the basic information about cancer stem cells. | 14, 9 | A, E |
| Teaching Methods: | 14: Self Study Method, 17: Experimental Technique, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | Introduction to cell culture | Reading the relevant course presentation |
| 2 | Cell lines | Reading the relevant course presentation |
| 3 | Cell culture techniques | Reading the relevant course presentation |
| 4 | Primary culture and passaging | Reading the relevant course presentation |
| 5 | Cryopreservation of cell and cell lines | Reading the relevant course presentation |
| 6 | Applications of cell culture | Reading the relevant course presentation |
| 7 | Midterm exam | - |
| 8 | Introduction to stem cell | Reading the relevant course presentation |
| 9 | Cell cycle of stem cells, stress and aging | Reading the relevant course presentation |
| 10 | Pluoripotent stem cells | Reading the relevant course presentation |
| 11 | embryonic source of multipotent stem cells | Reading the relevant course presentation |
| 12 | adult stem cells | Reading the relevant course presentation |
| 13 | cancer stem cells | - |
| 14 | Final exam |
| Resources |
| Lecture notes |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Develops and deepens current and advanced knowledge in the field of histology with original thought and research at the level of expertise and reaches original definitions that will bring innovation to the field. | X | |||||
| 2 | Defines cell and tissue at an advanced level (ultrastructural). | X | |||||
| 3 | Knows the embryological details about the development of systems at an advanced level. | ||||||
| 4 | Comprehends the interdisciplinary interaction that the field is related to; reach original results by using knowledge that requires expertise in analyzing, synthesizing and evaluating new and complex ideas. | ||||||
| 5 | Develops a new idea, method, design or application that brings innovation to the field, or applies a known idea, method, design or application to a different field, researches, comprehends, designs, adapts and applies an original subject. | X | |||||
| 6 | He/she writes the report of the research he/she participated in. It publishes in a national/internationally accepted peer-reviewed journal and presents it at scientific meetings. | X | |||||
| 7 | It contributes to the progress in its field by independently carrying out an original work that brings innovations to its field, develops a new idea, method, design or application, or applies a known idea, method, design or application to a different field. | X | |||||
| 8 | Develops new thoughts and methods related to the field by using high-level mental processes such as creative and critical thinking, problem solving and decision making. | ||||||
| 9 | Participates in meetings related to the field of histology, presents papers, participates in discussions, gives conferences, and publishes his studies in reputable journals specific to the field. | ||||||
| 10 | Collects, interprets, applies and announces data related to histology and embryology. | X | |||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |
| ECTS / Workload Table | ||||||
| Activities | Number of | Duration(Hour) | Total Workload(Hour) | |||
| Course Hours | 14 | 5 | 70 | |||
| Guided Problem Solving | 14 | 8 | 112 | |||
| Resolution of Homework Problems and Submission as a Report | 3 | 10 | 30 | |||
| Term Project | 0 | 0 | 0 | |||
| Presentation of Project / Seminar | 0 | 0 | 0 | |||
| Quiz | 0 | 0 | 0 | |||
| Midterm Exam | 1 | 10 | 10 | |||
| General Exam | 1 | 10 | 10 | |||
| Performance Task, Maintenance Plan | 0 | 0 | 0 | |||
| Total Workload(Hour) | 232 | |||||
| Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(232/30) | 8 | |||||
| ECTS of the course: 30 hours of work is counted as 1 ECTS credit. | ||||||
Detail Informations of the Course
Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| CELL CULTURE | - | Fall Semester | 2+4 | 4 | 8 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Third Cycle (Doctorate Degree) |
| Course Type | Elective |
| Course Coordinator | Prof.Dr. İlknur KESKİN |
| Name of Lecturer(s) | Prof.Dr. İlknur KESKİN, Assoc.Prof. Seda KARABULUT |
| Assistant(s) | İlknur Keskin, Seda Karabulut |
| Aim | The aim of this lecture is to acquire the basic and detailed knowledge of cell culture, cell culture techniques, cell lines and stem cell aspects. |
| Course Content | This course contains; Introduction to cell culture,Cell lines,Cell culture techniques,Primary culture and passaging,Cryopreservation of cell and cell lines,Applications of cell culture,Midterm exam,Introduction to stem cell ,Cell cycle of stem cells, stress and aging,Pluoripotent stem cells,embryonic source of multipotent stem cells,adult stem cells,cancer stem cells,Final exam. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| Defines basic concepts about cell culture | 14, 17, 9 | A |
| Explains basic information about cell lineages. | 14, 9 | A |
| Defines basic concepts related to cell culture techniques. | 14, 17, 9 | A |
| Explains the basic concepts of primary cell culture and passaging. | 14, 17, 9 | A |
| Explains the basic information about freezing and thawing of cells and cell lines. | 14, 17, 9 | A |
| Explains the usage areas of cell culture. | 14, 9 | A |
| Defines the basic concepts related to the concept of stem cells. | 14, 9 | A |
| Explains basic concepts about the life cycle of stem cells, stress and aging. | 14, 9 | A, E |
| Identifies pluripotent stem cells. | 14, 9 | A |
| Explains the basic information about embryo and fetus derived multipotent stem cells. | 14, 9 | A, E |
| Identifies adult stem cells. | 14, 9 | A |
| Explains the basic information about cancer stem cells. | 14, 9 | A, E |
| Teaching Methods: | 14: Self Study Method, 17: Experimental Technique, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | Introduction to cell culture | Reading the relevant course presentation |
| 2 | Cell lines | Reading the relevant course presentation |
| 3 | Cell culture techniques | Reading the relevant course presentation |
| 4 | Primary culture and passaging | Reading the relevant course presentation |
| 5 | Cryopreservation of cell and cell lines | Reading the relevant course presentation |
| 6 | Applications of cell culture | Reading the relevant course presentation |
| 7 | Midterm exam | - |
| 8 | Introduction to stem cell | Reading the relevant course presentation |
| 9 | Cell cycle of stem cells, stress and aging | Reading the relevant course presentation |
| 10 | Pluoripotent stem cells | Reading the relevant course presentation |
| 11 | embryonic source of multipotent stem cells | Reading the relevant course presentation |
| 12 | adult stem cells | Reading the relevant course presentation |
| 13 | cancer stem cells | - |
| 14 | Final exam |
| Resources |
| Lecture notes |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Develops and deepens current and advanced knowledge in the field of histology with original thought and research at the level of expertise and reaches original definitions that will bring innovation to the field. | X | |||||
| 2 | Defines cell and tissue at an advanced level (ultrastructural). | X | |||||
| 3 | Knows the embryological details about the development of systems at an advanced level. | ||||||
| 4 | Comprehends the interdisciplinary interaction that the field is related to; reach original results by using knowledge that requires expertise in analyzing, synthesizing and evaluating new and complex ideas. | ||||||
| 5 | Develops a new idea, method, design or application that brings innovation to the field, or applies a known idea, method, design or application to a different field, researches, comprehends, designs, adapts and applies an original subject. | X | |||||
| 6 | He/she writes the report of the research he/she participated in. It publishes in a national/internationally accepted peer-reviewed journal and presents it at scientific meetings. | X | |||||
| 7 | It contributes to the progress in its field by independently carrying out an original work that brings innovations to its field, develops a new idea, method, design or application, or applies a known idea, method, design or application to a different field. | X | |||||
| 8 | Develops new thoughts and methods related to the field by using high-level mental processes such as creative and critical thinking, problem solving and decision making. | ||||||
| 9 | Participates in meetings related to the field of histology, presents papers, participates in discussions, gives conferences, and publishes his studies in reputable journals specific to the field. | ||||||
| 10 | Collects, interprets, applies and announces data related to histology and embryology. | X | |||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |