Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| ADVANCED HISTOCHEMICAL TECHNIQUES II | - | Spring Semester | 2+4 | 4 | 8 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Third Cycle (Doctorate Degree) |
| Course Type | Elective |
| Course Coordinator | Assist.Prof. Bircan KOLBAŞI |
| Name of Lecturer(s) | Prof.Dr. İlknur KESKİN, Assist.Prof. Bircan KOLBAŞI |
| Assistant(s) | İlknur Keskin, Seda Karabulut |
| Aim | Teach the advanced molecular diagnostic methods such as immunohistochemistry, immunocytochemistry, immunofluorescence, electron microscopy protocols used in histology laboratories. |
| Course Content | This course contains; Transmission electron microscope tissue tracing stages,Transmission and scanning electron microscope tissue tracking fixation and fixative types,Transmission electron microscope tissue embedding materials,Practice,Thick sectioning and staining methods,Thin sectioning and staining methods,Midterm exam,Problems encountered and solution suggestions,Scanning electron microscope tissue tracking stages,Stages of immunogold methods,Immungold image analysis,Problems encountered and solution suggestions-1,Problems encountered and solution suggestions-2,Final exam. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| Analyzes the prepared preparations. | 14, 9 | A |
| Explains the steps of scanning electron microscopy tissue processing. | 14, 9 | A |
| Explains the steps of the immunogold method. | 14, 9 | A, E |
| Identifies the problems that may be encountered during the immunogold method. | 14, 9 | A |
| It solves the problems that may be encountered during the Immungold method. | 14, 9 | A |
| It determines the problems that may be encountered during the electron microscopy tissue processing steps. | 14, 9 | A |
| Electron microscopy solves the problems that may be encountered during tissue processing steps. | 14, 9 | A |
| Explain the difference between scanning and transmission electron microscopy. | 14, 9 | A, E |
| Explains the steps of transmission electron microscopy tissue processing. | 14, 9 | A, E |
| Teaching Methods: | 14: Self Study Method, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | Transmission electron microscope tissue tracing stages | Conducting a literature review. |
| 2 | Transmission and scanning electron microscope tissue tracking fixation and fixative types | Conducting a literature review. |
| 3 | Transmission electron microscope tissue embedding materials | Conducting a literature review. |
| 4 | Practice | |
| 5 | Thick sectioning and staining methods | Conducting a literature review. |
| 6 | Thin sectioning and staining methods | Conducting a literature review. |
| 7 | Midterm exam | - |
| 8 | Problems encountered and solution suggestions | Conducting a literature review. |
| 9 | Scanning electron microscope tissue tracking stages | Conducting a literature review. |
| 10 | Stages of immunogold methods | Conducting a literature review. |
| 11 | Immungold image analysis | - |
| 12 | Problems encountered and solution suggestions-1 | Conducting a literature review. |
| 13 | Problems encountered and solution suggestions-2 | Conducting a literature review. |
| 14 | Final exam | - |
| Resources |
| Scientific research |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Develops and deepens current and advanced knowledge in the field of histology with original thought and research at the level of expertise and reaches original definitions that will bring innovation to the field. | X | |||||
| 2 | Defines cell and tissue at an advanced level (ultrastructural). | X | |||||
| 3 | Knows the embryological details about the development of systems at an advanced level. | ||||||
| 4 | Comprehends the interdisciplinary interaction that the field is related to; reach original results by using knowledge that requires expertise in analyzing, synthesizing and evaluating new and complex ideas. | X | |||||
| 5 | Develops a new idea, method, design or application that brings innovation to the field, or applies a known idea, method, design or application to a different field, researches, comprehends, designs, adapts and applies an original subject. | X | |||||
| 6 | He/she writes the report of the research he/she participated in. It publishes in a national/internationally accepted peer-reviewed journal and presents it at scientific meetings. | X | |||||
| 7 | It contributes to the progress in its field by independently carrying out an original work that brings innovations to its field, develops a new idea, method, design or application, or applies a known idea, method, design or application to a different field. | X | |||||
| 8 | Develops new thoughts and methods related to the field by using high-level mental processes such as creative and critical thinking, problem solving and decision making. | X | |||||
| 9 | Participates in meetings related to the field of histology, presents papers, participates in discussions, gives conferences, and publishes his studies in reputable journals specific to the field. | X | |||||
| 10 | Collects, interprets, applies and announces data related to histology and embryology. | ||||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |
| ECTS / Workload Table | ||||||
| Activities | Number of | Duration(Hour) | Total Workload(Hour) | |||
| Course Hours | 14 | 4 | 56 | |||
| Guided Problem Solving | 14 | 7 | 98 | |||
| Resolution of Homework Problems and Submission as a Report | 3 | 10 | 30 | |||
| Term Project | 0 | 0 | 0 | |||
| Presentation of Project / Seminar | 0 | 0 | 0 | |||
| Quiz | 0 | 0 | 0 | |||
| Midterm Exam | 1 | 10 | 10 | |||
| General Exam | 1 | 10 | 10 | |||
| Performance Task, Maintenance Plan | 14 | 2 | 28 | |||
| Total Workload(Hour) | 232 | |||||
| Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(232/30) | 8 | |||||
| ECTS of the course: 30 hours of work is counted as 1 ECTS credit. | ||||||
Detail Informations of the Course
Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| ADVANCED HISTOCHEMICAL TECHNIQUES II | - | Spring Semester | 2+4 | 4 | 8 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Third Cycle (Doctorate Degree) |
| Course Type | Elective |
| Course Coordinator | Assist.Prof. Bircan KOLBAŞI |
| Name of Lecturer(s) | Prof.Dr. İlknur KESKİN, Assist.Prof. Bircan KOLBAŞI |
| Assistant(s) | İlknur Keskin, Seda Karabulut |
| Aim | Teach the advanced molecular diagnostic methods such as immunohistochemistry, immunocytochemistry, immunofluorescence, electron microscopy protocols used in histology laboratories. |
| Course Content | This course contains; Transmission electron microscope tissue tracing stages,Transmission and scanning electron microscope tissue tracking fixation and fixative types,Transmission electron microscope tissue embedding materials,Practice,Thick sectioning and staining methods,Thin sectioning and staining methods,Midterm exam,Problems encountered and solution suggestions,Scanning electron microscope tissue tracking stages,Stages of immunogold methods,Immungold image analysis,Problems encountered and solution suggestions-1,Problems encountered and solution suggestions-2,Final exam. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| Analyzes the prepared preparations. | 14, 9 | A |
| Explains the steps of scanning electron microscopy tissue processing. | 14, 9 | A |
| Explains the steps of the immunogold method. | 14, 9 | A, E |
| Identifies the problems that may be encountered during the immunogold method. | 14, 9 | A |
| It solves the problems that may be encountered during the Immungold method. | 14, 9 | A |
| It determines the problems that may be encountered during the electron microscopy tissue processing steps. | 14, 9 | A |
| Electron microscopy solves the problems that may be encountered during tissue processing steps. | 14, 9 | A |
| Explain the difference between scanning and transmission electron microscopy. | 14, 9 | A, E |
| Explains the steps of transmission electron microscopy tissue processing. | 14, 9 | A, E |
| Teaching Methods: | 14: Self Study Method, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | Transmission electron microscope tissue tracing stages | Conducting a literature review. |
| 2 | Transmission and scanning electron microscope tissue tracking fixation and fixative types | Conducting a literature review. |
| 3 | Transmission electron microscope tissue embedding materials | Conducting a literature review. |
| 4 | Practice | |
| 5 | Thick sectioning and staining methods | Conducting a literature review. |
| 6 | Thin sectioning and staining methods | Conducting a literature review. |
| 7 | Midterm exam | - |
| 8 | Problems encountered and solution suggestions | Conducting a literature review. |
| 9 | Scanning electron microscope tissue tracking stages | Conducting a literature review. |
| 10 | Stages of immunogold methods | Conducting a literature review. |
| 11 | Immungold image analysis | - |
| 12 | Problems encountered and solution suggestions-1 | Conducting a literature review. |
| 13 | Problems encountered and solution suggestions-2 | Conducting a literature review. |
| 14 | Final exam | - |
| Resources |
| Scientific research |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Develops and deepens current and advanced knowledge in the field of histology with original thought and research at the level of expertise and reaches original definitions that will bring innovation to the field. | X | |||||
| 2 | Defines cell and tissue at an advanced level (ultrastructural). | X | |||||
| 3 | Knows the embryological details about the development of systems at an advanced level. | ||||||
| 4 | Comprehends the interdisciplinary interaction that the field is related to; reach original results by using knowledge that requires expertise in analyzing, synthesizing and evaluating new and complex ideas. | X | |||||
| 5 | Develops a new idea, method, design or application that brings innovation to the field, or applies a known idea, method, design or application to a different field, researches, comprehends, designs, adapts and applies an original subject. | X | |||||
| 6 | He/she writes the report of the research he/she participated in. It publishes in a national/internationally accepted peer-reviewed journal and presents it at scientific meetings. | X | |||||
| 7 | It contributes to the progress in its field by independently carrying out an original work that brings innovations to its field, develops a new idea, method, design or application, or applies a known idea, method, design or application to a different field. | X | |||||
| 8 | Develops new thoughts and methods related to the field by using high-level mental processes such as creative and critical thinking, problem solving and decision making. | X | |||||
| 9 | Participates in meetings related to the field of histology, presents papers, participates in discussions, gives conferences, and publishes his studies in reputable journals specific to the field. | X | |||||
| 10 | Collects, interprets, applies and announces data related to histology and embryology. | ||||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |