Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| LABORATORY ROTATION II | - | Spring Semester | 0+0 | - | 6 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Third Cycle (Doctorate Degree) |
| Course Type | Elective |
| Course Coordinator | Prof.Dr. Esra ÇAĞAVİ |
| Name of Lecturer(s) | Prof.Dr. Lütfü HANOĞLU, Prof.Dr. Gürkan ÖZTÜRK, Prof.Dr. Esra ÇAĞAVİ |
| Assistant(s) | Course Supervisor: Prof. Dr Esra Çağavi, Course LaboAssistants: Laboratory supervisor from Research Institute for Health Sciences and Technologies (SABITA), |
| Aim | The aim of the “Laboratory Rotation-II” course is for Ph.D. level students to to explain the advanced technical approaches in laboratory application that is expected to incorporate in their thesis work understanding of the scope of different laboratory disciplines. By rotating in different labs every week, students are aimed to be exposed to the advanced molecular biology techniques, proteomics and genomics applications, FACS, primary and cell line culture applications, transgenic animal models, electrophysiology, neuroscience and cancer research applications, basic protein and gene expression analysis. |
| Course Content | This course contains; Electroencephalography (EEG) recording principles ,EEG application and signal analysis,Flourescence activated cell sorting (FACS) method and analysis ,Functional Neurology Applications,Flourescence activated cell sorting (FACS) method and analysis ,Experimental and transgenic animal models and applications, Evaluation of Laboratory Notebooks for the Midterm Exam,Cell survival tests analysis,Electrophysiology applications and analysis,Quantitative RTPCR analysis in Molecular Biology,Cancer Biology Lab and Applications,Immunohistochemistry and cryosectioning ,SDS-PAGE and Western blot analysis,Evaluation of Laboratory Notebooks for the Final Exam. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| Explain the advanced culture methods, advanced approaches in molecular biology, experimental transgenic animal models and their applications, applications in neuroscience research, EEG principles and electrophsiology, fundamental approaches in cancer biology, cell survival analysis and FACS analysis. | 14, 17, 3, 9 | A, E |
| Explains advanced research techniques. | 14, 17, 3, 9 | A, E |
| Explain and provide solutions to various different experimental techniques. | 14, 17, 3, 9 | A, E |
| Define the principles of basic research laboratory equipments and can operate these devices. | 14, 17, 3, 9 | A, E |
| Explains the advanced data analysis approaches of scientific data. | 14, 17, 3, 9 | A, E |
| Teaching Methods: | 14: Self Study Method, 17: Experimental Technique, 3: Problem Baded Learning Model, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | Electroencephalography (EEG) recording principles | Self-study |
| 2 | EEG application and signal analysis | |
| 3 | Flourescence activated cell sorting (FACS) method and analysis | Self-study |
| 4 | Functional Neurology Applications | Self-study |
| 5 | Flourescence activated cell sorting (FACS) method and analysis | Self-study |
| 6 | Experimental and transgenic animal models and applications | Self-study |
| 7 | Evaluation of Laboratory Notebooks for the Midterm Exam | Self-study |
| 8 | Cell survival tests analysis | |
| 9 | Electrophysiology applications and analysis | |
| 10 | Quantitative RTPCR analysis in Molecular Biology | |
| 11 | Cancer Biology Lab and Applications | |
| 12 | Immunohistochemistry and cryosectioning | |
| 13 | SDS-PAGE and Western blot analysis | |
| 14 | Evaluation of Laboratory Notebooks for the Final Exam | Self-study |
| Resources |
| Laboratory Biosafety Manual (third edition) 1. Containment of biohazard - methods ISBN 92 4 154650 6 (LC/NLM classification: QY 25) WHO/ CDS/ CSR/ LYO/ 2004. 11 |
| Internet database |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Develops and deepens current and advanced knowledge in the field of histology with original thought and research at the level of expertise and reaches original definitions that will bring innovation to the field. | ||||||
| 2 | Defines cell and tissue at an advanced level (ultrastructural). | ||||||
| 3 | Knows the embryological details about the development of systems at an advanced level. | ||||||
| 4 | Comprehends the interdisciplinary interaction that the field is related to; reach original results by using knowledge that requires expertise in analyzing, synthesizing and evaluating new and complex ideas. | ||||||
| 5 | Develops a new idea, method, design or application that brings innovation to the field, or applies a known idea, method, design or application to a different field, researches, comprehends, designs, adapts and applies an original subject. | ||||||
| 6 | He/she writes the report of the research he/she participated in. It publishes in a national/internationally accepted peer-reviewed journal and presents it at scientific meetings. | ||||||
| 7 | It contributes to the progress in its field by independently carrying out an original work that brings innovations to its field, develops a new idea, method, design or application, or applies a known idea, method, design or application to a different field. | ||||||
| 8 | Develops new thoughts and methods related to the field by using high-level mental processes such as creative and critical thinking, problem solving and decision making. | ||||||
| 9 | Participates in meetings related to the field of histology, presents papers, participates in discussions, gives conferences, and publishes his studies in reputable journals specific to the field. | ||||||
| 10 | Collects, interprets, applies and announces data related to histology and embryology. | ||||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |
| ECTS / Workload Table | ||||||
| Activities | Number of | Duration(Hour) | Total Workload(Hour) | |||
| Course Hours | 14 | 3 | 42 | |||
| Guided Problem Solving | 5 | 6 | 30 | |||
| Resolution of Homework Problems and Submission as a Report | 14 | 6 | 84 | |||
| Term Project | 0 | 0 | 0 | |||
| Presentation of Project / Seminar | 0 | 0 | 0 | |||
| Quiz | 0 | 0 | 0 | |||
| Midterm Exam | 1 | 10 | 10 | |||
| General Exam | 1 | 10 | 10 | |||
| Performance Task, Maintenance Plan | 0 | 0 | 0 | |||
| Total Workload(Hour) | 176 | |||||
| Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(176/30) | 6 | |||||
| ECTS of the course: 30 hours of work is counted as 1 ECTS credit. | ||||||
Detail Informations of the Course
Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| LABORATORY ROTATION II | - | Spring Semester | 0+0 | - | 6 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Third Cycle (Doctorate Degree) |
| Course Type | Elective |
| Course Coordinator | Prof.Dr. Esra ÇAĞAVİ |
| Name of Lecturer(s) | Prof.Dr. Lütfü HANOĞLU, Prof.Dr. Gürkan ÖZTÜRK, Prof.Dr. Esra ÇAĞAVİ |
| Assistant(s) | Course Supervisor: Prof. Dr Esra Çağavi, Course LaboAssistants: Laboratory supervisor from Research Institute for Health Sciences and Technologies (SABITA), |
| Aim | The aim of the “Laboratory Rotation-II” course is for Ph.D. level students to to explain the advanced technical approaches in laboratory application that is expected to incorporate in their thesis work understanding of the scope of different laboratory disciplines. By rotating in different labs every week, students are aimed to be exposed to the advanced molecular biology techniques, proteomics and genomics applications, FACS, primary and cell line culture applications, transgenic animal models, electrophysiology, neuroscience and cancer research applications, basic protein and gene expression analysis. |
| Course Content | This course contains; Electroencephalography (EEG) recording principles ,EEG application and signal analysis,Flourescence activated cell sorting (FACS) method and analysis ,Functional Neurology Applications,Flourescence activated cell sorting (FACS) method and analysis ,Experimental and transgenic animal models and applications, Evaluation of Laboratory Notebooks for the Midterm Exam,Cell survival tests analysis,Electrophysiology applications and analysis,Quantitative RTPCR analysis in Molecular Biology,Cancer Biology Lab and Applications,Immunohistochemistry and cryosectioning ,SDS-PAGE and Western blot analysis,Evaluation of Laboratory Notebooks for the Final Exam. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| Explain the advanced culture methods, advanced approaches in molecular biology, experimental transgenic animal models and their applications, applications in neuroscience research, EEG principles and electrophsiology, fundamental approaches in cancer biology, cell survival analysis and FACS analysis. | 14, 17, 3, 9 | A, E |
| Explains advanced research techniques. | 14, 17, 3, 9 | A, E |
| Explain and provide solutions to various different experimental techniques. | 14, 17, 3, 9 | A, E |
| Define the principles of basic research laboratory equipments and can operate these devices. | 14, 17, 3, 9 | A, E |
| Explains the advanced data analysis approaches of scientific data. | 14, 17, 3, 9 | A, E |
| Teaching Methods: | 14: Self Study Method, 17: Experimental Technique, 3: Problem Baded Learning Model, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | Electroencephalography (EEG) recording principles | Self-study |
| 2 | EEG application and signal analysis | |
| 3 | Flourescence activated cell sorting (FACS) method and analysis | Self-study |
| 4 | Functional Neurology Applications | Self-study |
| 5 | Flourescence activated cell sorting (FACS) method and analysis | Self-study |
| 6 | Experimental and transgenic animal models and applications | Self-study |
| 7 | Evaluation of Laboratory Notebooks for the Midterm Exam | Self-study |
| 8 | Cell survival tests analysis | |
| 9 | Electrophysiology applications and analysis | |
| 10 | Quantitative RTPCR analysis in Molecular Biology | |
| 11 | Cancer Biology Lab and Applications | |
| 12 | Immunohistochemistry and cryosectioning | |
| 13 | SDS-PAGE and Western blot analysis | |
| 14 | Evaluation of Laboratory Notebooks for the Final Exam | Self-study |
| Resources |
| Laboratory Biosafety Manual (third edition) 1. Containment of biohazard - methods ISBN 92 4 154650 6 (LC/NLM classification: QY 25) WHO/ CDS/ CSR/ LYO/ 2004. 11 |
| Internet database |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Develops and deepens current and advanced knowledge in the field of histology with original thought and research at the level of expertise and reaches original definitions that will bring innovation to the field. | ||||||
| 2 | Defines cell and tissue at an advanced level (ultrastructural). | ||||||
| 3 | Knows the embryological details about the development of systems at an advanced level. | ||||||
| 4 | Comprehends the interdisciplinary interaction that the field is related to; reach original results by using knowledge that requires expertise in analyzing, synthesizing and evaluating new and complex ideas. | ||||||
| 5 | Develops a new idea, method, design or application that brings innovation to the field, or applies a known idea, method, design or application to a different field, researches, comprehends, designs, adapts and applies an original subject. | ||||||
| 6 | He/she writes the report of the research he/she participated in. It publishes in a national/internationally accepted peer-reviewed journal and presents it at scientific meetings. | ||||||
| 7 | It contributes to the progress in its field by independently carrying out an original work that brings innovations to its field, develops a new idea, method, design or application, or applies a known idea, method, design or application to a different field. | ||||||
| 8 | Develops new thoughts and methods related to the field by using high-level mental processes such as creative and critical thinking, problem solving and decision making. | ||||||
| 9 | Participates in meetings related to the field of histology, presents papers, participates in discussions, gives conferences, and publishes his studies in reputable journals specific to the field. | ||||||
| 10 | Collects, interprets, applies and announces data related to histology and embryology. | ||||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |