Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| ADVANCED HISTOCHEMICAL TECHNIQUES II | - | Fall Semester | 2+4 | 4 | 8 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Third Cycle (Doctorate Degree) |
| Course Type | Elective |
| Course Coordinator | Prof.Dr. İlknur KESKİN |
| Name of Lecturer(s) | Prof.Dr. İlknur KESKİN, Assoc.Prof. Muzaffer Başak ULKAY |
| Assistant(s) | |
| Aim | With advanced histochemical methods, the tissue specific proteins are displayed by microscope and to determine the tissue with protein isolation and quantitatively to assign tissue specific proteins. |
| Course Content | This course contains; Introduction to Histochemistry, Fixations and areas of use,Preparation of tissues and sectioning to analyze light in microscope,Application of immunohistochemistry protocol,Immunhistochemistry method,Application of Immunctochemistry Protocol ,Imaging and analysis of tissues prepared by immunohistochemical methods,Midterm,Application of immunohistochemistry method,Application of immunohistochemistry method,Western blot, general concepts, procedure and recognition of material used ,Western Blot Protein Isolation and Protein Quantity Determination,Western Blot, Electrophoresis, Transfer, Primer and Secondary Antibody Applications,Application of western blot method and Western blot analysis of results,Final exam. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| Defines histochemistry methods. | 14, 9 | A |
| Defines fixatives and their usage areas. | 14, 9 | A, E |
| Explains the steps of the immunohistochemistry protocol. | 14, 9 | A |
| Explains basic information about Western blot application. | 14, 9 | A |
| Explains the steps of preparing tissue for analysis under a light microscope. | 14, 6, 9 | A, E |
| It indicates the stages of sectioning from tissue. | 14, 17, 9 | A, E |
| Analyzes the data resulting from the practice of the immunohistochemistry protocol. | 14, 9 | A |
| Describes the Western blot method procedure and materials used. | 14, 9 | A |
| Determines the amount of protein after protein isolation. | 14, 6, 9 | A |
| Explains electrophoresis, transfer, primary and secondary antibody applications in the Western blot method. | 14, 9 | A |
| Analyzes Western Blot results. | 14, 9 | A |
| Teaching Methods: | 14: Self Study Method, 17: Experimental Technique, 6: Experiential Learning, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | Introduction to Histochemistry, Fixations and areas of use | Reading the relevant course presentation |
| 2 | Preparation of tissues and sectioning to analyze light in microscope | Reading the relevant course presentation |
| 3 | Application of immunohistochemistry protocol | Reading the relevant course presentation |
| 4 | Immunhistochemistry method | Reading the relevant course presentation |
| 5 | Application of Immunctochemistry Protocol | Reading the relevant course presentation |
| 6 | Imaging and analysis of tissues prepared by immunohistochemical methods | Reading the relevant course presentation |
| 7 | Midterm | - |
| 8 | Application of immunohistochemistry method | Reading the relevant course presentation |
| 9 | Application of immunohistochemistry method | Reading the relevant course presentation |
| 10 | Western blot, general concepts, procedure and recognition of material used | Reading the relevant course presentation |
| 11 | Western Blot Protein Isolation and Protein Quantity Determination | Reading the relevant course presentation |
| 12 | Western Blot, Electrophoresis, Transfer, Primer and Secondary Antibody Applications | Reading the relevant course presentation |
| 13 | Application of western blot method and Western blot analysis of results | Reading the relevant course presentation |
| 14 | Final exam | - |
| Resources |
| Gartner L.P, Hiatt J.L., Color Text Book of Histology, Second Edition, Saunders Company, Philadelphia, 1983. - Junqueıra L.C., Carneiro J. Basic Histology, Ankara, 2006. - Ross M.H., Pawlina W., Histology A Text and Atlas, Fourth Edition, Lippincott Williams & Wilkins Company, Baltimore, 2003. Lecture notes, www.abcam.com, www.ihcworld.com |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Develops and deepens current and advanced knowledge in the field of histology with original thought and research at the level of expertise and reaches original definitions that will bring innovation to the field. | X | |||||
| 2 | Defines cell and tissue at an advanced level (ultrastructural). | X | |||||
| 3 | Knows the embryological details about the development of systems at an advanced level. | ||||||
| 4 | Comprehends the interdisciplinary interaction that the field is related to; reach original results by using knowledge that requires expertise in analyzing, synthesizing and evaluating new and complex ideas. | ||||||
| 5 | Develops a new idea, method, design or application that brings innovation to the field, or applies a known idea, method, design or application to a different field, researches, comprehends, designs, adapts and applies an original subject. | X | |||||
| 6 | He/she writes the report of the research he/she participated in. It publishes in a national/internationally accepted peer-reviewed journal and presents it at scientific meetings. | X | |||||
| 7 | It contributes to the progress in its field by independently carrying out an original work that brings innovations to its field, develops a new idea, method, design or application, or applies a known idea, method, design or application to a different field. | X | |||||
| 8 | Develops new thoughts and methods related to the field by using high-level mental processes such as creative and critical thinking, problem solving and decision making. | X | |||||
| 9 | Participates in meetings related to the field of histology, presents papers, participates in discussions, gives conferences, and publishes his studies in reputable journals specific to the field. | ||||||
| 10 | Collects, interprets, applies and announces data related to histology and embryology. | X | |||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |
| ECTS / Workload Table | ||||||
| Activities | Number of | Duration(Hour) | Total Workload(Hour) | |||
| Course Hours | 14 | 5 | 70 | |||
| Guided Problem Solving | 14 | 8 | 112 | |||
| Resolution of Homework Problems and Submission as a Report | 3 | 6 | 18 | |||
| Term Project | 0 | 0 | 0 | |||
| Presentation of Project / Seminar | 0 | 0 | 0 | |||
| Quiz | 0 | 0 | 0 | |||
| Midterm Exam | 1 | 10 | 10 | |||
| General Exam | 1 | 15 | 15 | |||
| Performance Task, Maintenance Plan | 0 | 0 | 0 | |||
| Total Workload(Hour) | 225 | |||||
| Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(225/30) | 8 | |||||
| ECTS of the course: 30 hours of work is counted as 1 ECTS credit. | ||||||
Detail Informations of the Course
Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| ADVANCED HISTOCHEMICAL TECHNIQUES II | - | Fall Semester | 2+4 | 4 | 8 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Third Cycle (Doctorate Degree) |
| Course Type | Elective |
| Course Coordinator | Prof.Dr. İlknur KESKİN |
| Name of Lecturer(s) | Prof.Dr. İlknur KESKİN, Assoc.Prof. Muzaffer Başak ULKAY |
| Assistant(s) | |
| Aim | With advanced histochemical methods, the tissue specific proteins are displayed by microscope and to determine the tissue with protein isolation and quantitatively to assign tissue specific proteins. |
| Course Content | This course contains; Introduction to Histochemistry, Fixations and areas of use,Preparation of tissues and sectioning to analyze light in microscope,Application of immunohistochemistry protocol,Immunhistochemistry method,Application of Immunctochemistry Protocol ,Imaging and analysis of tissues prepared by immunohistochemical methods,Midterm,Application of immunohistochemistry method,Application of immunohistochemistry method,Western blot, general concepts, procedure and recognition of material used ,Western Blot Protein Isolation and Protein Quantity Determination,Western Blot, Electrophoresis, Transfer, Primer and Secondary Antibody Applications,Application of western blot method and Western blot analysis of results,Final exam. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| Defines histochemistry methods. | 14, 9 | A |
| Defines fixatives and their usage areas. | 14, 9 | A, E |
| Explains the steps of the immunohistochemistry protocol. | 14, 9 | A |
| Explains basic information about Western blot application. | 14, 9 | A |
| Explains the steps of preparing tissue for analysis under a light microscope. | 14, 6, 9 | A, E |
| It indicates the stages of sectioning from tissue. | 14, 17, 9 | A, E |
| Analyzes the data resulting from the practice of the immunohistochemistry protocol. | 14, 9 | A |
| Describes the Western blot method procedure and materials used. | 14, 9 | A |
| Determines the amount of protein after protein isolation. | 14, 6, 9 | A |
| Explains electrophoresis, transfer, primary and secondary antibody applications in the Western blot method. | 14, 9 | A |
| Analyzes Western Blot results. | 14, 9 | A |
| Teaching Methods: | 14: Self Study Method, 17: Experimental Technique, 6: Experiential Learning, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | Introduction to Histochemistry, Fixations and areas of use | Reading the relevant course presentation |
| 2 | Preparation of tissues and sectioning to analyze light in microscope | Reading the relevant course presentation |
| 3 | Application of immunohistochemistry protocol | Reading the relevant course presentation |
| 4 | Immunhistochemistry method | Reading the relevant course presentation |
| 5 | Application of Immunctochemistry Protocol | Reading the relevant course presentation |
| 6 | Imaging and analysis of tissues prepared by immunohistochemical methods | Reading the relevant course presentation |
| 7 | Midterm | - |
| 8 | Application of immunohistochemistry method | Reading the relevant course presentation |
| 9 | Application of immunohistochemistry method | Reading the relevant course presentation |
| 10 | Western blot, general concepts, procedure and recognition of material used | Reading the relevant course presentation |
| 11 | Western Blot Protein Isolation and Protein Quantity Determination | Reading the relevant course presentation |
| 12 | Western Blot, Electrophoresis, Transfer, Primer and Secondary Antibody Applications | Reading the relevant course presentation |
| 13 | Application of western blot method and Western blot analysis of results | Reading the relevant course presentation |
| 14 | Final exam | - |
| Resources |
| Gartner L.P, Hiatt J.L., Color Text Book of Histology, Second Edition, Saunders Company, Philadelphia, 1983. - Junqueıra L.C., Carneiro J. Basic Histology, Ankara, 2006. - Ross M.H., Pawlina W., Histology A Text and Atlas, Fourth Edition, Lippincott Williams & Wilkins Company, Baltimore, 2003. Lecture notes, www.abcam.com, www.ihcworld.com |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Develops and deepens current and advanced knowledge in the field of histology with original thought and research at the level of expertise and reaches original definitions that will bring innovation to the field. | X | |||||
| 2 | Defines cell and tissue at an advanced level (ultrastructural). | X | |||||
| 3 | Knows the embryological details about the development of systems at an advanced level. | ||||||
| 4 | Comprehends the interdisciplinary interaction that the field is related to; reach original results by using knowledge that requires expertise in analyzing, synthesizing and evaluating new and complex ideas. | ||||||
| 5 | Develops a new idea, method, design or application that brings innovation to the field, or applies a known idea, method, design or application to a different field, researches, comprehends, designs, adapts and applies an original subject. | X | |||||
| 6 | He/she writes the report of the research he/she participated in. It publishes in a national/internationally accepted peer-reviewed journal and presents it at scientific meetings. | X | |||||
| 7 | It contributes to the progress in its field by independently carrying out an original work that brings innovations to its field, develops a new idea, method, design or application, or applies a known idea, method, design or application to a different field. | X | |||||
| 8 | Develops new thoughts and methods related to the field by using high-level mental processes such as creative and critical thinking, problem solving and decision making. | X | |||||
| 9 | Participates in meetings related to the field of histology, presents papers, participates in discussions, gives conferences, and publishes his studies in reputable journals specific to the field. | ||||||
| 10 | Collects, interprets, applies and announces data related to histology and embryology. | X | |||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |