Course Supervisor: Prof. Dr Esra Çağavi, Course LaboAssistants: Laboratory supervisor from Research Institute for Health Sciences and Technologies (SABITA),
Aim
The aim of the “Laboratory Rotation-II” course is for Ph.D. level students to to explain the advanced technical approaches in laboratory application that is expected to incorporate in their thesis work understanding of the scope of different laboratory disciplines. By rotating in different labs every week, students are aimed to be exposed to the advanced molecular biology techniques, proteomics and genomics applications, FACS, primary and cell line culture applications, transgenic animal models, electrophysiology, neuroscience and cancer research applications, basic protein and gene expression analysis.
Course Content
This course contains; Electroencephalography (EEG) recording principles
,EEG application and signal analysis,Flourescence activated cell sorting (FACS) method and analysis ,Functional Neurology Applications,Flourescence activated cell sorting (FACS) method and analysis ,Experimental and transgenic animal models and applications, Evaluation of Laboratory Notebooks for the Midterm Exam,Cell survival tests analysis,Electrophysiology applications and analysis,Quantitative RTPCR analysis in Molecular Biology,Cancer Biology Lab and Applications,Immunohistochemistry and cryosectioning ,SDS-PAGE and Western blot analysis,Evaluation of Laboratory Notebooks for the Final Exam.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Explain the advanced culture methods, advanced approaches in molecular biology, experimental transgenic animal models and their applications, applications in neuroscience research, EEG principles and electrophsiology, fundamental approaches in cancer biology, cell survival analysis and FACS analysis.
14, 17, 3, 9
A, E
Explains advanced research techniques.
14, 17, 3, 9
A, E
Explain and provide solutions to various different experimental techniques.
14, 17, 3, 9
A, E
Define the principles of basic research laboratory equipments and can operate these devices.
14, 17, 3, 9
A, E
Explains the advanced data analysis approaches of scientific data.
14, 17, 3, 9
A, E
Teaching Methods:
14: Self Study Method, 17: Experimental Technique, 3: Problem Baded Learning Model, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, E: Homework
Course Outline
Order
Subjects
Preliminary Work
1
Electroencephalography (EEG) recording principles
Self-study
2
EEG application and signal analysis
3
Flourescence activated cell sorting (FACS) method and analysis
Self-study
4
Functional Neurology Applications
Self-study
5
Flourescence activated cell sorting (FACS) method and analysis
Self-study
6
Experimental and transgenic animal models and applications
Self-study
7
Evaluation of Laboratory Notebooks for the Midterm Exam
Self-study
8
Cell survival tests analysis
9
Electrophysiology applications and analysis
10
Quantitative RTPCR analysis in Molecular Biology
11
Cancer Biology Lab and Applications
12
Immunohistochemistry and cryosectioning
13
SDS-PAGE and Western blot analysis
14
Evaluation of Laboratory Notebooks for the Final Exam
Course Supervisor: Prof. Dr Esra Çağavi, Course LaboAssistants: Laboratory supervisor from Research Institute for Health Sciences and Technologies (SABITA),
Aim
The aim of the “Laboratory Rotation-II” course is for Ph.D. level students to to explain the advanced technical approaches in laboratory application that is expected to incorporate in their thesis work understanding of the scope of different laboratory disciplines. By rotating in different labs every week, students are aimed to be exposed to the advanced molecular biology techniques, proteomics and genomics applications, FACS, primary and cell line culture applications, transgenic animal models, electrophysiology, neuroscience and cancer research applications, basic protein and gene expression analysis.
Course Content
This course contains; Electroencephalography (EEG) recording principles
,EEG application and signal analysis,Flourescence activated cell sorting (FACS) method and analysis ,Functional Neurology Applications,Flourescence activated cell sorting (FACS) method and analysis ,Experimental and transgenic animal models and applications, Evaluation of Laboratory Notebooks for the Midterm Exam,Cell survival tests analysis,Electrophysiology applications and analysis,Quantitative RTPCR analysis in Molecular Biology,Cancer Biology Lab and Applications,Immunohistochemistry and cryosectioning ,SDS-PAGE and Western blot analysis,Evaluation of Laboratory Notebooks for the Final Exam.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Explain the advanced culture methods, advanced approaches in molecular biology, experimental transgenic animal models and their applications, applications in neuroscience research, EEG principles and electrophsiology, fundamental approaches in cancer biology, cell survival analysis and FACS analysis.
14, 17, 3, 9
A, E
Explains advanced research techniques.
14, 17, 3, 9
A, E
Explain and provide solutions to various different experimental techniques.
14, 17, 3, 9
A, E
Define the principles of basic research laboratory equipments and can operate these devices.
14, 17, 3, 9
A, E
Explains the advanced data analysis approaches of scientific data.
14, 17, 3, 9
A, E
Teaching Methods:
14: Self Study Method, 17: Experimental Technique, 3: Problem Baded Learning Model, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, E: Homework
Course Outline
Order
Subjects
Preliminary Work
1
Electroencephalography (EEG) recording principles
Self-study
2
EEG application and signal analysis
3
Flourescence activated cell sorting (FACS) method and analysis
Self-study
4
Functional Neurology Applications
Self-study
5
Flourescence activated cell sorting (FACS) method and analysis
Self-study
6
Experimental and transgenic animal models and applications
Self-study
7
Evaluation of Laboratory Notebooks for the Midterm Exam
Self-study
8
Cell survival tests analysis
9
Electrophysiology applications and analysis
10
Quantitative RTPCR analysis in Molecular Biology
11
Cancer Biology Lab and Applications
12
Immunohistochemistry and cryosectioning
13
SDS-PAGE and Western blot analysis
14
Evaluation of Laboratory Notebooks for the Final Exam