Ders Detayı
Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| ADVANCED APPLIED TECHNIQUES | - | Spring Semester | 1+2 | 2 | 6 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Third Cycle (Doctorate Degree) |
| Course Type | Elective |
| Course Coordinator | Assoc.Prof. Sultan Sibel ERDEM |
| Name of Lecturer(s) | Prof.Dr. Neslin EMEKLİ, Prof.Dr. Türkan YİĞİTBAŞI, Assoc.Prof. Sultan Sibel ERDEM, Assist.Prof. Gözde ÜLFER, Assist.Prof. Çağrı ÇAKICI, Assist.Prof. Derya CANSIZ |
| Assistant(s) | |
| Aim | To gain advanced knowledge about techniques and devices that are used for molecular analysis in biochemistry |
| Course Content | This course contains; The proteins in carbohydrate metabolism and their structure,The proteins in lipid metabolism and their structure,The proteins in protein synthesis and their importance,The proteins in nucleic acid metabolism and their structure,The proteins in coagulation and inflammation system,The proteins in arterial tissue,Signal transduction pathways,The proteins in cell membrane and cytosol,Posttranslational modifications,The characterization of protein complexes,Protein phosphorylation mechanisms,Proteomics research in diagnosis,Personalized medicine and proteomics,Bioinformatics and biochemistry. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| At the end of this course, the students will be able to: | ||
| 1-Explain the proteins in carbohydrate metabolism | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.2.Explains low density lipoproteins that are thought to prevent the development of atherosclerosis | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.3.Explains research on HDL using LC-MC techniques. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.4.Explains the research done with LC-MC techniques on LDL. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3-Explains the proteins in protein synthesis and their properties | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4-Explains proteins in nucleic acid metabolism and their properties | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5-Explains the proteins involved in the coagulation and inflammation system | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.3.Explains the obtaining of proteins that cause bleeding diathesis by chemical or biotechnological methods | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6-Explains the proteins in arterial tissue | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.3.Defines the importance of proteins released from endothelium in cardiovascular diseases | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.4.Explains the meaning of evaluating the proteins that play a role in these systems with two-dimensional electrophoresis, nano liquid chromatography, LC-MS/MS methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7-Explains signal transduction pathways | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.1.Explains the general properties of signal molecules | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.5.Explains endocrine, paracrine and autocrine effects | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8- Question the proteins in cell membrane and cytosol | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.1.Explains the functions of membrane proteins in recognition, signal transmission, transport and protection of cell structure. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.2.Explains the proteome of cytosol proteins that can change in various pathologies. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.3.Explains various proteomics approaches of cytosol and membrane proteins | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.4.Explains the membrane and cytosol proteins of the nucleus and other organelles of the cell | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.5.Explains posttranslational modifications of proteins in the membrane and cytosol. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 9-Discuss posttranslational modifications of the proteins | 10, 11, 16, 19, 6, 9 | A, D, E |
| 10- Explain the characterization of protein complexes | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.1. Describe the proteins in glycogen synthesis and degradation | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.2. Describe the proteins in glycolysis pathway | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.3. Describe the proteins in pentose-phosphate pathway | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.4. Explains the proteins in fructose and galactose metabolic pathway | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.5. Explains the proteins in galactose and galactose metabolic pathway | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.1. Describe high and low-density lipoproteins that are related to atherosclerosis | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.1. Explains high and low-density lipoproteins that are related to atherosclerosis | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.5. Explain lipoproteins, which are active in lipid metabolism, for treatment purposes. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.1. Explains the role of coagulation proteins in thrombus development and bleeding diathesis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.2. Explains the plasma proteins that are effective in thrombus development. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.4. Explains the mechanism of action of these proteins | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.1. Understands the mechanism that causes cardiovascular, cerebrovascular and peripheral vascular diseases. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.2. Describe the importance of plasma proteins in cardiovascular diseases | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.5. Explains the mechanism underlying atherosclerosis | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.2. Explains target cell receptors, cellular response, properties of neurotransmitters | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.3. Explain the properties of hormones in the endocrine system, cytokines and other signaling molecules in the immune system. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.4. Explains the relationship between receptor and target cell response. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.6. Describes important plasma membrane receptors | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.7. Explains various pathways in the target cell response such as tyrosine kinase receptors, RAS and eMAP kinase pathway, phosphotidyl inositol phosphate, insulin receptor, JAK-STAT receptors, serine-threonine kinase, hepahelical receptors… | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.6. Explanes the analysis and identification of membrane and cytosolic proteins by LC-MS/MS | 10, 11, 16, 19, 6, 9 | A, D, E |
| Teaching Methods: | 10: Discussion Method, 11: Demonstration Method, 16: Question - Answer Technique, 19: Brainstorming Technique, 6: Experiential Learning, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, D: Oral Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | The proteins in carbohydrate metabolism and their structure | 1, 2, 3, 4, 5 |
| 2 | The proteins in lipid metabolism and their structure | 1, 2, 3, 4, 5 |
| 3 | The proteins in protein synthesis and their importance | 1, 2, 3, 4, 5 |
| 4 | The proteins in nucleic acid metabolism and their structure | 1, 2, 3, 4, 5 |
| 5 | The proteins in coagulation and inflammation system | 1, 2, 3, 4, 5 |
| 6 | The proteins in arterial tissue | 1, 2, 3, 4, 5 |
| 7 | Signal transduction pathways | 1, 2, 3, 4, 5 |
| 8 | The proteins in cell membrane and cytosol | 1, 2, 3, 4, 5 |
| 9 | Posttranslational modifications | 1, 2, 3, 4, 5 |
| 10 | The characterization of protein complexes | 1, 2, 3, 4, 5 |
| 11 | Protein phosphorylation mechanisms | 1, 2, 3, 4, 5 |
| 12 | Proteomics research in diagnosis | 1, 2, 3, 4, 5 |
| 13 | Personalized medicine and proteomics | 1, 2, 3, 4, 5 |
| 14 | Bioinformatics and biochemistry | 1, 2, 3, 4, 5 |
| Resources |
| Published books of Prof.Dr.Nesrin Emekli and Assoc.Prof.Dr.Türkan Yiğitbaşı |
| Prof.Dr. Nesrin Emekli, Doç.Dr.Türkan Yiğitbaşı (Ed.). Klinik Biyokimya Biyokimyada İleri Araştırma Teknikleri (Section 6) Moleküler Tanı Teknikleri (Section 7) |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Defines macro and micromolecules in cell and body fluids at an advanced level. | ||||||
| 2 | Knows how to store and protect the material to be analyzed. | ||||||
| 3 | Knows how to identify molecules found in cells and body fluids. | ||||||
| 4 | Knows the principles of technologies to be used for routine and research purposes. | ||||||
| 5 | Knows how to evaluate the results of the study, interprets and questions. | ||||||
| 6 | Knows how to use field-specific tools and equipment | ||||||
| 7 | Knows how to access and interpret field-specific resources and information | ||||||
| 8 | Shares information with disciplines related to the field | ||||||
| 9 | Combines theoretical and factual knowledge with critical thoughts, conducts independent studies, evaluates the results, compares them with the literature, and gets them accepted by authors and scientific journals. | ||||||
| 10 | Solves a scientific and technical problem, knows where to get help. | ||||||
| 11 | Takes responsibility in joint scientific studies, contributes and works in harmony. | ||||||
| 12 | Participates in meetings related to the field of biochemistry, presents papers, participates in discussions and congresses, publishes studies in reputable journals specific to the field. | ||||||
| 13 | Collects, interprets, applies, announces data related to the field of biochemistry, conducts social and cultural studies, and supervises these studies scientifically and socially. | ||||||
| 14 | Can take part in management activities, evaluate the results obtained to improve quality | ||||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |
| ECTS / Workload Table | ||||||
| Activities | Number of | Duration(Hour) | Total Workload(Hour) | |||
| Course Hours | 14 | 1 | 14 | |||
| Guided Problem Solving | 14 | 11 | 154 | |||
| Resolution of Homework Problems and Submission as a Report | 0 | 0 | 0 | |||
| Term Project | 2 | 4 | 8 | |||
| Presentation of Project / Seminar | 2 | 3 | 6 | |||
| Quiz | 0 | 0 | 0 | |||
| Midterm Exam | 1 | 3 | 3 | |||
| General Exam | 1 | 4 | 4 | |||
| Performance Task, Maintenance Plan | 0 | 0 | 0 | |||
| Total Workload(Hour) | 189 | |||||
| Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(189/30) | 6 | |||||
| ECTS of the course: 30 hours of work is counted as 1 ECTS credit. | ||||||
Detail Informations of the Course
Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| ADVANCED APPLIED TECHNIQUES | - | Spring Semester | 1+2 | 2 | 6 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Third Cycle (Doctorate Degree) |
| Course Type | Elective |
| Course Coordinator | Assoc.Prof. Sultan Sibel ERDEM |
| Name of Lecturer(s) | Prof.Dr. Neslin EMEKLİ, Prof.Dr. Türkan YİĞİTBAŞI, Assoc.Prof. Sultan Sibel ERDEM, Assist.Prof. Gözde ÜLFER, Assist.Prof. Çağrı ÇAKICI, Assist.Prof. Derya CANSIZ |
| Assistant(s) | |
| Aim | To gain advanced knowledge about techniques and devices that are used for molecular analysis in biochemistry |
| Course Content | This course contains; The proteins in carbohydrate metabolism and their structure,The proteins in lipid metabolism and their structure,The proteins in protein synthesis and their importance,The proteins in nucleic acid metabolism and their structure,The proteins in coagulation and inflammation system,The proteins in arterial tissue,Signal transduction pathways,The proteins in cell membrane and cytosol,Posttranslational modifications,The characterization of protein complexes,Protein phosphorylation mechanisms,Proteomics research in diagnosis,Personalized medicine and proteomics,Bioinformatics and biochemistry. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| At the end of this course, the students will be able to: | ||
| 1-Explain the proteins in carbohydrate metabolism | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.2.Explains low density lipoproteins that are thought to prevent the development of atherosclerosis | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.3.Explains research on HDL using LC-MC techniques. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.4.Explains the research done with LC-MC techniques on LDL. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3-Explains the proteins in protein synthesis and their properties | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4-Explains proteins in nucleic acid metabolism and their properties | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5-Explains the proteins involved in the coagulation and inflammation system | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.3.Explains the obtaining of proteins that cause bleeding diathesis by chemical or biotechnological methods | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6-Explains the proteins in arterial tissue | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.3.Defines the importance of proteins released from endothelium in cardiovascular diseases | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.4.Explains the meaning of evaluating the proteins that play a role in these systems with two-dimensional electrophoresis, nano liquid chromatography, LC-MS/MS methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7-Explains signal transduction pathways | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.1.Explains the general properties of signal molecules | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.5.Explains endocrine, paracrine and autocrine effects | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8- Question the proteins in cell membrane and cytosol | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.1.Explains the functions of membrane proteins in recognition, signal transmission, transport and protection of cell structure. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.2.Explains the proteome of cytosol proteins that can change in various pathologies. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.3.Explains various proteomics approaches of cytosol and membrane proteins | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.4.Explains the membrane and cytosol proteins of the nucleus and other organelles of the cell | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.5.Explains posttranslational modifications of proteins in the membrane and cytosol. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 9-Discuss posttranslational modifications of the proteins | 10, 11, 16, 19, 6, 9 | A, D, E |
| 10- Explain the characterization of protein complexes | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.1. Describe the proteins in glycogen synthesis and degradation | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.2. Describe the proteins in glycolysis pathway | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.3. Describe the proteins in pentose-phosphate pathway | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.4. Explains the proteins in fructose and galactose metabolic pathway | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.5. Explains the proteins in galactose and galactose metabolic pathway | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.1. Describe high and low-density lipoproteins that are related to atherosclerosis | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.1. Explains high and low-density lipoproteins that are related to atherosclerosis | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.5. Explain lipoproteins, which are active in lipid metabolism, for treatment purposes. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.1. Explains the role of coagulation proteins in thrombus development and bleeding diathesis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.2. Explains the plasma proteins that are effective in thrombus development. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.4. Explains the mechanism of action of these proteins | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.1. Understands the mechanism that causes cardiovascular, cerebrovascular and peripheral vascular diseases. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.2. Describe the importance of plasma proteins in cardiovascular diseases | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.5. Explains the mechanism underlying atherosclerosis | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.2. Explains target cell receptors, cellular response, properties of neurotransmitters | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.3. Explain the properties of hormones in the endocrine system, cytokines and other signaling molecules in the immune system. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.4. Explains the relationship between receptor and target cell response. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.6. Describes important plasma membrane receptors | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.7. Explains various pathways in the target cell response such as tyrosine kinase receptors, RAS and eMAP kinase pathway, phosphotidyl inositol phosphate, insulin receptor, JAK-STAT receptors, serine-threonine kinase, hepahelical receptors… | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.6. Explanes the analysis and identification of membrane and cytosolic proteins by LC-MS/MS | 10, 11, 16, 19, 6, 9 | A, D, E |
| Teaching Methods: | 10: Discussion Method, 11: Demonstration Method, 16: Question - Answer Technique, 19: Brainstorming Technique, 6: Experiential Learning, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, D: Oral Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | The proteins in carbohydrate metabolism and their structure | 1, 2, 3, 4, 5 |
| 2 | The proteins in lipid metabolism and their structure | 1, 2, 3, 4, 5 |
| 3 | The proteins in protein synthesis and their importance | 1, 2, 3, 4, 5 |
| 4 | The proteins in nucleic acid metabolism and their structure | 1, 2, 3, 4, 5 |
| 5 | The proteins in coagulation and inflammation system | 1, 2, 3, 4, 5 |
| 6 | The proteins in arterial tissue | 1, 2, 3, 4, 5 |
| 7 | Signal transduction pathways | 1, 2, 3, 4, 5 |
| 8 | The proteins in cell membrane and cytosol | 1, 2, 3, 4, 5 |
| 9 | Posttranslational modifications | 1, 2, 3, 4, 5 |
| 10 | The characterization of protein complexes | 1, 2, 3, 4, 5 |
| 11 | Protein phosphorylation mechanisms | 1, 2, 3, 4, 5 |
| 12 | Proteomics research in diagnosis | 1, 2, 3, 4, 5 |
| 13 | Personalized medicine and proteomics | 1, 2, 3, 4, 5 |
| 14 | Bioinformatics and biochemistry | 1, 2, 3, 4, 5 |
| Resources |
| Published books of Prof.Dr.Nesrin Emekli and Assoc.Prof.Dr.Türkan Yiğitbaşı |
| Prof.Dr. Nesrin Emekli, Doç.Dr.Türkan Yiğitbaşı (Ed.). Klinik Biyokimya Biyokimyada İleri Araştırma Teknikleri (Section 6) Moleküler Tanı Teknikleri (Section 7) |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Defines macro and micromolecules in cell and body fluids at an advanced level. | ||||||
| 2 | Knows how to store and protect the material to be analyzed. | ||||||
| 3 | Knows how to identify molecules found in cells and body fluids. | ||||||
| 4 | Knows the principles of technologies to be used for routine and research purposes. | ||||||
| 5 | Knows how to evaluate the results of the study, interprets and questions. | ||||||
| 6 | Knows how to use field-specific tools and equipment | ||||||
| 7 | Knows how to access and interpret field-specific resources and information | ||||||
| 8 | Shares information with disciplines related to the field | ||||||
| 9 | Combines theoretical and factual knowledge with critical thoughts, conducts independent studies, evaluates the results, compares them with the literature, and gets them accepted by authors and scientific journals. | ||||||
| 10 | Solves a scientific and technical problem, knows where to get help. | ||||||
| 11 | Takes responsibility in joint scientific studies, contributes and works in harmony. | ||||||
| 12 | Participates in meetings related to the field of biochemistry, presents papers, participates in discussions and congresses, publishes studies in reputable journals specific to the field. | ||||||
| 13 | Collects, interprets, applies, announces data related to the field of biochemistry, conducts social and cultural studies, and supervises these studies scientifically and socially. | ||||||
| 14 | Can take part in management activities, evaluate the results obtained to improve quality | ||||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |