Ders Detayı
Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| ADVANCED BIOCHEMISTRY II | - | Spring Semester | 2+0 | 2 | 6 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Third Cycle (Doctorate Degree) |
| Course Type | Elective |
| Course Coordinator | |
| Name of Lecturer(s) | Prof.Dr. Neslin EMEKLİ, Prof.Dr. Türkan YİĞİTBAŞI, Assoc.Prof. Sultan Sibel ERDEM, Assist.Prof. Gözde ÜLFER, Assist.Prof. Çağrı ÇAKICI, Assist.Prof. Derya CANSIZ |
| Assistant(s) | |
| Aim | To teach advanced level understanding of biochemical concepts in human body |
| Course Content | This course contains; Biomembranes and signal transduction,Biochemistry of connective tissue,Biochemistry of muscle,Inflamation and cytokines,Calcium and phosphate biochemistry,Apoptosis,Nerve system and related biochemical reactions,Fluid and electrolyte biochemistry,Iron metabolism,Non enzymatic glycosylation (NEG),Lipoproteins and atherosclerosis,New cardiovascular markers,Tissue factor and tissue factor pathway inhibitor,Saliva as a diagnostic material. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| At the end of this course the student will be able to: 1-Can question the proteins in carbohydrate metabolism. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.4.Defines the proteins and pathologies in the metabolic pathway of fructose. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.5.Defines the proteins in the metabolic pathway of galactose and their pathologies. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2- Can question the proteins in lipid metabolism. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.2.Explains low density lipoproteins that are thought to prevent the development of atherosclerosis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.3.questions the researches on HDL with LC-MC techniques. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.4.questions the researches on LDL with LC-MC techniques. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3-Explains proteins in protein synthesis and their properties. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4-Explains proteins in nucleic acid metabolism and their properties. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5-Interrogate the proteins involved in coagulation and inflammation system. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.3.Analyses the proteins causing bleeding diathesis by chemical or biotechnological methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6-Can question the proteins in arterial tissue. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.3.Defines the importance of proteins released from the endothelium in cardiovascular diseases. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.4.Questions the meaning of evaluating the proteins involved in these systems by two dimensional electrophoresis, nano liquid chromatography, nLC-MS / MS methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7-Can interrogate the signal transmission. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.1.Explains the general properties of signalling molecules. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.5.Defines endocrine, paracrine and autocrine effects. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8- Can question the proteins in the cell membrane and cytosol. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.1.Defines the functions of membrane proteins recognition, signal transduction, transport, protection of cell structure. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.2.Explains the proteome of cytosol proteins that may vary in various pathologies. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.3.Interrogates various proteomics approaches of cytosol and membrane proteins. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.4.Explains the membrane and cytosol proteins of the nucleus and other organelles of the cell. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.5.Questions the posttranslational modifications of proteins in membrane and cytosol. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 9- Questions the posttranslational modification of proteins. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 10- Can question the characterisation of protein complexes. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 11- Questions the phosphorylation mechanism of proteins. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 12-Questions the importance of proteomics in terms of diagnosis and treatment. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 13-Interrogates the meaning of proteomics in personal medicine. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 14-Questions the importance of bioinformatics in biochemistry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.1. Defines the proteins and pathologies in glycogen production and degradation. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.2. Defines the properties of proteins in the glycolysis pathway and haemolytic anaemias. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.3. Defines the properties and pathologies of proteins in the pentose phosphate pathway. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.1. Explains high density lipoprotenes which are thought to prevent the development of atherosclerosis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.5. Question these lipoproteins which are active in lipid metabolism for treatment. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.1. Explains the role of coagulation proteins in thrombus development and bleeding diathesis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.2. Questions the plasma proteins that are effective in thrombus development. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.4. Explains the mechanism of action of these proteins. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.1. Explains the mechanism that causes cardiovascular, cerebrovascular and peripheral vascular diseases. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.2. Defines the importance of plasma proteins in cardiovascular diseases. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.5. Question the underlying mechanism of atherosclerosis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.2. Defines target cell receptors, cellular response, properties of neurotransmitters. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.3. Describes the properties of hormones in the endocrine system, cytokines and other signalling molecules in the immune system. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.4. Questions the relationship between receptor and target cell response. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.6. Defines important plasma membrane receptors. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.7. Describe various pathways in target cell response such as tyrosine kinase receptors, RAS and eMAP kinase pathway, phosphotidyl inositol phosphate, insulin receptor, JAK-STAT receptors, serine-threonine kinase, hepahelical receptors. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.6. Analyses and identifies membrane and cytosolic proteins by LC-MS/MS. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 9.1. Explains different posttrasnlational modifications. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 9.2. Explains the mechanisms of intracellular posttranslational modification. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 9.3. Question the purpose of posttranslational modifications and their effect on protein. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 9.4. Explains the qualitative and quantitative analysis of these modifications. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 9.5. questions the intracellular effect of posttranslational modifications. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 10.1. Explains the mechanisms of formation of protein complexes. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 10.2. Analyses protein-protein interactions. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 10.3. Explains the analytical methods required for protein analyses. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 10.4. Explains the kinetics of protein-protein interaction. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 10.5. Explains advanced methods in the analysis of protein complexes. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 11.1. Questions the biological meaning of phosphorylation. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 11.2. Explains protein phosphorylation mechanisms. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 11.3. Demonstrate how phosphorylations can be analysed. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 11.4. Analyses intracellular signal transduction. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 11.5. Describes the software and hardware required for analysis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 12.1. Defines biomarker discovery. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 12.2. Explains the place of proteomics in disease mechanisms. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 12.3. Explains sample preparation and analysis methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 12.4. Explains the importance of statistics in biomarker discovery. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 12.5. Explains the limits and shortcomings of existing technology. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 13.1. explains the place of proteomics in personalised drug development. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 13.2. Explains different methods of analysis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 13.3. Shows the necessary experimental method and interpretation according to the disease. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 13.4. Explains the potential of proteomics in personal medicine. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 13.5. Explains how to make sense of data. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 14.1. Questions complex pathway analyses. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 14.2. Demonstrates different omics approaches and using them together. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 14.3. Questions the information that bioinformatics can provide. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 14.4. Analyses at molecular level. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 14.5. Explains the methods and software used in bioinformatics. | 10, 11, 16, 19, 6, 9 | A, D, E |
| Teaching Methods: | 10: Discussion Method, 11: Demonstration Method, 16: Question - Answer Technique, 19: Brainstorming Technique, 6: Experiential Learning, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, D: Oral Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | Biomembranes and signal transduction | 1, 2, 3, 4, 5, 6 |
| 2 | Biochemistry of connective tissue | 1, 2, 3, 4, 5, 6 |
| 3 | Biochemistry of muscle | 1, 2, 3, 4, 5, 6 |
| 4 | Inflamation and cytokines | 1, 2, 3, 4, 5, 6 |
| 5 | Calcium and phosphate biochemistry | 1, 2, 3, 4, 5, 6 |
| 6 | Apoptosis | 1, 2, 3, 4, 5, 6 |
| 7 | Nerve system and related biochemical reactions | 1, 2, 3, 4, 5, 6 |
| 8 | Fluid and electrolyte biochemistry | 1, 2, 3, 4, 5, 6 |
| 9 | Iron metabolism | 1, 2, 3, 4, 5, 6 |
| 10 | Non enzymatic glycosylation (NEG) | 1, 2, 3, 4, 5, 6 |
| 11 | Lipoproteins and atherosclerosis | 1, 2, 3, 4, 5, 6 |
| 12 | New cardiovascular markers | 1, 2, 3, 4, 5, 6 |
| 13 | Tissue factor and tissue factor pathway inhibitor | 1, 2, 3, 4, 5, 6 |
| 14 | Saliva as a diagnostic material | 1, 2, 3, 4, 5, 6 |
| Resources |
| 1) Vascular Proteomics/Methods and protokols Ed.Fernando Vivanco Humana Pres 2013 2) Timothy D. Veenstra. Proteomic Applications in Cancer Detection and Discovery, Wiley&Sons Inc,New Jersey, 2013 3) Lieberman M, Marks AD. Marks’ Basic medical biochemistry 3. Baskı, Lippincott Williams&Wilkins, 2010. 4) Bishop ML, Fody EP,Schoeff LE. Clinical Chemistry 7.Baskı,s.323-334. Lippincott, 2013. 5) Henry’s Clinical Diagnosisand Management by Laboratory Method,.22.Baskı,Eds.Pherson RA, Pincus MR,Elsevier-Saunders, 2011. 6) Harper Biochemistry (Last Edition) |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Defines macro and micromolecules in cell and body fluids at an advanced level. | X | |||||
| 2 | Knows how to store and protect the material to be analyzed. | X | |||||
| 3 | Knows how to identify molecules found in cells and body fluids. | X | |||||
| 4 | Knows the principles of technologies to be used for routine and research purposes. | X | |||||
| 5 | Knows how to evaluate the results of the study, interprets and questions. | X | |||||
| 6 | Knows how to use field-specific tools and equipment | X | |||||
| 7 | Knows how to access and interpret field-specific resources and information | X | |||||
| 8 | Shares information with disciplines related to the field | X | |||||
| 9 | Combines theoretical and factual knowledge with critical thoughts, conducts independent studies, evaluates the results, compares them with the literature, and gets them accepted by authors and scientific journals. | X | |||||
| 10 | Solves a scientific and technical problem, knows where to get help. | X | |||||
| 11 | Takes responsibility in joint scientific studies, contributes and works in harmony. | X | |||||
| 12 | Participates in meetings related to the field of biochemistry, presents papers, participates in discussions and congresses, publishes studies in reputable journals specific to the field. | X | |||||
| 13 | Collects, interprets, applies, announces data related to the field of biochemistry, conducts social and cultural studies, and supervises these studies scientifically and socially. | X | |||||
| 14 | Can take part in management activities, evaluate the results obtained to improve quality | X | |||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |
| ECTS / Workload Table | ||||||
| Activities | Number of | Duration(Hour) | Total Workload(Hour) | |||
| Course Hours | 14 | 2 | 28 | |||
| Guided Problem Solving | 14 | 4 | 56 | |||
| Resolution of Homework Problems and Submission as a Report | 0 | 0 | 0 | |||
| Term Project | 2 | 5 | 10 | |||
| Presentation of Project / Seminar | 2 | 4 | 8 | |||
| Quiz | 0 | 0 | 0 | |||
| Midterm Exam | 1 | 30 | 30 | |||
| General Exam | 1 | 35 | 35 | |||
| Performance Task, Maintenance Plan | 0 | 0 | 0 | |||
| Total Workload(Hour) | 167 | |||||
| Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(167/30) | 6 | |||||
| ECTS of the course: 30 hours of work is counted as 1 ECTS credit. | ||||||
Detail Informations of the Course
Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| ADVANCED BIOCHEMISTRY II | - | Spring Semester | 2+0 | 2 | 6 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Third Cycle (Doctorate Degree) |
| Course Type | Elective |
| Course Coordinator | |
| Name of Lecturer(s) | Prof.Dr. Neslin EMEKLİ, Prof.Dr. Türkan YİĞİTBAŞI, Assoc.Prof. Sultan Sibel ERDEM, Assist.Prof. Gözde ÜLFER, Assist.Prof. Çağrı ÇAKICI, Assist.Prof. Derya CANSIZ |
| Assistant(s) | |
| Aim | To teach advanced level understanding of biochemical concepts in human body |
| Course Content | This course contains; Biomembranes and signal transduction,Biochemistry of connective tissue,Biochemistry of muscle,Inflamation and cytokines,Calcium and phosphate biochemistry,Apoptosis,Nerve system and related biochemical reactions,Fluid and electrolyte biochemistry,Iron metabolism,Non enzymatic glycosylation (NEG),Lipoproteins and atherosclerosis,New cardiovascular markers,Tissue factor and tissue factor pathway inhibitor,Saliva as a diagnostic material. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| At the end of this course the student will be able to: 1-Can question the proteins in carbohydrate metabolism. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.4.Defines the proteins and pathologies in the metabolic pathway of fructose. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.5.Defines the proteins in the metabolic pathway of galactose and their pathologies. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2- Can question the proteins in lipid metabolism. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.2.Explains low density lipoproteins that are thought to prevent the development of atherosclerosis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.3.questions the researches on HDL with LC-MC techniques. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.4.questions the researches on LDL with LC-MC techniques. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3-Explains proteins in protein synthesis and their properties. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4-Explains proteins in nucleic acid metabolism and their properties. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5-Interrogate the proteins involved in coagulation and inflammation system. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.3.Analyses the proteins causing bleeding diathesis by chemical or biotechnological methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6-Can question the proteins in arterial tissue. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.3.Defines the importance of proteins released from the endothelium in cardiovascular diseases. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.4.Questions the meaning of evaluating the proteins involved in these systems by two dimensional electrophoresis, nano liquid chromatography, nLC-MS / MS methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7-Can interrogate the signal transmission. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.1.Explains the general properties of signalling molecules. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.5.Defines endocrine, paracrine and autocrine effects. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8- Can question the proteins in the cell membrane and cytosol. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.1.Defines the functions of membrane proteins recognition, signal transduction, transport, protection of cell structure. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.2.Explains the proteome of cytosol proteins that may vary in various pathologies. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.3.Interrogates various proteomics approaches of cytosol and membrane proteins. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.4.Explains the membrane and cytosol proteins of the nucleus and other organelles of the cell. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.5.Questions the posttranslational modifications of proteins in membrane and cytosol. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 9- Questions the posttranslational modification of proteins. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 10- Can question the characterisation of protein complexes. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 11- Questions the phosphorylation mechanism of proteins. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 12-Questions the importance of proteomics in terms of diagnosis and treatment. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 13-Interrogates the meaning of proteomics in personal medicine. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 14-Questions the importance of bioinformatics in biochemistry. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.1. Defines the proteins and pathologies in glycogen production and degradation. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.2. Defines the properties of proteins in the glycolysis pathway and haemolytic anaemias. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.3. Defines the properties and pathologies of proteins in the pentose phosphate pathway. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.1. Explains high density lipoprotenes which are thought to prevent the development of atherosclerosis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.5. Question these lipoproteins which are active in lipid metabolism for treatment. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.1. Explains the role of coagulation proteins in thrombus development and bleeding diathesis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.2. Questions the plasma proteins that are effective in thrombus development. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.4. Explains the mechanism of action of these proteins. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.1. Explains the mechanism that causes cardiovascular, cerebrovascular and peripheral vascular diseases. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.2. Defines the importance of plasma proteins in cardiovascular diseases. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 6.5. Question the underlying mechanism of atherosclerosis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.2. Defines target cell receptors, cellular response, properties of neurotransmitters. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.3. Describes the properties of hormones in the endocrine system, cytokines and other signalling molecules in the immune system. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.4. Questions the relationship between receptor and target cell response. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.6. Defines important plasma membrane receptors. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 7.7. Describe various pathways in target cell response such as tyrosine kinase receptors, RAS and eMAP kinase pathway, phosphotidyl inositol phosphate, insulin receptor, JAK-STAT receptors, serine-threonine kinase, hepahelical receptors. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 8.6. Analyses and identifies membrane and cytosolic proteins by LC-MS/MS. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 9.1. Explains different posttrasnlational modifications. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 9.2. Explains the mechanisms of intracellular posttranslational modification. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 9.3. Question the purpose of posttranslational modifications and their effect on protein. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 9.4. Explains the qualitative and quantitative analysis of these modifications. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 9.5. questions the intracellular effect of posttranslational modifications. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 10.1. Explains the mechanisms of formation of protein complexes. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 10.2. Analyses protein-protein interactions. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 10.3. Explains the analytical methods required for protein analyses. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 10.4. Explains the kinetics of protein-protein interaction. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 10.5. Explains advanced methods in the analysis of protein complexes. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 11.1. Questions the biological meaning of phosphorylation. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 11.2. Explains protein phosphorylation mechanisms. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 11.3. Demonstrate how phosphorylations can be analysed. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 11.4. Analyses intracellular signal transduction. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 11.5. Describes the software and hardware required for analysis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 12.1. Defines biomarker discovery. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 12.2. Explains the place of proteomics in disease mechanisms. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 12.3. Explains sample preparation and analysis methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 12.4. Explains the importance of statistics in biomarker discovery. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 12.5. Explains the limits and shortcomings of existing technology. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 13.1. explains the place of proteomics in personalised drug development. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 13.2. Explains different methods of analysis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 13.3. Shows the necessary experimental method and interpretation according to the disease. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 13.4. Explains the potential of proteomics in personal medicine. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 13.5. Explains how to make sense of data. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 14.1. Questions complex pathway analyses. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 14.2. Demonstrates different omics approaches and using them together. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 14.3. Questions the information that bioinformatics can provide. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 14.4. Analyses at molecular level. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 14.5. Explains the methods and software used in bioinformatics. | 10, 11, 16, 19, 6, 9 | A, D, E |
| Teaching Methods: | 10: Discussion Method, 11: Demonstration Method, 16: Question - Answer Technique, 19: Brainstorming Technique, 6: Experiential Learning, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, D: Oral Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | Biomembranes and signal transduction | 1, 2, 3, 4, 5, 6 |
| 2 | Biochemistry of connective tissue | 1, 2, 3, 4, 5, 6 |
| 3 | Biochemistry of muscle | 1, 2, 3, 4, 5, 6 |
| 4 | Inflamation and cytokines | 1, 2, 3, 4, 5, 6 |
| 5 | Calcium and phosphate biochemistry | 1, 2, 3, 4, 5, 6 |
| 6 | Apoptosis | 1, 2, 3, 4, 5, 6 |
| 7 | Nerve system and related biochemical reactions | 1, 2, 3, 4, 5, 6 |
| 8 | Fluid and electrolyte biochemistry | 1, 2, 3, 4, 5, 6 |
| 9 | Iron metabolism | 1, 2, 3, 4, 5, 6 |
| 10 | Non enzymatic glycosylation (NEG) | 1, 2, 3, 4, 5, 6 |
| 11 | Lipoproteins and atherosclerosis | 1, 2, 3, 4, 5, 6 |
| 12 | New cardiovascular markers | 1, 2, 3, 4, 5, 6 |
| 13 | Tissue factor and tissue factor pathway inhibitor | 1, 2, 3, 4, 5, 6 |
| 14 | Saliva as a diagnostic material | 1, 2, 3, 4, 5, 6 |
| Resources |
| 1) Vascular Proteomics/Methods and protokols Ed.Fernando Vivanco Humana Pres 2013 2) Timothy D. Veenstra. Proteomic Applications in Cancer Detection and Discovery, Wiley&Sons Inc,New Jersey, 2013 3) Lieberman M, Marks AD. Marks’ Basic medical biochemistry 3. Baskı, Lippincott Williams&Wilkins, 2010. 4) Bishop ML, Fody EP,Schoeff LE. Clinical Chemistry 7.Baskı,s.323-334. Lippincott, 2013. 5) Henry’s Clinical Diagnosisand Management by Laboratory Method,.22.Baskı,Eds.Pherson RA, Pincus MR,Elsevier-Saunders, 2011. 6) Harper Biochemistry (Last Edition) |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Defines macro and micromolecules in cell and body fluids at an advanced level. | X | |||||
| 2 | Knows how to store and protect the material to be analyzed. | X | |||||
| 3 | Knows how to identify molecules found in cells and body fluids. | X | |||||
| 4 | Knows the principles of technologies to be used for routine and research purposes. | X | |||||
| 5 | Knows how to evaluate the results of the study, interprets and questions. | X | |||||
| 6 | Knows how to use field-specific tools and equipment | X | |||||
| 7 | Knows how to access and interpret field-specific resources and information | X | |||||
| 8 | Shares information with disciplines related to the field | X | |||||
| 9 | Combines theoretical and factual knowledge with critical thoughts, conducts independent studies, evaluates the results, compares them with the literature, and gets them accepted by authors and scientific journals. | X | |||||
| 10 | Solves a scientific and technical problem, knows where to get help. | X | |||||
| 11 | Takes responsibility in joint scientific studies, contributes and works in harmony. | X | |||||
| 12 | Participates in meetings related to the field of biochemistry, presents papers, participates in discussions and congresses, publishes studies in reputable journals specific to the field. | X | |||||
| 13 | Collects, interprets, applies, announces data related to the field of biochemistry, conducts social and cultural studies, and supervises these studies scientifically and socially. | X | |||||
| 14 | Can take part in management activities, evaluate the results obtained to improve quality | X | |||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |