Course Supervisor: Prof. Dr Esra Çağavi,
Course LaboAssistants: Laboratory supervisor from Research Institute for Health Sciences and Technologies (SABITA),
Aim
The aim of the “Laboratory Rotation-I” course is for Ph.D. level students to explain the advanced technical approaches in scientific research, the basic laboratory equipment usage instructions, and laboratory application that will be incorporated to the thesis work by understanding the scope of different laboratory disciplines. By rotating in different labs every week, students are aimed to explain laboratory safety and instructions and practice advanced molecular biology techniques, proteomics and genomics applications, flow cytometry and analysis, histology applications, microscopy imaging systems and image analysis, cell culture and experimental animal laboratories.
Course Content
This course contains; Advanced Molecular Biology Techniques-1, Lab-1,Advanced Molecular Biology Techniques-2, Lab-2,Electrophysiology laboratory,Advanced Molecular Biology Techniques-3, Lab-3,Cell Culture applications,Fluorescence-activated Cell Sorting (FACS),Lab notebook evaluation,Immunohistochemistry,Medicinal Chemistry and drup applicaitons,Genomics and Proteomics Methodology,Microscopy Imaging and analysis,Medical Research Centre (MEDITAM) and experimental animal models-I,Medical Research Centre (MEDITAM) and experimental animal models-II,Final.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Explain the advanced experimental methods and analysis approaches in molecular miology, proteomics, genomics, immunohistochemistry, imaging systems and analysis, cell culture and experimental animal laboratories.
14, 17, 2, 9
A, E
Incorporate, do research and apply new research techniques with the thesis subject.
14, 17, 2, 9
A, E
Explain and provide solutions to various different experimental techniques.
14, 17, 2, 9
A, E
Define the principles of basic research laboratory equipments and can operate these devices.
14, 17, 2, 9
A, E
Explains the advanced data analysis approaches of scientific data.
14, 17, 2, 9
A, E
Explain and evaluate the workplace and biological safety instructions in the laboratory.
14, 17, 2, 9
A, E
Teaching Methods:
14: Self Study Method, 17: Experimental Technique, 2: Project Based Learning Model, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, E: Homework
Course Outline
Order
Subjects
Preliminary Work
1
Advanced Molecular Biology Techniques-1, Lab-1
Self-study
2
Advanced Molecular Biology Techniques-2, Lab-2
Self-study
3
Electrophysiology laboratory
Self-study
4
Advanced Molecular Biology Techniques-3, Lab-3
Self-study
5
Cell Culture applications
Self-study
6
Fluorescence-activated Cell Sorting (FACS)
Self-study
7
Lab notebook evaluation
Self-study and lecture notes
8
Immunohistochemistry
Self-study
9
Medicinal Chemistry and drup applicaitons
Self-study
10
Genomics and Proteomics Methodology
Self-study
11
Microscopy Imaging and analysis
Self-study
12
Medical Research Centre (MEDITAM) and experimental animal models-I
Self-study
13
Medical Research Centre (MEDITAM) and experimental animal models-II
Self-study
14
Final
Self-study and lecture notes
Resources
Laboratory Biosafety Manuel(Third edition) 1. Containment of biohazard 2. Laboratoaries - standards 3. Laboratory infection - prevention and control 4. Manuals I. Title . ISBN 92 4 154650 6 (LC/NLM classification: QY 25) WHO/ CDS/ CSR/ LYO2004. 11
Internet database
Course Contribution to Program Qualifications
Course Contribution to Program Qualifications
No
Program Qualification
Contribution Level
1
2
3
4
5
1
Defines macro and micromolecules in cell and body fluids at an advanced level.
X
2
Knows how to store and protect the material to be analyzed.
X
3
Knows how to identify molecules found in cells and body fluids.
X
4
Knows the principles of technologies to be used for routine and research purposes.
X
5
Knows how to evaluate the results of the study, interprets and questions.
6
Knows how to use field-specific tools and equipment
X
7
Knows how to access and interpret field-specific resources and information
8
Shares information with disciplines related to the field
X
9
Combines theoretical and factual knowledge with critical thoughts, conducts independent studies, evaluates the results, compares them with the literature, and gets them accepted by authors and scientific journals.
10
Solves a scientific and technical problem, knows where to get help.
X
11
Takes responsibility in joint scientific studies, contributes and works in harmony.
12
Participates in meetings related to the field of biochemistry, presents papers, participates in discussions and congresses, publishes studies in reputable journals specific to the field.
13
Collects, interprets, applies, announces data related to the field of biochemistry, conducts social and cultural studies, and supervises these studies scientifically and socially.
X
14
Can take part in management activities, evaluate the results obtained to improve quality
Assessment Methods
Contribution Level
Absolute Evaluation
Rate of Midterm Exam to Success
50
Rate of Final Exam to Success
50
Total
100
ECTS / Workload Table
Activities
Number of
Duration(Hour)
Total Workload(Hour)
Course Hours
14
3
42
Guided Problem Solving
14
10
140
Resolution of Homework Problems and Submission as a Report
0
0
0
Term Project
0
0
0
Presentation of Project / Seminar
0
0
0
Quiz
0
0
0
Midterm Exam
0
0
0
General Exam
0
0
0
Performance Task, Maintenance Plan
0
0
0
Total Workload(Hour)
182
Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(182/30)
6
ECTS of the course: 30 hours of work is counted as 1 ECTS credit.
Course Supervisor: Prof. Dr Esra Çağavi,
Course LaboAssistants: Laboratory supervisor from Research Institute for Health Sciences and Technologies (SABITA),
Aim
The aim of the “Laboratory Rotation-I” course is for Ph.D. level students to explain the advanced technical approaches in scientific research, the basic laboratory equipment usage instructions, and laboratory application that will be incorporated to the thesis work by understanding the scope of different laboratory disciplines. By rotating in different labs every week, students are aimed to explain laboratory safety and instructions and practice advanced molecular biology techniques, proteomics and genomics applications, flow cytometry and analysis, histology applications, microscopy imaging systems and image analysis, cell culture and experimental animal laboratories.
Course Content
This course contains; Advanced Molecular Biology Techniques-1, Lab-1,Advanced Molecular Biology Techniques-2, Lab-2,Electrophysiology laboratory,Advanced Molecular Biology Techniques-3, Lab-3,Cell Culture applications,Fluorescence-activated Cell Sorting (FACS),Lab notebook evaluation,Immunohistochemistry,Medicinal Chemistry and drup applicaitons,Genomics and Proteomics Methodology,Microscopy Imaging and analysis,Medical Research Centre (MEDITAM) and experimental animal models-I,Medical Research Centre (MEDITAM) and experimental animal models-II,Final.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Explain the advanced experimental methods and analysis approaches in molecular miology, proteomics, genomics, immunohistochemistry, imaging systems and analysis, cell culture and experimental animal laboratories.
14, 17, 2, 9
A, E
Incorporate, do research and apply new research techniques with the thesis subject.
14, 17, 2, 9
A, E
Explain and provide solutions to various different experimental techniques.
14, 17, 2, 9
A, E
Define the principles of basic research laboratory equipments and can operate these devices.
14, 17, 2, 9
A, E
Explains the advanced data analysis approaches of scientific data.
14, 17, 2, 9
A, E
Explain and evaluate the workplace and biological safety instructions in the laboratory.
14, 17, 2, 9
A, E
Teaching Methods:
14: Self Study Method, 17: Experimental Technique, 2: Project Based Learning Model, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, E: Homework
Course Outline
Order
Subjects
Preliminary Work
1
Advanced Molecular Biology Techniques-1, Lab-1
Self-study
2
Advanced Molecular Biology Techniques-2, Lab-2
Self-study
3
Electrophysiology laboratory
Self-study
4
Advanced Molecular Biology Techniques-3, Lab-3
Self-study
5
Cell Culture applications
Self-study
6
Fluorescence-activated Cell Sorting (FACS)
Self-study
7
Lab notebook evaluation
Self-study and lecture notes
8
Immunohistochemistry
Self-study
9
Medicinal Chemistry and drup applicaitons
Self-study
10
Genomics and Proteomics Methodology
Self-study
11
Microscopy Imaging and analysis
Self-study
12
Medical Research Centre (MEDITAM) and experimental animal models-I
Self-study
13
Medical Research Centre (MEDITAM) and experimental animal models-II
Self-study
14
Final
Self-study and lecture notes
Resources
Laboratory Biosafety Manuel(Third edition) 1. Containment of biohazard 2. Laboratoaries - standards 3. Laboratory infection - prevention and control 4. Manuals I. Title . ISBN 92 4 154650 6 (LC/NLM classification: QY 25) WHO/ CDS/ CSR/ LYO2004. 11
Internet database
Course Contribution to Program Qualifications
Course Contribution to Program Qualifications
No
Program Qualification
Contribution Level
1
2
3
4
5
1
Defines macro and micromolecules in cell and body fluids at an advanced level.
X
2
Knows how to store and protect the material to be analyzed.
X
3
Knows how to identify molecules found in cells and body fluids.
X
4
Knows the principles of technologies to be used for routine and research purposes.
X
5
Knows how to evaluate the results of the study, interprets and questions.
6
Knows how to use field-specific tools and equipment
X
7
Knows how to access and interpret field-specific resources and information
8
Shares information with disciplines related to the field
X
9
Combines theoretical and factual knowledge with critical thoughts, conducts independent studies, evaluates the results, compares them with the literature, and gets them accepted by authors and scientific journals.
10
Solves a scientific and technical problem, knows where to get help.
X
11
Takes responsibility in joint scientific studies, contributes and works in harmony.
12
Participates in meetings related to the field of biochemistry, presents papers, participates in discussions and congresses, publishes studies in reputable journals specific to the field.
13
Collects, interprets, applies, announces data related to the field of biochemistry, conducts social and cultural studies, and supervises these studies scientifically and socially.
X
14
Can take part in management activities, evaluate the results obtained to improve quality
