Course Supervisor: Prof. Dr Esra Çağavi, Course LaboAssistants: Laboratory supervisor from Research Institute for Health Sciences and Technologies (SABITA),
Aim
The aim of the “Laboratory Rotation-II” course is for Ph.D. level students to to explain the advanced technical approaches in laboratory application that is expected to incorporate in their thesis work understanding of the scope of different laboratory disciplines. By rotating in different labs every week, students are aimed to be exposed to the advanced molecular biology techniques, proteomics and genomics applications, FACS, primary and cell line culture applications, transgenic animal models, electrophysiology, neuroscience and cancer research applications, basic protein and gene expression analysis.
Course Content
This course contains; Electroencephalography (EEG) recording principles
,EEG application and signal analysis,Flourescence activated cell sorting (FACS) method and analysis ,Functional Neurology Applications,Flourescence activated cell sorting (FACS) method and analysis ,Experimental and transgenic animal models and applications, Evaluation of Laboratory Notebooks for the Midterm Exam,Cell survival tests analysis,Electrophysiology applications and analysis,Quantitative RTPCR analysis in Molecular Biology,Cancer Biology Lab and Applications,Immunohistochemistry and cryosectioning ,SDS-PAGE and Western blot analysis,Evaluation of Laboratory Notebooks for the Final Exam.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Explain the advanced culture methods, advanced approaches in molecular biology, experimental transgenic animal models and their applications, applications in neuroscience research, EEG principles and electrophsiology, fundamental approaches in cancer biology, cell survival analysis and FACS analysis.
14, 17, 3, 9
A, E
Explains advanced research techniques.
14, 17, 3, 9
A, E
Explain and provide solutions to various different experimental techniques.
14, 17, 3, 9
A, E
Define the principles of basic research laboratory equipments and can operate these devices.
14, 17, 3, 9
A, E
Explains the advanced data analysis approaches of scientific data.
14, 17, 3, 9
A, E
Teaching Methods:
14: Self Study Method, 17: Experimental Technique, 3: Problem Baded Learning Model, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, E: Homework
Course Outline
Order
Subjects
Preliminary Work
1
Electroencephalography (EEG) recording principles
Self-study
2
EEG application and signal analysis
3
Flourescence activated cell sorting (FACS) method and analysis
Self-study
4
Functional Neurology Applications
Self-study
5
Flourescence activated cell sorting (FACS) method and analysis
Self-study
6
Experimental and transgenic animal models and applications
Self-study
7
Evaluation of Laboratory Notebooks for the Midterm Exam
Self-study
8
Cell survival tests analysis
9
Electrophysiology applications and analysis
10
Quantitative RTPCR analysis in Molecular Biology
11
Cancer Biology Lab and Applications
12
Immunohistochemistry and cryosectioning
13
SDS-PAGE and Western blot analysis
14
Evaluation of Laboratory Notebooks for the Final Exam
Defines macro and micromolecules in cell and body fluids at an advanced level.
X
2
Knows how to store and protect the material to be analyzed.
X
3
Knows how to identify molecules found in cells and body fluids.
X
4
Knows the principles of technologies to be used for routine and research purposes.
X
5
Knows how to evaluate the results of the study, interprets and questions.
X
6
Knows how to use field-specific tools and equipment
X
7
Knows how to access and interpret field-specific resources and information
8
Shares information with disciplines related to the field
X
9
Combines theoretical and factual knowledge with critical thoughts, conducts independent studies, evaluates the results, compares them with the literature, and gets them accepted by authors and scientific journals.
10
Solves a scientific and technical problem, knows where to get help.
X
11
Takes responsibility in joint scientific studies, contributes and works in harmony.
12
Participates in meetings related to the field of biochemistry, presents papers, participates in discussions and congresses, publishes studies in reputable journals specific to the field.
X
13
Collects, interprets, applies, announces data related to the field of biochemistry, conducts social and cultural studies, and supervises these studies scientifically and socially.
14
Can take part in management activities, evaluate the results obtained to improve quality
Assessment Methods
Contribution Level
Absolute Evaluation
Rate of Midterm Exam to Success
50
Rate of Final Exam to Success
50
Total
100
ECTS / Workload Table
Activities
Number of
Duration(Hour)
Total Workload(Hour)
Course Hours
14
3
42
Guided Problem Solving
5
6
30
Resolution of Homework Problems and Submission as a Report
14
6
84
Term Project
0
0
0
Presentation of Project / Seminar
0
0
0
Quiz
0
0
0
Midterm Exam
1
10
10
General Exam
1
10
10
Performance Task, Maintenance Plan
0
0
0
Total Workload(Hour)
176
Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(176/30)
6
ECTS of the course: 30 hours of work is counted as 1 ECTS credit.
Course Supervisor: Prof. Dr Esra Çağavi, Course LaboAssistants: Laboratory supervisor from Research Institute for Health Sciences and Technologies (SABITA),
Aim
The aim of the “Laboratory Rotation-II” course is for Ph.D. level students to to explain the advanced technical approaches in laboratory application that is expected to incorporate in their thesis work understanding of the scope of different laboratory disciplines. By rotating in different labs every week, students are aimed to be exposed to the advanced molecular biology techniques, proteomics and genomics applications, FACS, primary and cell line culture applications, transgenic animal models, electrophysiology, neuroscience and cancer research applications, basic protein and gene expression analysis.
Course Content
This course contains; Electroencephalography (EEG) recording principles
,EEG application and signal analysis,Flourescence activated cell sorting (FACS) method and analysis ,Functional Neurology Applications,Flourescence activated cell sorting (FACS) method and analysis ,Experimental and transgenic animal models and applications, Evaluation of Laboratory Notebooks for the Midterm Exam,Cell survival tests analysis,Electrophysiology applications and analysis,Quantitative RTPCR analysis in Molecular Biology,Cancer Biology Lab and Applications,Immunohistochemistry and cryosectioning ,SDS-PAGE and Western blot analysis,Evaluation of Laboratory Notebooks for the Final Exam.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Explain the advanced culture methods, advanced approaches in molecular biology, experimental transgenic animal models and their applications, applications in neuroscience research, EEG principles and electrophsiology, fundamental approaches in cancer biology, cell survival analysis and FACS analysis.
14, 17, 3, 9
A, E
Explains advanced research techniques.
14, 17, 3, 9
A, E
Explain and provide solutions to various different experimental techniques.
14, 17, 3, 9
A, E
Define the principles of basic research laboratory equipments and can operate these devices.
14, 17, 3, 9
A, E
Explains the advanced data analysis approaches of scientific data.
14, 17, 3, 9
A, E
Teaching Methods:
14: Self Study Method, 17: Experimental Technique, 3: Problem Baded Learning Model, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, E: Homework
Course Outline
Order
Subjects
Preliminary Work
1
Electroencephalography (EEG) recording principles
Self-study
2
EEG application and signal analysis
3
Flourescence activated cell sorting (FACS) method and analysis
Self-study
4
Functional Neurology Applications
Self-study
5
Flourescence activated cell sorting (FACS) method and analysis
Self-study
6
Experimental and transgenic animal models and applications
Self-study
7
Evaluation of Laboratory Notebooks for the Midterm Exam
Self-study
8
Cell survival tests analysis
9
Electrophysiology applications and analysis
10
Quantitative RTPCR analysis in Molecular Biology
11
Cancer Biology Lab and Applications
12
Immunohistochemistry and cryosectioning
13
SDS-PAGE and Western blot analysis
14
Evaluation of Laboratory Notebooks for the Final Exam
Defines macro and micromolecules in cell and body fluids at an advanced level.
X
2
Knows how to store and protect the material to be analyzed.
X
3
Knows how to identify molecules found in cells and body fluids.
X
4
Knows the principles of technologies to be used for routine and research purposes.
X
5
Knows how to evaluate the results of the study, interprets and questions.
X
6
Knows how to use field-specific tools and equipment
X
7
Knows how to access and interpret field-specific resources and information
8
Shares information with disciplines related to the field
X
9
Combines theoretical and factual knowledge with critical thoughts, conducts independent studies, evaluates the results, compares them with the literature, and gets them accepted by authors and scientific journals.
10
Solves a scientific and technical problem, knows where to get help.
X
11
Takes responsibility in joint scientific studies, contributes and works in harmony.
12
Participates in meetings related to the field of biochemistry, presents papers, participates in discussions and congresses, publishes studies in reputable journals specific to the field.
X
13
Collects, interprets, applies, announces data related to the field of biochemistry, conducts social and cultural studies, and supervises these studies scientifically and socially.
14
Can take part in management activities, evaluate the results obtained to improve quality