Prof.Dr. Esra ÇAĞAVİ, Prof.Dr. Kürşat ÖZDİLLİ, Assist.Prof. Neşe AYŞİT, Assoc.Prof. Nihal KARAKAŞ, Assist.Prof. Berrak ÇAĞLAYAN, Assist.Prof. İlyas ÖZÇİÇEK, Assist.Prof. Salih GENCER, Assist.Prof. Elif Zeynep YILMAZ, Assist.Prof. Sven Pierre L. VILAIN
Assistant(s)
Prof.Dr. Esra ÇAĞAVİ, Prof.Dr. Kürşat ÖZDİLLİ, Dr.Öğr.Üye. Neşe AYŞİT, Doç.Dr. Nihal KARAKAŞ, Dr.Öğr.Üye. Berrak ÇAĞLAYAN, Dr.Öğr.Üye. İlyas ÖZÇİÇEK, Dr.Öğr.Üye. Salih GENCER, Dr.Öğr.Üye. Elif Zeynep YILMAZ, Dr.Öğr.Üye. Sven Pierre L. VILAIN, Dr.Öğr.Üye. Muzaffer ARIKAN
Aim
This course aims to enable students to understand and practice basic molecular techniques at the RNA and protein level, cell culture and imaging, and basic tissue treatment techniques.
Course Content
This course contains; Preparation of solutions, PH adjustment and autoclave use,Polimerase Chain Reaction (PCR) and agarose gel electrophoresis,Quantitative real time PCR reaksiyonu ve analizi,Preparation and running of SDS-PAGE,Western Blot analysis
,Cell Survial Assays (MTT etc),Basic Statistical Analysis,Cell Culture rules and principles, Cell Culture passaging, counting and thaw,Basics of Immunocytochemistry,Tissue Preservation methodologies and tissue sectioning,Basics of immunohistochemistry,Immunoflourescence Microscopy Applications,Image Analysis applications.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Explains basic molecular techniques at the RNA and protein level
13, 14, 17, 9
A, E
Explains the working principles of techniques such as Western Blot, qRTPCR etc.
13, 14, 17, 9
A, E
Explains cell culture principles
13, 14, 17, 9
A, E
Interprets the immunostaining and fluorescent imaging methods at cells and tissues.
13, 14, 17, 9
A, E
Teaching Methods:
13: Case Study Method, 14: Self Study Method, 17: Experimental Technique, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, E: Homework
Course Outline
Order
Subjects
Preliminary Work
1
Preparation of solutions, PH adjustment and autoclave use
2
Polimerase Chain Reaction (PCR) and agarose gel electrophoresis
3
Quantitative real time PCR reaksiyonu ve analizi
4
Preparation and running of SDS-PAGE
5
Western Blot analysis
6
Cell Survial Assays (MTT etc)
7
Basic Statistical Analysis
8
Cell Culture rules and principles
9
Cell Culture passaging, counting and thaw
10
Basics of Immunocytochemistry
11
Tissue Preservation methodologies and tissue sectioning
12
Basics of immunohistochemistry
13
Immunoflourescence Microscopy Applications
14
Image Analysis applications
Resources
The technical information and manuals provided in the labs
Course Contribution to Program Qualifications
Course Contribution to Program Qualifications
No
Program Qualification
Contribution Level
1
2
3
4
5
1
- Defines cell structure, functions and different cell types.
X
2
Defines the organelles of the cell, their functions and their communication with each other.
X
3
Explains the basic structure and function of the cell.
X
4
Explains laboratory equipment and their use.
X
5
Defines the use of basic laboratory devices.
X
6
Explains the rules and safety issues to be followed in the laboratory.
X
7
Can develop a hypothesis for a scientific study based ona scientific question.
X
8
Can participate in a clinical or non-clinical study and suggest methodological strategy.
X
9
Can explore advanced biological application and develop laboratory skills to implements and teach them effectively when necessary.
X
10
Can plan scientific research.
X
11
Can use scientific databases.
X
12
Carries out a given task independently by using the basic knowledge he/she has in his/her field.
X
13
Evaluates the basic level of knowledge and skills acquired in the field with a critical approach; identifies learning needs and directs learning.
X
14
He/she can exchange information and ideas by communicating with others about his/her field and scientific research issues.
X
15
Follows the literature with advanced foreign language and computer skills.
X
16
Acts in accordance with the laws, regulations, legislation and professional ethical rules regarding his duties, rights and responsibilities as a Medical Biology Master degree holder.
X
17
Carries out a given task independently by using the basic knowledge he/she has in his/her field.
X
Assessment Methods
Contribution Level
Absolute Evaluation
Rate of Midterm Exam to Success
50
Rate of Final Exam to Success
50
Total
100
ECTS / Workload Table
Activities
Number of
Duration(Hour)
Total Workload(Hour)
Course Hours
14
3
42
Guided Problem Solving
14
2
28
Resolution of Homework Problems and Submission as a Report
14
3
42
Term Project
0
0
0
Presentation of Project / Seminar
0
0
0
Quiz
0
0
0
Midterm Exam
1
30
30
General Exam
1
30
30
Performance Task, Maintenance Plan
0
0
0
Total Workload(Hour)
172
Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(172/30)
6
ECTS of the course: 30 hours of work is counted as 1 ECTS credit.
Detail Informations of the Course
Course Description
Course
Code
Semester
T+P (Hour)
Credit
ECTS
ADVANCED MOLECULAR TECHNIQUES
-
Spring Semester
1+2
2
6
Course Program
Prerequisites Courses
Recommended Elective Courses
Language of Course
Turkish
Course Level
Second Cycle (Master's Degree)
Course Type
Elective
Course Coordinator
Prof.Dr. Esra ÇAĞAVİ
Name of Lecturer(s)
Prof.Dr. Esra ÇAĞAVİ, Prof.Dr. Kürşat ÖZDİLLİ, Assist.Prof. Neşe AYŞİT, Assoc.Prof. Nihal KARAKAŞ, Assist.Prof. Berrak ÇAĞLAYAN, Assist.Prof. İlyas ÖZÇİÇEK, Assist.Prof. Salih GENCER, Assist.Prof. Elif Zeynep YILMAZ, Assist.Prof. Sven Pierre L. VILAIN
Assistant(s)
Prof.Dr. Esra ÇAĞAVİ, Prof.Dr. Kürşat ÖZDİLLİ, Dr.Öğr.Üye. Neşe AYŞİT, Doç.Dr. Nihal KARAKAŞ, Dr.Öğr.Üye. Berrak ÇAĞLAYAN, Dr.Öğr.Üye. İlyas ÖZÇİÇEK, Dr.Öğr.Üye. Salih GENCER, Dr.Öğr.Üye. Elif Zeynep YILMAZ, Dr.Öğr.Üye. Sven Pierre L. VILAIN, Dr.Öğr.Üye. Muzaffer ARIKAN
Aim
This course aims to enable students to understand and practice basic molecular techniques at the RNA and protein level, cell culture and imaging, and basic tissue treatment techniques.
Course Content
This course contains; Preparation of solutions, PH adjustment and autoclave use,Polimerase Chain Reaction (PCR) and agarose gel electrophoresis,Quantitative real time PCR reaksiyonu ve analizi,Preparation and running of SDS-PAGE,Western Blot analysis
,Cell Survial Assays (MTT etc),Basic Statistical Analysis,Cell Culture rules and principles, Cell Culture passaging, counting and thaw,Basics of Immunocytochemistry,Tissue Preservation methodologies and tissue sectioning,Basics of immunohistochemistry,Immunoflourescence Microscopy Applications,Image Analysis applications.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Explains basic molecular techniques at the RNA and protein level
13, 14, 17, 9
A, E
Explains the working principles of techniques such as Western Blot, qRTPCR etc.
13, 14, 17, 9
A, E
Explains cell culture principles
13, 14, 17, 9
A, E
Interprets the immunostaining and fluorescent imaging methods at cells and tissues.
13, 14, 17, 9
A, E
Teaching Methods:
13: Case Study Method, 14: Self Study Method, 17: Experimental Technique, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, E: Homework
Course Outline
Order
Subjects
Preliminary Work
1
Preparation of solutions, PH adjustment and autoclave use
2
Polimerase Chain Reaction (PCR) and agarose gel electrophoresis
3
Quantitative real time PCR reaksiyonu ve analizi
4
Preparation and running of SDS-PAGE
5
Western Blot analysis
6
Cell Survial Assays (MTT etc)
7
Basic Statistical Analysis
8
Cell Culture rules and principles
9
Cell Culture passaging, counting and thaw
10
Basics of Immunocytochemistry
11
Tissue Preservation methodologies and tissue sectioning
12
Basics of immunohistochemistry
13
Immunoflourescence Microscopy Applications
14
Image Analysis applications
Resources
The technical information and manuals provided in the labs
Course Contribution to Program Qualifications
Course Contribution to Program Qualifications
No
Program Qualification
Contribution Level
1
2
3
4
5
1
- Defines cell structure, functions and different cell types.
X
2
Defines the organelles of the cell, their functions and their communication with each other.
X
3
Explains the basic structure and function of the cell.
X
4
Explains laboratory equipment and their use.
X
5
Defines the use of basic laboratory devices.
X
6
Explains the rules and safety issues to be followed in the laboratory.
X
7
Can develop a hypothesis for a scientific study based ona scientific question.
X
8
Can participate in a clinical or non-clinical study and suggest methodological strategy.
X
9
Can explore advanced biological application and develop laboratory skills to implements and teach them effectively when necessary.
X
10
Can plan scientific research.
X
11
Can use scientific databases.
X
12
Carries out a given task independently by using the basic knowledge he/she has in his/her field.
X
13
Evaluates the basic level of knowledge and skills acquired in the field with a critical approach; identifies learning needs and directs learning.
X
14
He/she can exchange information and ideas by communicating with others about his/her field and scientific research issues.
X
15
Follows the literature with advanced foreign language and computer skills.
X
16
Acts in accordance with the laws, regulations, legislation and professional ethical rules regarding his duties, rights and responsibilities as a Medical Biology Master degree holder.
X
17
Carries out a given task independently by using the basic knowledge he/she has in his/her field.
