The aim of this course is to learn basic principles of cell culture and laboratory essentials, and to experience cell culture techniques.
Course Content
This course contains; Introduction to Cell Culture ,Cell Culture Lab-Stucture, Devices and Equipments,Hücre Kültürü Lab-Sarflar,Hücre Kültürü Lab-Aseptik koşullar ve Kontaminasyon Riskleri,Hücre Kültürü-Pratik I: Hücrelerin Alt Kültürlerinin Yapılması,Cell Culture Lab-Practice II-Cell Counting,Cell Culture Lab-Practice III: Cell Freezing,Cell Culture Lab-Practice IV: Cell Thawing,Cell Culture Lab-Practice V: Cell Imaging,Cell Culture Applications,3D Cell Cultures ve Organoidler,Cell Based Therapeutics,Research Article Presentation.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Defines the differences of primary and secondary cells (cell lines); explains how to obtain these cells; defines the consumables, their usage conditions and necessities in a cell culture lab, explains structure of a cell culture lab and tells the essential equipments and devices; experiences subculturing, counting, freezing-thawing and imaging of cells; performs the assigned task in a group work.
10, 11, 12, 13, 16, 17, 2, 3, 30, 37, 4, 6, 9
A, C, G, H, K
Teaching Methods:
10: Discussion Method, 11: Demonstration Method, 12: Problem Solving Method, 13: Case Study Method, 16: Question - Answer Technique, 17: Experimental Technique, 2: Project Based Learning Model, 3: Problem Baded Learning Model, 30: Predict-Observe-Explain Techniques, 37: Computer-Internet Supported Instruction, 4: Inquiry-Based Learning, 6: Experiential Learning, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, C: Multiple-Choice Exam, G: Quiz, H: Performance Task, K: Self Assessment Technique
Course Outline
Order
Subjects
Preliminary Work
1
Introduction to Cell Culture
Reading
2
Cell Culture Lab-Stucture, Devices and Equipments
Onsite laboratory introduction
3
Hücre Kültürü Lab-Sarflar
4
Hücre Kültürü Lab-Aseptik koşullar ve Kontaminasyon Riskleri
5
Hücre Kültürü-Pratik I: Hücrelerin Alt Kültürlerinin Yapılması
Preperation of essential consumables and tools
6
Cell Culture Lab-Practice II-Cell Counting
Preperation of essential consumables and tools
8
Cell Culture Lab-Practice III: Cell Freezing
Preperation of required consumables and tools
9
Cell Culture Lab-Practice IV: Cell Thawing
Preperation of essential consumables and tools
10
Cell Culture Lab-Practice V: Cell Imaging
Preperation of required consumables and tools
11
Cell Culture Applications
Sunum hazırlama
12
3D Cell Cultures ve Organoidler
Reading
13
Cell Based Therapeutics
Reading
14
Research Article Presentation
Sunum hazırlama
Resources
Laboratory manual and lecture notes
Course Contribution to Program Qualifications
Course Contribution to Program Qualifications
No
Program Qualification
Contribution Level
1
2
3
4
5
1
- Defines cell structure, functions and different cell types.
2
Defines the organelles of the cell, their functions and their communication with each other.
3
Explains the basic structure and function of the cell.
4
Explains laboratory equipment and their use.
5
Defines the use of basic laboratory devices.
6
Explains the rules and safety issues to be followed in the laboratory.
7
Can develop a hypothesis for a scientific study based ona scientific question.
8
Can participate in a clinical or non-clinical study and suggest methodological strategy.
9
Can explore advanced biological application and develop laboratory skills to implements and teach them effectively when necessary.
10
Can plan scientific research.
11
Can use scientific databases.
12
Carries out a given task independently by using the basic knowledge he/she has in his/her field.
13
Evaluates the basic level of knowledge and skills acquired in the field with a critical approach; identifies learning needs and directs learning.
14
He/she can exchange information and ideas by communicating with others about his/her field and scientific research issues.
15
Follows the literature with advanced foreign language and computer skills.
16
Acts in accordance with the laws, regulations, legislation and professional ethical rules regarding his duties, rights and responsibilities as a Medical Biology Master degree holder.
17
Carries out a given task independently by using the basic knowledge he/she has in his/her field.
Assessment Methods
Contribution Level
Absolute Evaluation
Rate of Midterm Exam to Success
50
Rate of Final Exam to Success
50
Total
100
ECTS / Workload Table
Activities
Number of
Duration(Hour)
Total Workload(Hour)
Course Hours
14
1
14
Guided Problem Solving
14
2
28
Resolution of Homework Problems and Submission as a Report
0
0
0
Term Project
0
0
0
Presentation of Project / Seminar
0
0
0
Quiz
0
0
0
Midterm Exam
0
0
0
General Exam
0
0
0
Performance Task, Maintenance Plan
0
0
0
Total Workload(Hour)
42
Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(42/30)
1
ECTS of the course: 30 hours of work is counted as 1 ECTS credit.
The aim of this course is to learn basic principles of cell culture and laboratory essentials, and to experience cell culture techniques.
Course Content
This course contains; Introduction to Cell Culture ,Cell Culture Lab-Stucture, Devices and Equipments,Hücre Kültürü Lab-Sarflar,Hücre Kültürü Lab-Aseptik koşullar ve Kontaminasyon Riskleri,Hücre Kültürü-Pratik I: Hücrelerin Alt Kültürlerinin Yapılması,Cell Culture Lab-Practice II-Cell Counting,Cell Culture Lab-Practice III: Cell Freezing,Cell Culture Lab-Practice IV: Cell Thawing,Cell Culture Lab-Practice V: Cell Imaging,Cell Culture Applications,3D Cell Cultures ve Organoidler,Cell Based Therapeutics,Research Article Presentation.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Defines the differences of primary and secondary cells (cell lines); explains how to obtain these cells; defines the consumables, their usage conditions and necessities in a cell culture lab, explains structure of a cell culture lab and tells the essential equipments and devices; experiences subculturing, counting, freezing-thawing and imaging of cells; performs the assigned task in a group work.
10, 11, 12, 13, 16, 17, 2, 3, 30, 37, 4, 6, 9
A, C, G, H, K
Teaching Methods:
10: Discussion Method, 11: Demonstration Method, 12: Problem Solving Method, 13: Case Study Method, 16: Question - Answer Technique, 17: Experimental Technique, 2: Project Based Learning Model, 3: Problem Baded Learning Model, 30: Predict-Observe-Explain Techniques, 37: Computer-Internet Supported Instruction, 4: Inquiry-Based Learning, 6: Experiential Learning, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, C: Multiple-Choice Exam, G: Quiz, H: Performance Task, K: Self Assessment Technique
Course Outline
Order
Subjects
Preliminary Work
1
Introduction to Cell Culture
Reading
2
Cell Culture Lab-Stucture, Devices and Equipments
Onsite laboratory introduction
3
Hücre Kültürü Lab-Sarflar
4
Hücre Kültürü Lab-Aseptik koşullar ve Kontaminasyon Riskleri
5
Hücre Kültürü-Pratik I: Hücrelerin Alt Kültürlerinin Yapılması
Preperation of essential consumables and tools
6
Cell Culture Lab-Practice II-Cell Counting
Preperation of essential consumables and tools
8
Cell Culture Lab-Practice III: Cell Freezing
Preperation of required consumables and tools
9
Cell Culture Lab-Practice IV: Cell Thawing
Preperation of essential consumables and tools
10
Cell Culture Lab-Practice V: Cell Imaging
Preperation of required consumables and tools
11
Cell Culture Applications
Sunum hazırlama
12
3D Cell Cultures ve Organoidler
Reading
13
Cell Based Therapeutics
Reading
14
Research Article Presentation
Sunum hazırlama
Resources
Laboratory manual and lecture notes
Course Contribution to Program Qualifications
Course Contribution to Program Qualifications
No
Program Qualification
Contribution Level
1
2
3
4
5
1
- Defines cell structure, functions and different cell types.
2
Defines the organelles of the cell, their functions and their communication with each other.
3
Explains the basic structure and function of the cell.
4
Explains laboratory equipment and their use.
5
Defines the use of basic laboratory devices.
6
Explains the rules and safety issues to be followed in the laboratory.
7
Can develop a hypothesis for a scientific study based ona scientific question.
8
Can participate in a clinical or non-clinical study and suggest methodological strategy.
9
Can explore advanced biological application and develop laboratory skills to implements and teach them effectively when necessary.
10
Can plan scientific research.
11
Can use scientific databases.
12
Carries out a given task independently by using the basic knowledge he/she has in his/her field.
13
Evaluates the basic level of knowledge and skills acquired in the field with a critical approach; identifies learning needs and directs learning.
14
He/she can exchange information and ideas by communicating with others about his/her field and scientific research issues.
15
Follows the literature with advanced foreign language and computer skills.
16
Acts in accordance with the laws, regulations, legislation and professional ethical rules regarding his duties, rights and responsibilities as a Medical Biology Master degree holder.
17
Carries out a given task independently by using the basic knowledge he/she has in his/her field.
