The aim of this course is to teach the structure of the cell, examination of cytoplasmic organelles and cell nucleus, cell division, types of microscopes and their usage characteristics, main techniques used in histological studies, immunohistochemical techniques, electron microscope and its properties, preparation of hard and soft tissues for TEM, tissue samples taking, fixation, washing, dehydration methods, placement of samples, blocking and examination in TEM, to the course taking student.
Course Content
This course contains; Introduction to immunohistochemistry,Antigen, antibody structure,Obtaining monoclonal and polyclonal primary antibodies,Immunohistochemistry stages,Antigen retrieval techniques,Chromogens,Midterm exam,Immunofluorescence method,Practice,Problems and solutions that may be encountered in immunohistochemistry application,Imaging and analyzing under a light microscope,Practice,Practice,Final exam.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Analyzes the prepared preparations.
12, 14, 9
A
Define the difference between monoclonal and polyclonal antibodies.
14, 9
A, E
Determines the problems that may be encountered during the application of the immunohistochemistry method.
14, 9
A, E
Explains basic information about antigen retrieval techniques.
14, 9
A
Explains basic information about antigen and antibody structure.
14, 9
A, E
It solves the problems that may be encountered during the application of the immunohistochemistry method.
12, 14, 9
A
Explains the stages of the immunofluorescence method.
14, 9
A
Explains immunohistochemical methods.
14, 9
A
Analyzes images obtained with a light microscope.
14, 9
A, E
It solves the problems that may be encountered during the application of the immunofluorescence method.
12, 14, 9
A
Teaching Methods:
12: Problem Solving Method, 14: Self Study Method, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, E: Homework
Course Outline
Order
Subjects
Preliminary Work
1
Introduction to immunohistochemistry
Reading the relevant course presentation
2
Antigen, antibody structure
Reading the relevant course presentation
3
Obtaining monoclonal and polyclonal primary antibodies
Reading the relevant course presentation
4
Immunohistochemistry stages
Reading the relevant course presentation
5
Antigen retrieval techniques
Reading the relevant course presentation
6
Chromogens
Histological Staining Techniques, Prof. Dr. Ramazan Demir, Palme Publishing. Page: 301-303
7
Midterm exam
-
8
Immunofluorescence method
Histological Staining Techniques, Prof. Dr. Ramazan Demir, Palme Publishing. Page: 296-298.
9
Practice
Histological Staining Techniques, Prof. Dr. Ramazan Demir, Palme Publishing. Page: 296-298.
10
Problems and solutions that may be encountered in immunohistochemistry application
Reading the relevant course presentation
11
Imaging and analyzing under a light microscope
-
12
Practice
Histological Staining Techniques, Prof. Dr. Ramazan Demir, Palme Publishing. Page: 289-291.
13
Practice
Histological Staining Techniques, Prof. Dr. Ramazan Demir, Palme Publishing. Page: 291-292.
14
Final exam
-
Resources
Lecture notes,
Bancroft's Theory and Practice of Histological Techniques (Editors:Kim S Suvarna, Christopher Layton, John D. Bancroft),
Histological staining techniques (Prof. Dr. Ramazan Demir),
Electron Microscopy Methods and Protocols (Editors: Kuo, John)
Course Contribution to Program Qualifications
Course Contribution to Program Qualifications
No
Program Qualification
Contribution Level
1
2
3
4
5
1
Know about histology of basic tissue, basic embryological development, mechanisms of routine micro-preparation techniques, and the basics of active use of a microscope
X
2
Have knowledge of ethics of research and the rules of documenting a research
3
Can relay information about basic histology-embryology
4
Can work on tissue preservation, tissue dye and do microscopic examination.
X
5
Can do literature review
X
6
Can attend both clinical and nonclinical studies and make proposals for methods.
X
7
Can document outcomes.
X
8
Express themselves by using resources in Histology-Embryology and in the areas of histotechnical science, and communication techniques.
X
9
Improve their critical thinking, and can create strategies.
X
10
Behave with an ethical concern in both clinical and nonclinical preparation.
11
Have the awareness of business ethics and create a secure working environment, evaluate the contributors.
12
Exhibit scientific assets in national or international scientific meetings using foreign languages besides their mother language.
13
Promote interdisciplinary cooperation with the consciousness of social responsibility and remain true to professional secrecy, create final decleration within the framework of the quality.
Assessment Methods
Contribution Level
Absolute Evaluation
Rate of Midterm Exam to Success
50
Rate of Final Exam to Success
50
Total
100
ECTS / Workload Table
Activities
Number of
Duration(Hour)
Total Workload(Hour)
Course Hours
14
6
84
Guided Problem Solving
8
3
24
Resolution of Homework Problems and Submission as a Report
7
10
70
Term Project
3
10
30
Presentation of Project / Seminar
0
0
0
Quiz
0
0
0
Midterm Exam
1
15
15
General Exam
1
15
15
Performance Task, Maintenance Plan
0
0
0
Total Workload(Hour)
238
Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(238/30)
8
ECTS of the course: 30 hours of work is counted as 1 ECTS credit.
Detail Informations of the Course
Course Description
Course
Code
Semester
T+P (Hour)
Credit
ECTS
HISTOTECHNOLOGY-II
-
Spring Semester
1+4
3
8
Course Program
Prerequisites Courses
Recommended Elective Courses
Language of Course
Turkish
Course Level
Second Cycle (Master's Degree)
Course Type
Required
Course Coordinator
Assist.Prof. Bircan KOLBAŞI
Name of Lecturer(s)
Prof.Dr. İlknur KESKİN
Assistant(s)
Aim
The aim of this course is to teach the structure of the cell, examination of cytoplasmic organelles and cell nucleus, cell division, types of microscopes and their usage characteristics, main techniques used in histological studies, immunohistochemical techniques, electron microscope and its properties, preparation of hard and soft tissues for TEM, tissue samples taking, fixation, washing, dehydration methods, placement of samples, blocking and examination in TEM, to the course taking student.
Course Content
This course contains; Introduction to immunohistochemistry,Antigen, antibody structure,Obtaining monoclonal and polyclonal primary antibodies,Immunohistochemistry stages,Antigen retrieval techniques,Chromogens,Midterm exam,Immunofluorescence method,Practice,Problems and solutions that may be encountered in immunohistochemistry application,Imaging and analyzing under a light microscope,Practice,Practice,Final exam.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Analyzes the prepared preparations.
12, 14, 9
A
Define the difference between monoclonal and polyclonal antibodies.
14, 9
A, E
Determines the problems that may be encountered during the application of the immunohistochemistry method.
14, 9
A, E
Explains basic information about antigen retrieval techniques.
14, 9
A
Explains basic information about antigen and antibody structure.
14, 9
A, E
It solves the problems that may be encountered during the application of the immunohistochemistry method.
12, 14, 9
A
Explains the stages of the immunofluorescence method.
14, 9
A
Explains immunohistochemical methods.
14, 9
A
Analyzes images obtained with a light microscope.
14, 9
A, E
It solves the problems that may be encountered during the application of the immunofluorescence method.
12, 14, 9
A
Teaching Methods:
12: Problem Solving Method, 14: Self Study Method, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, E: Homework
Course Outline
Order
Subjects
Preliminary Work
1
Introduction to immunohistochemistry
Reading the relevant course presentation
2
Antigen, antibody structure
Reading the relevant course presentation
3
Obtaining monoclonal and polyclonal primary antibodies
Reading the relevant course presentation
4
Immunohistochemistry stages
Reading the relevant course presentation
5
Antigen retrieval techniques
Reading the relevant course presentation
6
Chromogens
Histological Staining Techniques, Prof. Dr. Ramazan Demir, Palme Publishing. Page: 301-303
7
Midterm exam
-
8
Immunofluorescence method
Histological Staining Techniques, Prof. Dr. Ramazan Demir, Palme Publishing. Page: 296-298.
9
Practice
Histological Staining Techniques, Prof. Dr. Ramazan Demir, Palme Publishing. Page: 296-298.
10
Problems and solutions that may be encountered in immunohistochemistry application
Reading the relevant course presentation
11
Imaging and analyzing under a light microscope
-
12
Practice
Histological Staining Techniques, Prof. Dr. Ramazan Demir, Palme Publishing. Page: 289-291.
13
Practice
Histological Staining Techniques, Prof. Dr. Ramazan Demir, Palme Publishing. Page: 291-292.
14
Final exam
-
Resources
Lecture notes,
Bancroft's Theory and Practice of Histological Techniques (Editors:Kim S Suvarna, Christopher Layton, John D. Bancroft),
Histological staining techniques (Prof. Dr. Ramazan Demir),
Electron Microscopy Methods and Protocols (Editors: Kuo, John)
Course Contribution to Program Qualifications
Course Contribution to Program Qualifications
No
Program Qualification
Contribution Level
1
2
3
4
5
1
Know about histology of basic tissue, basic embryological development, mechanisms of routine micro-preparation techniques, and the basics of active use of a microscope
X
2
Have knowledge of ethics of research and the rules of documenting a research
3
Can relay information about basic histology-embryology
4
Can work on tissue preservation, tissue dye and do microscopic examination.
X
5
Can do literature review
X
6
Can attend both clinical and nonclinical studies and make proposals for methods.
X
7
Can document outcomes.
X
8
Express themselves by using resources in Histology-Embryology and in the areas of histotechnical science, and communication techniques.
X
9
Improve their critical thinking, and can create strategies.
X
10
Behave with an ethical concern in both clinical and nonclinical preparation.
11
Have the awareness of business ethics and create a secure working environment, evaluate the contributors.
12
Exhibit scientific assets in national or international scientific meetings using foreign languages besides their mother language.
13
Promote interdisciplinary cooperation with the consciousness of social responsibility and remain true to professional secrecy, create final decleration within the framework of the quality.
