Course Supervisor: Prof. Dr Esra Çağavi, Course LaboAssistants: Laboratory supervisor from Research Institute for Health Sciences and Technologies (SABITA),
Aim
The aim of the Laboratory Rotation-II course is for Master’s level students to observe and be able to explain the basic technical approaches in laboratory application that will benefit their thesis work and understanding of the scope of different laboratory disciplines. By rotating in different labs every week, students are aimed to be exposed to the basic molecular biology techniques, proteomics and genomics applications, FACS, primary and cell line culture applications, transgenic animal models, electrophysiology, neuroscience and cancer research applications, basic protein and gene expression analysis.
Course Content
This course contains; Electroencephalography (EEG) and its use in scientific research,Electroencephalography (EEG) application and practice,Flourescence activated cell sorting (FACS) method,Functional Neurology Applications,Primary cells and cell line culture applications,Experimental and transgenic animal models and applications
,Evaluation of Laboratory Notebooks for the Midterm Exam,Drug screening and cell survival tests,Electrophysiology and patch-clamp method,Gene expression Analysis in Molecular Biology,Cancer Biology Lab and Applicaitons,Histology and cryosectioning technique,Protein Analysis in Molecular Biology,Evaluation of Laboratory Notebooks for the Final Exam.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Explains the culture methods for differen cell type, experimental transgenic animal models, basic neuroscience application as EEG and electrophsiology, basic approaches in cancer, cell survival analysis, FACS and tissue sectioning practices.
14, 17, 3, 9
A, E
Explain principles of different experimental techniques.
14, 17, 9
A, E
Define the principles of basic research laboratory equipments.
14, 17, 9
A, E
Explains how to process and interpret scientific data at the basic level.
14, 17, 9
A, E
Teaching Methods:
14: Self Study Method, 17: Experimental Technique, 3: Problem Baded Learning Model, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, E: Homework
Course Outline
Order
Subjects
Preliminary Work
1
Electroencephalography (EEG) and its use in scientific research
Self-study
2
Electroencephalography (EEG) application and practice
Self-study
3
Flourescence activated cell sorting (FACS) method
Self-study
4
Functional Neurology Applications
Self-study
5
Primary cells and cell line culture applications
Self-study
6
Experimental and transgenic animal models and applications
7
Evaluation of Laboratory Notebooks for the Midterm Exam
8
Drug screening and cell survival tests
9
Electrophysiology and patch-clamp method
10
Gene expression Analysis in Molecular Biology
11
Cancer Biology Lab and Applicaitons
12
Histology and cryosectioning technique
13
Protein Analysis in Molecular Biology
14
Evaluation of Laboratory Notebooks for the Final Exam
Knows the biochemical macromolecular metabolism. Applies and advances the knowlegde.
2
Knows the principles of routine and research oriented biochemical technologies.
X
3
Practical Laboratory Applications (Sampling conditions of various body fluids and tissue samples in health and disease states, reasons of analytical errors, storage conditions of samples,working principles of technologies used in routine and research based studies, determinig reference values.)
X
4
Knows basic biostatistics methods, applies to experimental study results and evaluates.
5
Knows how to use specific instruments in biochemistry
X
6
Can access printeed and electronic literature and able to follow the advancements in biochemistry and related field.
7
Can integrate and interpret the knowledge gained in biochemistry with knowledge obtained in other fields and can generate new information.
X
8
Independantly applies his/her theoretical knowledge, critical thinking skills to the area of study, evaluates the results and writes a report.
9
Defines a technical or scientific problem either with the helpof an advisor or independently, can come up with proposals and solve when needed.
X
10
Take on responsibility in collborative studies, contributes and work in harmony.
11
Follows national and international literature in Biochemistry, presents the work in seminars or publishes in journals.
X
12
Can evaluate and teach biochemical data collection, interpretation, application and decleration recpecting cultural, scientific, social and ethical values.
13
Take responsibility in managing, evaluate the results according to the context of quality processes.
Assessment Methods
Contribution Level
Absolute Evaluation
Rate of Midterm Exam to Success
50
Rate of Final Exam to Success
50
Total
100
ECTS / Workload Table
Activities
Number of
Duration(Hour)
Total Workload(Hour)
Course Hours
14
3
42
Guided Problem Solving
6
3
18
Resolution of Homework Problems and Submission as a Report
14
5
70
Term Project
0
0
0
Presentation of Project / Seminar
0
0
0
Quiz
0
0
0
Midterm Exam
1
20
20
General Exam
1
20
20
Performance Task, Maintenance Plan
0
0
0
Total Workload(Hour)
170
Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(170/30)
6
ECTS of the course: 30 hours of work is counted as 1 ECTS credit.
Course Supervisor: Prof. Dr Esra Çağavi, Course LaboAssistants: Laboratory supervisor from Research Institute for Health Sciences and Technologies (SABITA),
Aim
The aim of the Laboratory Rotation-II course is for Master’s level students to observe and be able to explain the basic technical approaches in laboratory application that will benefit their thesis work and understanding of the scope of different laboratory disciplines. By rotating in different labs every week, students are aimed to be exposed to the basic molecular biology techniques, proteomics and genomics applications, FACS, primary and cell line culture applications, transgenic animal models, electrophysiology, neuroscience and cancer research applications, basic protein and gene expression analysis.
Course Content
This course contains; Electroencephalography (EEG) and its use in scientific research,Electroencephalography (EEG) application and practice,Flourescence activated cell sorting (FACS) method,Functional Neurology Applications,Primary cells and cell line culture applications,Experimental and transgenic animal models and applications
,Evaluation of Laboratory Notebooks for the Midterm Exam,Drug screening and cell survival tests,Electrophysiology and patch-clamp method,Gene expression Analysis in Molecular Biology,Cancer Biology Lab and Applicaitons,Histology and cryosectioning technique,Protein Analysis in Molecular Biology,Evaluation of Laboratory Notebooks for the Final Exam.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Explains the culture methods for differen cell type, experimental transgenic animal models, basic neuroscience application as EEG and electrophsiology, basic approaches in cancer, cell survival analysis, FACS and tissue sectioning practices.
14, 17, 3, 9
A, E
Explain principles of different experimental techniques.
14, 17, 9
A, E
Define the principles of basic research laboratory equipments.
14, 17, 9
A, E
Explains how to process and interpret scientific data at the basic level.
14, 17, 9
A, E
Teaching Methods:
14: Self Study Method, 17: Experimental Technique, 3: Problem Baded Learning Model, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, E: Homework
Course Outline
Order
Subjects
Preliminary Work
1
Electroencephalography (EEG) and its use in scientific research
Self-study
2
Electroencephalography (EEG) application and practice
Self-study
3
Flourescence activated cell sorting (FACS) method
Self-study
4
Functional Neurology Applications
Self-study
5
Primary cells and cell line culture applications
Self-study
6
Experimental and transgenic animal models and applications
7
Evaluation of Laboratory Notebooks for the Midterm Exam
8
Drug screening and cell survival tests
9
Electrophysiology and patch-clamp method
10
Gene expression Analysis in Molecular Biology
11
Cancer Biology Lab and Applicaitons
12
Histology and cryosectioning technique
13
Protein Analysis in Molecular Biology
14
Evaluation of Laboratory Notebooks for the Final Exam
Knows the biochemical macromolecular metabolism. Applies and advances the knowlegde.
2
Knows the principles of routine and research oriented biochemical technologies.
X
3
Practical Laboratory Applications (Sampling conditions of various body fluids and tissue samples in health and disease states, reasons of analytical errors, storage conditions of samples,working principles of technologies used in routine and research based studies, determinig reference values.)
X
4
Knows basic biostatistics methods, applies to experimental study results and evaluates.
5
Knows how to use specific instruments in biochemistry
X
6
Can access printeed and electronic literature and able to follow the advancements in biochemistry and related field.
7
Can integrate and interpret the knowledge gained in biochemistry with knowledge obtained in other fields and can generate new information.
X
8
Independantly applies his/her theoretical knowledge, critical thinking skills to the area of study, evaluates the results and writes a report.
9
Defines a technical or scientific problem either with the helpof an advisor or independently, can come up with proposals and solve when needed.
X
10
Take on responsibility in collborative studies, contributes and work in harmony.
11
Follows national and international literature in Biochemistry, presents the work in seminars or publishes in journals.
X
12
Can evaluate and teach biochemical data collection, interpretation, application and decleration recpecting cultural, scientific, social and ethical values.
13
Take responsibility in managing, evaluate the results according to the context of quality processes.
