Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| RESEARCH TECHNICS in BIOCHEMISTY I | - | Spring Semester | 1+2 | 2 | 5 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Second Cycle (Master's Degree) |
| Course Type | Elective |
| Course Coordinator | |
| Name of Lecturer(s) | Prof.Dr. Neslin EMEKLİ, Prof.Dr. Türkan YİĞİTBAŞI, Assoc.Prof. Sultan Sibel ERDEM, Assist.Prof. Gözde ÜLFER, Assoc.Prof. Mukaddes ÇOLAKOĞULLARI |
| Assistant(s) | Prof.Dr.Nesrin Emekli, Assoc. Prof. Dr.Türkan Yiğitbaşı, Ass. Prof .Dr.Sibel Erdem |
| Aim | Introduction to the devices used in biochemical research |
| Course Content | This course contains; Purification techniques in the blood and the cell,Preparatif and analitic purification techniques,Principle of chromatograpic seperation,Chromatographic analysis grouped according to their mechanism of seperation,Chromatographic analysis which are grouped by application format,The principle of electrophoretic seperation,Electrophoresis according to the supporting media,The principle of immunologic methods,Immunoassays,Unlabeled and labeled immunoassay,Nucleic acid based techniques,Immunohistochemical techniques,Automated analysis,Quality managementin in the laboratory. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| 1-Can discuss protein and enzyme purification methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.1.Discuss the preparative and analytical purification methods of enzymes and proteins. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.3.Distinguish between extraction, precipitation and ultracentrifugal separation methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.5.Discuss the storage conditions of the purified molecule. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2-Kromatografik ayırma yöntemlerini tartışabilir. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.1.Discuss the importance and working principles of adsorption chromatography. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.2.Describes distribution and ion exchange chromatography. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.3.Compares gel filtration and affinity chromatography. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.4.Explains the separation according to phase types in chromatographic analysis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3-Discuss electrophoretic separation methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.1.Explains sample loading, staining and imaging in electrophoresis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.2.Describes SDS-Polyacrylamide gel electrophoresis (PAGE). | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.3.Explains the usage areas of agorose electrophoresis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.4.Explains the usage areas of capillary electrophoresis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.5.Explains isoelectric focusing in electrophoresis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.6.Explains two-dimensional electrophoresis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4-Can discuss immunochemical and radioimmunochemical methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.1.Explains immunoelectrophoresis and its principles. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.2.Explains radial immunodiffusion and bidirectional immunodiffusion methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.3.Describes rocket immunoelectrophoresis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.4.Describes the Radioimmunassay (RIA) method. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.5.Describes the Immunoradioimmunoassay (IRMA) method. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.6.Describes and applies the enzyme linked immunoassay (ELISA) method. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.7.Describes the chemiluminescent immunoassay (CL) method. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.8.Describes the electrochemiluminescent immonoassay (ECL) method. | 10, 16, 19, 6, 9 | A, D, E |
| 5-Describes the polymerase chain reaction (PCR). | 10, 16, 19, 6, 9 | A, D, E |
| 5.1.Explains the conditions for propagation of nucleic acids. | 10, 16, 19, 6, 9 | A, D, E |
| 5.3.Explains the separation of DNA chains at high temperatures, the binding of synthetic oligonucleotides to target DNA, the elongation of the chain and the importance of ambient temperature in this cycle. | 10, 16, 19, 6, 9 | A, D, E |
| 5.4.Discuss the areas where PCR technology is used. | 10, 16, 19, 6, 9 | A, D, E |
| 6-Discuss the immunohistochemical methods. | 10, 16, 19, 6, 9 | A, D, E |
| 6.1.Explains the immunohistochemical method used in cells and body fluids. | 10, 16, 19, 6, 9 | A, D, E |
| 6.2.Explains how samples are taken in the immunohistochemical method. | 10, 16, 19, 6, 9 | A, D, E |
| 6.3.Describes the dyes used in the immunohistochemical method. | 10, 16, 19, 6, 9 | A, D, E |
| 6.4.Discuss the importance of immunohistochemical methods in diagnosis and treatment. | 10, 16, 19, 6, 9 | A, D, E |
| 7-Discuss automatic spectrophotometers (Autoanalyzer). | 10, 16, 19, 6, 9 | A, D, E |
| 7.1.Explains how to place samples and reagents into the autoanalyzer. | 10, 16, 19, 6, 9 | A, D, E |
| 7.2.Explains the chemical reaction phases in the autoanalyzer. | 10, 16, 19, 6, 9 | A, D, E |
| 7.3.Explains how the measurement phase is carried out in the autoanalyzer. | 10, 16, 19, 6, 9 | A, D, E |
| 1.2. Analyzes which path to follow for purification. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.4. Analyzes which methods will be used in the separation of small molecules at various stages of purification. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.4. Query using PCR technology fields | 10, 16, 19, 6, 9 | A, D, E |
| 7.4. Analyzes the results in the autoanalyzer. | 10, 16, 19, 6, 9 | A, D, E |
| Teaching Methods: | 10: Discussion Method, 11: Demonstration Method, 16: Question - Answer Technique, 19: Brainstorming Technique, 6: Experiential Learning, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, D: Oral Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | Purification techniques in the blood and the cell | 1,2,3,4 |
| 2 | Preparatif and analitic purification techniques | 1,2,3,4 |
| 3 | Principle of chromatograpic seperation | 1,2,3,4 |
| 4 | Chromatographic analysis grouped according to their mechanism of seperation | 1,2,3,4 |
| 5 | Chromatographic analysis which are grouped by application format | 1,2,3,4 |
| 6 | The principle of electrophoretic seperation | 1,2,3,4 |
| 7 | Electrophoresis according to the supporting media | 1,2,3,4 |
| 8 | The principle of immunologic methods | 1,2,3,4 |
| 9 | Immunoassays | 1,2,3,4 |
| 10 | Unlabeled and labeled immunoassay | 1,2,3,4 |
| 11 | Nucleic acid based techniques | 1,2,3,4 |
| 12 | Immunohistochemical techniques | 1,2,3,4 |
| 13 | Automated analysis | 1,2,3,4 |
| 14 | Quality managementin in the laboratory | 1,2,3,4 |
| Resources |
| Biochemistry Lab Book For Students(Doç.Dr.Türkan Yiğitbaşı,Prof.Dr.Nesrin Emekli) Medipol Üniversitesi Yayınları |
| 1.Klinik biyokimya, N emekli, t.yiğitbaşı, İstanbul Medipol Üniversitesi Yayınları, Dağıtım nobel tıp kitapevleri 2015 2.Henry's Clinical Diagnosis and Management by Laboratory Methods. Elsevier Saunders, 2011 3.Recep Sütçü; Moleküler tanı teknikleri içinde: Klinik Biyokimya, İstanbul Medipol Üniversitesi Yayınları, s.73-85 4.Nihal Büyükuslu:Biyokimyada ileri araştırma teknikleri: İçinde;Klinik Biyokimya İst.Medipol Üniv. Yayınları 2015 |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Knows the biochemical macromolecular metabolism. Applies and advances the knowlegde. | X | |||||
| 2 | Knows the principles of routine and research oriented biochemical technologies. | X | |||||
| 3 | Practical Laboratory Applications (Sampling conditions of various body fluids and tissue samples in health and disease states, reasons of analytical errors, storage conditions of samples,working principles of technologies used in routine and research based studies, determinig reference values.) | X | |||||
| 4 | Knows basic biostatistics methods, applies to experimental study results and evaluates. | ||||||
| 5 | Knows how to use specific instruments in biochemistry | X | |||||
| 6 | Can access printeed and electronic literature and able to follow the advancements in biochemistry and related field. | X | |||||
| 7 | Can integrate and interpret the knowledge gained in biochemistry with knowledge obtained in other fields and can generate new information. | ||||||
| 8 | Independantly applies his/her theoretical knowledge, critical thinking skills to the area of study, evaluates the results and writes a report. | X | |||||
| 9 | Defines a technical or scientific problem either with the helpof an advisor or independently, can come up with proposals and solve when needed. | X | |||||
| 10 | Take on responsibility in collborative studies, contributes and work in harmony. | X | |||||
| 11 | Follows national and international literature in Biochemistry, presents the work in seminars or publishes in journals. | X | |||||
| 12 | Can evaluate and teach biochemical data collection, interpretation, application and decleration recpecting cultural, scientific, social and ethical values. | X | |||||
| 13 | Take responsibility in managing, evaluate the results according to the context of quality processes. | X | |||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |
| ECTS / Workload Table | ||||||
| Activities | Number of | Duration(Hour) | Total Workload(Hour) | |||
| Course Hours | 14 | 2 | 28 | |||
| Guided Problem Solving | 14 | 2 | 28 | |||
| Resolution of Homework Problems and Submission as a Report | 5 | 5 | 25 | |||
| Term Project | 5 | 3 | 15 | |||
| Presentation of Project / Seminar | 2 | 1 | 2 | |||
| Quiz | 0 | 0 | 0 | |||
| Midterm Exam | 1 | 20 | 20 | |||
| General Exam | 1 | 30 | 30 | |||
| Performance Task, Maintenance Plan | 0 | 0 | 0 | |||
| Total Workload(Hour) | 148 | |||||
| Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(148/30) | 5 | |||||
| ECTS of the course: 30 hours of work is counted as 1 ECTS credit. | ||||||
Detail Informations of the Course
Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| RESEARCH TECHNICS in BIOCHEMISTY I | - | Spring Semester | 1+2 | 2 | 5 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Second Cycle (Master's Degree) |
| Course Type | Elective |
| Course Coordinator | |
| Name of Lecturer(s) | Prof.Dr. Neslin EMEKLİ, Prof.Dr. Türkan YİĞİTBAŞI, Assoc.Prof. Sultan Sibel ERDEM, Assist.Prof. Gözde ÜLFER, Assoc.Prof. Mukaddes ÇOLAKOĞULLARI |
| Assistant(s) | Prof.Dr.Nesrin Emekli, Assoc. Prof. Dr.Türkan Yiğitbaşı, Ass. Prof .Dr.Sibel Erdem |
| Aim | Introduction to the devices used in biochemical research |
| Course Content | This course contains; Purification techniques in the blood and the cell,Preparatif and analitic purification techniques,Principle of chromatograpic seperation,Chromatographic analysis grouped according to their mechanism of seperation,Chromatographic analysis which are grouped by application format,The principle of electrophoretic seperation,Electrophoresis according to the supporting media,The principle of immunologic methods,Immunoassays,Unlabeled and labeled immunoassay,Nucleic acid based techniques,Immunohistochemical techniques,Automated analysis,Quality managementin in the laboratory. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| 1-Can discuss protein and enzyme purification methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.1.Discuss the preparative and analytical purification methods of enzymes and proteins. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.3.Distinguish between extraction, precipitation and ultracentrifugal separation methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.5.Discuss the storage conditions of the purified molecule. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2-Kromatografik ayırma yöntemlerini tartışabilir. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.1.Discuss the importance and working principles of adsorption chromatography. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.2.Describes distribution and ion exchange chromatography. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.3.Compares gel filtration and affinity chromatography. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.4.Explains the separation according to phase types in chromatographic analysis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3-Discuss electrophoretic separation methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.1.Explains sample loading, staining and imaging in electrophoresis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.2.Describes SDS-Polyacrylamide gel electrophoresis (PAGE). | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.3.Explains the usage areas of agorose electrophoresis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.4.Explains the usage areas of capillary electrophoresis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.5.Explains isoelectric focusing in electrophoresis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.6.Explains two-dimensional electrophoresis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4-Can discuss immunochemical and radioimmunochemical methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.1.Explains immunoelectrophoresis and its principles. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.2.Explains radial immunodiffusion and bidirectional immunodiffusion methods. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.3.Describes rocket immunoelectrophoresis. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.4.Describes the Radioimmunassay (RIA) method. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.5.Describes the Immunoradioimmunoassay (IRMA) method. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.6.Describes and applies the enzyme linked immunoassay (ELISA) method. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.7.Describes the chemiluminescent immunoassay (CL) method. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.8.Describes the electrochemiluminescent immonoassay (ECL) method. | 10, 16, 19, 6, 9 | A, D, E |
| 5-Describes the polymerase chain reaction (PCR). | 10, 16, 19, 6, 9 | A, D, E |
| 5.1.Explains the conditions for propagation of nucleic acids. | 10, 16, 19, 6, 9 | A, D, E |
| 5.3.Explains the separation of DNA chains at high temperatures, the binding of synthetic oligonucleotides to target DNA, the elongation of the chain and the importance of ambient temperature in this cycle. | 10, 16, 19, 6, 9 | A, D, E |
| 5.4.Discuss the areas where PCR technology is used. | 10, 16, 19, 6, 9 | A, D, E |
| 6-Discuss the immunohistochemical methods. | 10, 16, 19, 6, 9 | A, D, E |
| 6.1.Explains the immunohistochemical method used in cells and body fluids. | 10, 16, 19, 6, 9 | A, D, E |
| 6.2.Explains how samples are taken in the immunohistochemical method. | 10, 16, 19, 6, 9 | A, D, E |
| 6.3.Describes the dyes used in the immunohistochemical method. | 10, 16, 19, 6, 9 | A, D, E |
| 6.4.Discuss the importance of immunohistochemical methods in diagnosis and treatment. | 10, 16, 19, 6, 9 | A, D, E |
| 7-Discuss automatic spectrophotometers (Autoanalyzer). | 10, 16, 19, 6, 9 | A, D, E |
| 7.1.Explains how to place samples and reagents into the autoanalyzer. | 10, 16, 19, 6, 9 | A, D, E |
| 7.2.Explains the chemical reaction phases in the autoanalyzer. | 10, 16, 19, 6, 9 | A, D, E |
| 7.3.Explains how the measurement phase is carried out in the autoanalyzer. | 10, 16, 19, 6, 9 | A, D, E |
| 1.2. Analyzes which path to follow for purification. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.4. Analyzes which methods will be used in the separation of small molecules at various stages of purification. | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.4. Query using PCR technology fields | 10, 16, 19, 6, 9 | A, D, E |
| 7.4. Analyzes the results in the autoanalyzer. | 10, 16, 19, 6, 9 | A, D, E |
| Teaching Methods: | 10: Discussion Method, 11: Demonstration Method, 16: Question - Answer Technique, 19: Brainstorming Technique, 6: Experiential Learning, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, D: Oral Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | Purification techniques in the blood and the cell | 1,2,3,4 |
| 2 | Preparatif and analitic purification techniques | 1,2,3,4 |
| 3 | Principle of chromatograpic seperation | 1,2,3,4 |
| 4 | Chromatographic analysis grouped according to their mechanism of seperation | 1,2,3,4 |
| 5 | Chromatographic analysis which are grouped by application format | 1,2,3,4 |
| 6 | The principle of electrophoretic seperation | 1,2,3,4 |
| 7 | Electrophoresis according to the supporting media | 1,2,3,4 |
| 8 | The principle of immunologic methods | 1,2,3,4 |
| 9 | Immunoassays | 1,2,3,4 |
| 10 | Unlabeled and labeled immunoassay | 1,2,3,4 |
| 11 | Nucleic acid based techniques | 1,2,3,4 |
| 12 | Immunohistochemical techniques | 1,2,3,4 |
| 13 | Automated analysis | 1,2,3,4 |
| 14 | Quality managementin in the laboratory | 1,2,3,4 |
| Resources |
| Biochemistry Lab Book For Students(Doç.Dr.Türkan Yiğitbaşı,Prof.Dr.Nesrin Emekli) Medipol Üniversitesi Yayınları |
| 1.Klinik biyokimya, N emekli, t.yiğitbaşı, İstanbul Medipol Üniversitesi Yayınları, Dağıtım nobel tıp kitapevleri 2015 2.Henry's Clinical Diagnosis and Management by Laboratory Methods. Elsevier Saunders, 2011 3.Recep Sütçü; Moleküler tanı teknikleri içinde: Klinik Biyokimya, İstanbul Medipol Üniversitesi Yayınları, s.73-85 4.Nihal Büyükuslu:Biyokimyada ileri araştırma teknikleri: İçinde;Klinik Biyokimya İst.Medipol Üniv. Yayınları 2015 |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Knows the biochemical macromolecular metabolism. Applies and advances the knowlegde. | X | |||||
| 2 | Knows the principles of routine and research oriented biochemical technologies. | X | |||||
| 3 | Practical Laboratory Applications (Sampling conditions of various body fluids and tissue samples in health and disease states, reasons of analytical errors, storage conditions of samples,working principles of technologies used in routine and research based studies, determinig reference values.) | X | |||||
| 4 | Knows basic biostatistics methods, applies to experimental study results and evaluates. | ||||||
| 5 | Knows how to use specific instruments in biochemistry | X | |||||
| 6 | Can access printeed and electronic literature and able to follow the advancements in biochemistry and related field. | X | |||||
| 7 | Can integrate and interpret the knowledge gained in biochemistry with knowledge obtained in other fields and can generate new information. | ||||||
| 8 | Independantly applies his/her theoretical knowledge, critical thinking skills to the area of study, evaluates the results and writes a report. | X | |||||
| 9 | Defines a technical or scientific problem either with the helpof an advisor or independently, can come up with proposals and solve when needed. | X | |||||
| 10 | Take on responsibility in collborative studies, contributes and work in harmony. | X | |||||
| 11 | Follows national and international literature in Biochemistry, presents the work in seminars or publishes in journals. | X | |||||
| 12 | Can evaluate and teach biochemical data collection, interpretation, application and decleration recpecting cultural, scientific, social and ethical values. | X | |||||
| 13 | Take responsibility in managing, evaluate the results according to the context of quality processes. | X | |||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |