Course Detail
Ders Detayı
Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| CLINICAL ENZYMOLOGY | BYKD1214250 | Spring Semester | 2+2 | 3 | 8 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Third Cycle (Doctorate Degree) |
| Course Type | Elective |
| Course Coordinator | Assist.Prof. Gözde ÜLFER |
| Name of Lecturer(s) | Prof.Dr. Neslin EMEKLİ, Prof.Dr. Türkan YİĞİTBAŞI, Assist.Prof. Gözde ÜLFER, Prof.Dr. Sultan Sibel ERDEM, Assist.Prof. Çağrı ÇAKICI, Assoc.Prof. Derya CANSIZ |
| Assistant(s) | |
| Aim | To gain advanced knowledge about enzymes and enzymatic reactions and to discuss potential enzyme-based drug development |
| Course Content | This course contains; 1-General properties of the enzymes,2-Classification of the enzymes,3-Isoenzymes,4-Cofactors,5-Factors affectşng enzyme reactions,6-Enzyme Kinetics,7-Enzyme inhibitors,8-Catalytic activity of enzymes and drugs,9-Analysis of enzyme activity,10-Acid and ve alkali phosphatase (3.1.3.2 and 3.1.3.1),11-ACE (3.4.15.1),12-Laktate dehydrogenase (EC 1.1.1.27),13-5’Nucleotidase (EC 3.1.3.5),14-Other enzymes. |
| Course Learning Outcomes | Teaching Methods | Assessment Methods |
| 1-Explains general properties of the enzymes | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.1.Explains catalyzer effect of the enzymes | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.2.Explain substrate specificity property of the enzymes | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.3.Explains the molecular basis of the catalytic property | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.4.Explain isoenzymes and related pathological conditions | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.5.Explain coenzymes and cofactors | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.6.Explain half-life of enzymes in blood | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2-Explain classification of the enzymes | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.1.Explain main and sub groups of enzymes | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.2.Explain specificity of carbon bonds for classification | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3-Question reactions of the enzymes | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.1.Explain the factors affecting enzymatic reactions | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.2.Explain Michaelis-Menten constant and its importance for enzymes | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.3.Explain how to draw Lineweaver-Burk linear | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.4.Question Km value | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4-Question enzyme activators and inhibitors | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.1.Explain competitive inhibition | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.2.Explain uncompetitive inhibition | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.4.Develop new ideas for the development of enzyme-based drugs | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5-Discuss enzymes that are responsible for energy conversion from nutrition | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.1.Describe mechanism action of enzymes in the first main group | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.2.Describe mechanism action of enzymes in the second main group | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.3.Question mechanism action of enzymes in the third main group | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.4.Question mechanism action of enzymes in the other main groups | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.3. Explain how to isolate enzymes from blood, cell and from other body fluids | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.3. Explain noncompetitive inhibition | 10, 11, 16, 19, 6, 9 | A, D, E |
| Teaching Methods: | 10: Discussion Method, 11: Demonstration Method, 16: Question - Answer Technique, 19: Brainstorming Technique, 6: Experiential Learning, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, D: Oral Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | 1-General properties of the enzymes | 1, 2, 3, 4, 5 |
| 2 | 2-Classification of the enzymes | 1, 2, 3, 4, 5 |
| 3 | 3-Isoenzymes | 1, 2, 3, 4, 5 |
| 4 | 4-Cofactors | 1, 2, 3, 4, 5 |
| 5 | 5-Factors affectşng enzyme reactions | 1, 2, 3, 4, 5 |
| 6 | 6-Enzyme Kinetics | 1, 2, 3, 4, 5 |
| 7 | 7-Enzyme inhibitors | 1, 2, 3, 4, 5 |
| 8 | 8-Catalytic activity of enzymes and drugs | 1, 2, 3, 4, 5 |
| 9 | 9-Analysis of enzyme activity | 1, 2, 3, 4, 5 |
| 10 | 10-Acid and ve alkali phosphatase (3.1.3.2 and 3.1.3.1) | 1, 2, 3, 4, 5 |
| 11 | 11-ACE (3.4.15.1) | 1, 2, 3, 4, 5 |
| 12 | 12-Laktate dehydrogenase (EC 1.1.1.27) | 1, 2, 3, 4, 5 |
| 13 | 13-5’Nucleotidase (EC 3.1.3.5) | 1, 2, 3, 4, 5 |
| 14 | 14-Other enzymes | 1, 2, 3, 4, 5 |
| Resources |
| Published books of Prof. Dr. Nesrin Emekli ve Assoc Prof. Dr. Türkan Yiğitbaşı |
| Henry's Clinical diagnosis and Managemen by laboratory methods Lehninger Biochemistry Kaplan & Pesce Clinical Biochemistry |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Defines macro and micromolecules in cell and body fluids at an advanced level. | ||||||
| 2 | Knows how to store and protect the material to be analyzed. | ||||||
| 3 | Knows how to identify molecules found in cells and body fluids. | ||||||
| 4 | Knows the principles of technologies to be used for routine and research purposes. | ||||||
| 5 | Knows how to evaluate the results of the study, interprets and questions. | ||||||
| 6 | Knows how to use field-specific tools and equipment | ||||||
| 7 | Knows how to access and interpret field-specific resources and information | ||||||
| 8 | Shares information with disciplines related to the field | ||||||
| 9 | Combines theoretical and factual knowledge with critical thoughts, conducts independent studies, evaluates the results, compares them with the literature, and gets them accepted by authors and scientific journals. | ||||||
| 10 | Solves a scientific and technical problem, knows where to get help. | ||||||
| 11 | Takes responsibility in joint scientific studies, contributes and works in harmony. | ||||||
| 12 | Participates in meetings related to the field of biochemistry, presents papers, participates in discussions and congresses, publishes studies in reputable journals specific to the field. | ||||||
| 13 | Collects, interprets, applies, announces data related to the field of biochemistry, conducts social and cultural studies, and supervises these studies scientifically and socially. | ||||||
| 14 | Can take part in management activities, evaluate the results obtained to improve quality | ||||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |
| ECTS / Workload Table | ||||||
| Activities | Number of | Duration(Hour) | Total Workload(Hour) | |||
| Course Hours | 14 | 2 | 28 | |||
| Guided Problem Solving | 14 | 12 | 168 | |||
| Resolution of Homework Problems and Submission as a Report | 0 | 0 | 0 | |||
| Term Project | 0 | 0 | 0 | |||
| Presentation of Project / Seminar | 0 | 0 | 0 | |||
| Quiz | 0 | 0 | 0 | |||
| Midterm Exam | 0 | 0 | 0 | |||
| General Exam | 0 | 0 | 0 | |||
| Performance Task, Maintenance Plan | 0 | 0 | 0 | |||
| Total Workload(Hour) | 196 | |||||
| Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(196/30) | 7 | |||||
| ECTS of the course: 30 hours of work is counted as 1 ECTS credit. | ||||||
Detail Informations of the Course
Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| CLINICAL ENZYMOLOGY | BYKD1214250 | Spring Semester | 2+2 | 3 | 8 |
| Course Program |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | Turkish |
| Course Level | Third Cycle (Doctorate Degree) |
| Course Type | Elective |
| Course Coordinator | Assist.Prof. Gözde ÜLFER |
| Name of Lecturer(s) | Prof.Dr. Neslin EMEKLİ, Prof.Dr. Türkan YİĞİTBAŞI, Assist.Prof. Gözde ÜLFER, Prof.Dr. Sultan Sibel ERDEM, Assist.Prof. Çağrı ÇAKICI, Assoc.Prof. Derya CANSIZ |
| Assistant(s) | |
| Aim | To gain advanced knowledge about enzymes and enzymatic reactions and to discuss potential enzyme-based drug development |
| Course Content | This course contains; 1-General properties of the enzymes,2-Classification of the enzymes,3-Isoenzymes,4-Cofactors,5-Factors affectşng enzyme reactions,6-Enzyme Kinetics,7-Enzyme inhibitors,8-Catalytic activity of enzymes and drugs,9-Analysis of enzyme activity,10-Acid and ve alkali phosphatase (3.1.3.2 and 3.1.3.1),11-ACE (3.4.15.1),12-Laktate dehydrogenase (EC 1.1.1.27),13-5’Nucleotidase (EC 3.1.3.5),14-Other enzymes. |
| Course Learning Outcomes | Teaching Methods | Assessment Methods |
| 1-Explains general properties of the enzymes | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.1.Explains catalyzer effect of the enzymes | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.2.Explain substrate specificity property of the enzymes | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.3.Explains the molecular basis of the catalytic property | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.4.Explain isoenzymes and related pathological conditions | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.5.Explain coenzymes and cofactors | 10, 11, 16, 19, 6, 9 | A, D, E |
| 1.6.Explain half-life of enzymes in blood | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2-Explain classification of the enzymes | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.1.Explain main and sub groups of enzymes | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.2.Explain specificity of carbon bonds for classification | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3-Question reactions of the enzymes | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.1.Explain the factors affecting enzymatic reactions | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.2.Explain Michaelis-Menten constant and its importance for enzymes | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.3.Explain how to draw Lineweaver-Burk linear | 10, 11, 16, 19, 6, 9 | A, D, E |
| 3.4.Question Km value | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4-Question enzyme activators and inhibitors | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.1.Explain competitive inhibition | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.2.Explain uncompetitive inhibition | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.4.Develop new ideas for the development of enzyme-based drugs | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5-Discuss enzymes that are responsible for energy conversion from nutrition | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.1.Describe mechanism action of enzymes in the first main group | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.2.Describe mechanism action of enzymes in the second main group | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.3.Question mechanism action of enzymes in the third main group | 10, 11, 16, 19, 6, 9 | A, D, E |
| 5.4.Question mechanism action of enzymes in the other main groups | 10, 11, 16, 19, 6, 9 | A, D, E |
| 2.3. Explain how to isolate enzymes from blood, cell and from other body fluids | 10, 11, 16, 19, 6, 9 | A, D, E |
| 4.3. Explain noncompetitive inhibition | 10, 11, 16, 19, 6, 9 | A, D, E |
| Teaching Methods: | 10: Discussion Method, 11: Demonstration Method, 16: Question - Answer Technique, 19: Brainstorming Technique, 6: Experiential Learning, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, D: Oral Exam, E: Homework |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | 1-General properties of the enzymes | 1, 2, 3, 4, 5 |
| 2 | 2-Classification of the enzymes | 1, 2, 3, 4, 5 |
| 3 | 3-Isoenzymes | 1, 2, 3, 4, 5 |
| 4 | 4-Cofactors | 1, 2, 3, 4, 5 |
| 5 | 5-Factors affectşng enzyme reactions | 1, 2, 3, 4, 5 |
| 6 | 6-Enzyme Kinetics | 1, 2, 3, 4, 5 |
| 7 | 7-Enzyme inhibitors | 1, 2, 3, 4, 5 |
| 8 | 8-Catalytic activity of enzymes and drugs | 1, 2, 3, 4, 5 |
| 9 | 9-Analysis of enzyme activity | 1, 2, 3, 4, 5 |
| 10 | 10-Acid and ve alkali phosphatase (3.1.3.2 and 3.1.3.1) | 1, 2, 3, 4, 5 |
| 11 | 11-ACE (3.4.15.1) | 1, 2, 3, 4, 5 |
| 12 | 12-Laktate dehydrogenase (EC 1.1.1.27) | 1, 2, 3, 4, 5 |
| 13 | 13-5’Nucleotidase (EC 3.1.3.5) | 1, 2, 3, 4, 5 |
| 14 | 14-Other enzymes | 1, 2, 3, 4, 5 |
| Resources |
| Published books of Prof. Dr. Nesrin Emekli ve Assoc Prof. Dr. Türkan Yiğitbaşı |
| Henry's Clinical diagnosis and Managemen by laboratory methods Lehninger Biochemistry Kaplan & Pesce Clinical Biochemistry |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Defines macro and micromolecules in cell and body fluids at an advanced level. | ||||||
| 2 | Knows how to store and protect the material to be analyzed. | ||||||
| 3 | Knows how to identify molecules found in cells and body fluids. | ||||||
| 4 | Knows the principles of technologies to be used for routine and research purposes. | ||||||
| 5 | Knows how to evaluate the results of the study, interprets and questions. | ||||||
| 6 | Knows how to use field-specific tools and equipment | ||||||
| 7 | Knows how to access and interpret field-specific resources and information | ||||||
| 8 | Shares information with disciplines related to the field | ||||||
| 9 | Combines theoretical and factual knowledge with critical thoughts, conducts independent studies, evaluates the results, compares them with the literature, and gets them accepted by authors and scientific journals. | ||||||
| 10 | Solves a scientific and technical problem, knows where to get help. | ||||||
| 11 | Takes responsibility in joint scientific studies, contributes and works in harmony. | ||||||
| 12 | Participates in meetings related to the field of biochemistry, presents papers, participates in discussions and congresses, publishes studies in reputable journals specific to the field. | ||||||
| 13 | Collects, interprets, applies, announces data related to the field of biochemistry, conducts social and cultural studies, and supervises these studies scientifically and socially. | ||||||
| 14 | Can take part in management activities, evaluate the results obtained to improve quality | ||||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 50 | |
| Rate of Final Exam to Success | 50 | |
| Total | 100 | |