The aim of this course is to know the stages of light microscope tissue processing, to have information about fixation and fixatives, to know sectioning methods, to define basic histological stains, to define metachromasia and to analyze the microscopic images obtained.
Course Content
This course contains; Light microscope tissue processing.,Fixation and fixatives,Decalcification,Sectioning methods,Practice (Tissue processing, sectioning),Basic histological stains,Midterm,Bone and muscle tissue dyes,Nerve tissue dyes,Metachromasia and metachromatic stains,Practice,Obtaining images under a light microscope,Practice (finding and analyzing images in a light microscope),Final exam.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Explains the measurement and counting methods to be used in light microscopic analyses.
14, 9
A, E
Explains light microscope tissue processing and its stages.
14, 17, 9
A, E
Determines the purposes of fixation.
14, 9
A, E
Evaluates by applying commonly used fixatives.
14, 17, 9
A
Explains the dyes used in histology by applying them.
14, 17, 9
A, E
Analyzes the image under a light microscope.
14, 17, 9
A, E
Explains sectioning methods.
14, 17, 9
A, E
Explains the concept of metachromasia and the dyes that show metachromasia.
14, 9
A
Defines the concept of decalcification.
14, 9
A
Teaching Methods:
14: Self Study Method, 17: Experimental Technique, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, E: Homework
Course Outline
Order
Subjects
Preliminary Work
1
Light microscope tissue processing.
Reading the relevant course presentation
2
Fixation and fixatives
Reading the relevant course presentation
3
Decalcification
Reading the relevant course presentation
4
Sectioning methods
Reading the relevant course presentation
5
Practice (Tissue processing, sectioning)
Ramazan Demir Histolojik Boyama Teknikleri 1st Edition Page: 22-40
6
Basic histological stains
Reading the relevant course presentation
7
Midterm
-
8
Bone and muscle tissue dyes
Reading the relevant course presentation
9
Nerve tissue dyes
Reading the relevant course presentation
10
Metachromasia and metachromatic stains
Reading the relevant course presentation
11
Practice
-
12
Obtaining images under a light microscope
Reading the relevant course presentation
13
Practice (finding and analyzing images in a light microscope)
-
14
Final exam
-
Resources
Lecture notes, Bancroft's Theory and Practice of Histological Techniques (Editors:Kim S Suvarna, Christopher Layton, John D. Bancroft), Histolojik Boyama Teknikleri (Prof. Dr. Ramazan Demir)
Course Contribution to Program Qualifications
Course Contribution to Program Qualifications
No
Program Qualification
Contribution Level
1
2
3
4
5
1
Know about histology of basic tissue, basic embryological development, mechanisms of routine micro-preparation techniques, and the basics of active use of a microscope
X
2
Have knowledge of ethics of research and the rules of documenting a research
X
3
Can relay information about basic histology-embryology
X
4
Can work on tissue preservation, tissue dye and do microscopic examination.
X
5
Can do literature review
X
6
Can attend both clinical and nonclinical studies and make proposals for methods.
X
7
Can document outcomes.
X
8
Express themselves by using resources in Histology-Embryology and in the areas of histotechnical science, and communication techniques.
X
9
Improve their critical thinking, and can create strategies.
X
10
Behave with an ethical concern in both clinical and nonclinical preparation.
X
11
Have the awareness of business ethics and create a secure working environment, evaluate the contributors.
X
12
Exhibit scientific assets in national or international scientific meetings using foreign languages besides their mother language.
13
Promote interdisciplinary cooperation with the consciousness of social responsibility and remain true to professional secrecy, create final decleration within the framework of the quality.
Assessment Methods
Contribution Level
Absolute Evaluation
Rate of Midterm Exam to Success
50
Rate of Final Exam to Success
50
Total
100
ECTS / Workload Table
Activities
Number of
Duration(Hour)
Total Workload(Hour)
Course Hours
14
6
84
Guided Problem Solving
10
4
40
Resolution of Homework Problems and Submission as a Report
6
10
60
Term Project
2
10
20
Presentation of Project / Seminar
0
0
0
Quiz
0
0
0
Midterm Exam
1
15
15
General Exam
1
15
15
Performance Task, Maintenance Plan
0
0
0
Total Workload(Hour)
234
Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(234/30)
8
ECTS of the course: 30 hours of work is counted as 1 ECTS credit.
Detail Informations of the Course
Course Description
Course
Code
Semester
T+P (Hour)
Credit
ECTS
HISTOTECHNOLOGY-I
-
Fall Semester
2+4
4
8
Course Program
Prerequisites Courses
Recommended Elective Courses
Language of Course
Turkish
Course Level
Second Cycle (Master's Degree)
Course Type
Required
Course Coordinator
Assist.Prof. Bircan KOLBAŞI
Name of Lecturer(s)
Prof.Dr. İlknur KESKİN
Assistant(s)
Aim
The aim of this course is to know the stages of light microscope tissue processing, to have information about fixation and fixatives, to know sectioning methods, to define basic histological stains, to define metachromasia and to analyze the microscopic images obtained.
Course Content
This course contains; Light microscope tissue processing.,Fixation and fixatives,Decalcification,Sectioning methods,Practice (Tissue processing, sectioning),Basic histological stains,Midterm,Bone and muscle tissue dyes,Nerve tissue dyes,Metachromasia and metachromatic stains,Practice,Obtaining images under a light microscope,Practice (finding and analyzing images in a light microscope),Final exam.
Dersin Öğrenme Kazanımları
Teaching Methods
Assessment Methods
Explains the measurement and counting methods to be used in light microscopic analyses.
14, 9
A, E
Explains light microscope tissue processing and its stages.
14, 17, 9
A, E
Determines the purposes of fixation.
14, 9
A, E
Evaluates by applying commonly used fixatives.
14, 17, 9
A
Explains the dyes used in histology by applying them.
14, 17, 9
A, E
Analyzes the image under a light microscope.
14, 17, 9
A, E
Explains sectioning methods.
14, 17, 9
A, E
Explains the concept of metachromasia and the dyes that show metachromasia.
14, 9
A
Defines the concept of decalcification.
14, 9
A
Teaching Methods:
14: Self Study Method, 17: Experimental Technique, 9: Lecture Method
Assessment Methods:
A: Traditional Written Exam, E: Homework
Course Outline
Order
Subjects
Preliminary Work
1
Light microscope tissue processing.
Reading the relevant course presentation
2
Fixation and fixatives
Reading the relevant course presentation
3
Decalcification
Reading the relevant course presentation
4
Sectioning methods
Reading the relevant course presentation
5
Practice (Tissue processing, sectioning)
Ramazan Demir Histolojik Boyama Teknikleri 1st Edition Page: 22-40
6
Basic histological stains
Reading the relevant course presentation
7
Midterm
-
8
Bone and muscle tissue dyes
Reading the relevant course presentation
9
Nerve tissue dyes
Reading the relevant course presentation
10
Metachromasia and metachromatic stains
Reading the relevant course presentation
11
Practice
-
12
Obtaining images under a light microscope
Reading the relevant course presentation
13
Practice (finding and analyzing images in a light microscope)
-
14
Final exam
-
Resources
Lecture notes, Bancroft's Theory and Practice of Histological Techniques (Editors:Kim S Suvarna, Christopher Layton, John D. Bancroft), Histolojik Boyama Teknikleri (Prof. Dr. Ramazan Demir)
Course Contribution to Program Qualifications
Course Contribution to Program Qualifications
No
Program Qualification
Contribution Level
1
2
3
4
5
1
Know about histology of basic tissue, basic embryological development, mechanisms of routine micro-preparation techniques, and the basics of active use of a microscope
X
2
Have knowledge of ethics of research and the rules of documenting a research
X
3
Can relay information about basic histology-embryology
X
4
Can work on tissue preservation, tissue dye and do microscopic examination.
X
5
Can do literature review
X
6
Can attend both clinical and nonclinical studies and make proposals for methods.
X
7
Can document outcomes.
X
8
Express themselves by using resources in Histology-Embryology and in the areas of histotechnical science, and communication techniques.
X
9
Improve their critical thinking, and can create strategies.
X
10
Behave with an ethical concern in both clinical and nonclinical preparation.
X
11
Have the awareness of business ethics and create a secure working environment, evaluate the contributors.
X
12
Exhibit scientific assets in national or international scientific meetings using foreign languages besides their mother language.
13
Promote interdisciplinary cooperation with the consciousness of social responsibility and remain true to professional secrecy, create final decleration within the framework of the quality.
