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The Proteomics laboratory is involved in the evaluation of molecular mechanisms of neurodegeneration. Advanced proteomics tools like label-free differential proteome expression analysis, PTM analysis and targeted proteomics is used to study the genesis and progression of neurological diseases like Alzheimer´s and Parkinson´s diseases.

MALDI SYNAPT G2-Si MS System enables production optimal results for imaging, chemical materials characterization, proteomics and pharmaceutical applications. The MALDI SYNAPT G2-Si is powered by a solid state laser with a repetition rate of 2.5KHz to maximize the speed of analysis. The laser spot size can be selected for optimising sensitivity and speed of analysis, or for maximizing spatial resolution in Imaging studies. Due to the orthogonal geometry of the Tof analyzer, the source is decoupled from the mass analysis. So, unlike axial MALDI-Tof or Tof/Tof instruments, this ensures that high resolution and accurate mass are achieved in both MS and MS/MS modes across a wide mass range. In addition, SYNAPT is well suited to nonconductive samples such as paraffin embedded tissue sections on glass slides.

High Definition Imaging (HDI) MALDI workflows

MALDI SYNAPT G2-Si HDMS combines T-Wave IMS and QuanTof technology within a streamlined imaging workflow to deliver unparalleled selectivity, clarity and confidence. The HDI MALDI solution provides a unique range of powerful multiplex targeted (IMS/MS/MS) and untargeted (IMS/MSE) workflows from image-centric method set-up to data processing and image generation. Integrated correlation (based on spatial location drift-time related fragment ions) and statistical tools (e.g. PCA, OPLS-DA, S-plots, hierarchical clustering) also combine to deliver more intelligent and confident imaging analysis. The ability to access full UPLC/MS/MS capability on SYNAPT delivers the power of high performance quantitative and discovery analysis from bio-fluids or laser-dissected tissue sections on the same platform.

Application Areas:

  • Proteomics
  • MALDI Imaging

ACQUITY QDa is as intuitive as an optical detector, with the robustness to handle analyses. Working in harmony with your chromatography, it is pre-optimized to work with small molecule samples, without the sample-specific or user adjustments. Analytical scientist can consistently generate the high quality mass spectral data, routinely without the need for any special training or expertise. The QDa detector is coupled to an Acquity UPLC system and provides mass information to every chromatographically seperated peak.

Application Areas:

  • Monitoring reactions intermediates and products.
  • Drug impurity analysis
  • Screening small molecule compound in biological samples.

The Waters 2998 Photodiode Array (PDA) Detector offers advanced optical detection, providing unprecedented trace impurity detection and quantitation in conjunction with spectral analysis capabilities. It is the ideal detector from compound identification to method development.

Integrated solvent and sample management to ensure consistent system-to-system performance and high reproducibility
Full integration with Empower Software for instrument control and data processing
A large, intuitive LCD-based user interface allows rapid system set-up through SystemPREP routines that streamline daily start-ups

Designed for the most demanding quantitative UPLC/MS/MS applications. The ultimate in tandem quadrupole performance allows unrivalled sensitivity and robustness.

  • Class leading sensitivity and robustness.
  • Qualitative information
  • Accelerated method development for sensitive and robust methods.
  • The wide range of ionization capabilities

Class leading sensitivity and robustness

The innovative StepWave ion guide, delivers the highest levels of sensitivity and robustness. The Xevo TQ-S enables the lowest detection limits to be achieved for the most challenging analyses, whilst maintaining instrument up time, due to the innovative active separation of ions from neutrals.

Qualitative information when you need it most

With its unique collision cell, Xevo TQ-S enables you to better address your analytical needs with the option to operate in ScanWave enhanced product ion scanning mode. This mode enables you to more easily confirm the identities and structures of your analytes of interest, at the low concentrations required

Application Areas:

  • Targeted Proteomics
  • Metabolite quantitation in biological samples
  • Drug impurity analysis
  • Pesticide screening
  • Trace molecule analysis

Expand the dynamic range of your analysis with 2D separations.

  • The ACQUITY UPLC® M-Class System with 2D Technology expands the use of sub-2-micron particles to achieve high peak capacity separations. This innovative system effectively uses two-dimensional (2D) UPLC for better chromatographic resolution of complex proteomic samples by using a dual reversed-phase (RP) approach.
  • High-resolution in both dimensions by exploiting the wide-ranging ionic and hydrophobic structure of peptides
  • Better protein identifications, quantification, and sequence coverage
  • Improved separation, method generation wizards, and enhanced data algorithms

Comprehensive 2D – Expanding the impact.

Conventional 2D-LC uses ion exchange (IEX) followed by reversed- phase (RP). Any IEX approach will use salt-containing buffers that can cause ionization background and fouling problems if they enter a mass spectrometer (MS). Since IEX separations are based solely on the charge of the peptide, the IEX dimension often results in poor chromatographic resolution with peptides appearing in multiple fractions, making data interpretation difficult.

This improved 2D approach uses RP at pH 10 in the first dimension, followed by RP at pH 2 in the second dimension for results that far exceeds those of conventional IEX methodologies.

Application Areas:

  • Deeper proteome coverage
  • Trace impurity analysis

The ACQUITY UPLC® I-Class System provides the most powerful solution to the most critical need in separation science today – successfully analyzing compounds that are limited in amount or availability amid a complex matrix, more rapidly than ever before. Developed to produce the most accurate and reproducible separations. Complex separation challenges require LC systems designed to maximize the benefits of sub- 2-µm particle columns integrated in a system designed to optimize MS performance.

  • Maximizes peak capacity to enhance MS sensitivity
  • Provides the lowest carryover, complementing MS sensitivity and extending MS linear dynamic range
  • Has been purposefully engineered for the lowest dispersion; with an extended pressure/flow envelope, complex separations can be accelerated without compromising chromatographic fidelity

Application Areas:

  • Metabolomics
  • Lipidomics
  • Biotherapeutics analysis
  • Trace analysis

SYNAPT provides the most complete characterization of complex mixtures and molecules with unique levels of MS performance, industry leading informatics and unparalleled platform versatility.

  • Superior separation with high-efficiency T-Wave IMS
  • Ultimate UPLC/MS/MS performance
  • Data independent & data dependant T-Wave IMS solutions
  • CID and ETD fragmentation capabilities
  • Wide range of experimental options

Superior separation with high-efficiency T-Wave IMS

Retention. Mobility. Mass. Chromatography and mass resolution are not enough. With high-efficiency T-Wave ion mobility, there is an additional dimension of separation, based on molecular size and shape. The unique ability to use the collision cross section property of a molecule delivers the highest levels of selectivity, specificity, sensitivity and structural insight. The wide range of benefits feature isomer separation, elimination of interferences, generation of cleaner spectra and even the ability to better confirm compound ID.

CID and ETD Fragmentation Capabilities

The dual collision cell arrangement of TriWave enhances MS/MS possibilities, by providing Collision Induced Dissociation (CID) and/or Electron Transfer Dissociation (ETD) fragmentation with high resolution and accurate mass measurements.

Application Areas:

  • Metabolomics
  • Lipidomics
  • Protein Expression analysis
  • PTM analysis
  • Intact mas analysis
  • Biopharmaceutical drug analysis