Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| TOXICOLOGY LAB. | PHA4214877 | Spring Semester | 0+3 | 1,5 | 3 |
| Course Program | ( B ) Çarşamba 13:30-14:15 ( B ) Çarşamba 14:30-15:15 ( B ) Çarşamba 15:30-16:15 ( A ) Cuma 13:30-14:15 ( A ) Cuma 14:30-15:15 ( A ) Cuma 15:30-16:15 |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | English |
| Course Level | First Cycle (Bachelor's Degree) |
| Course Type | Required |
| Course Coordinator | Prof.Dr. Gülden Zehra OMURTAG |
| Name of Lecturer(s) | Prof.Dr. Gülden Zehra OMURTAG |
| Assistant(s) | Res. assist. Meryem Nur Baş, Res. assist Özce Esma PALA |
| Aim | The aim of this course is to give information about systematic toxicology, the determination of metallic, natural, and synthetic poisons in biological materials using chemical and instrumental methods and to perform their applications. |
| Course Content | This course contains; In vitro cytotoxicity tests,Genotoxicity techniques, basic cell culture,Acute toxicity (LD50) determination,Varıous techniques used in screening methods of poisons,Determination of poisons in biological materials and their isolation techniques,Analysis of toxic compounds in biological materials: Volatile poisons,Analysis of toxic compounds in biological materials: Nonvolatile poisons,Polymerase chain reaction (PCR),Quantitation of salicylate and paracetamol - Interpretation of DONE nomogram,Determination of volatile substances in biological samples by microdifusion techniques,Determination of non volatile metallic poisons in biological samples,Qualitative determination of acidic and basic drugs by chromatographic method,Methemoglobin quantification and cyanide determination in blood,Isolation of DNA from blood. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| 1. Explains the general tests used in cell studies and their principles. | 17, 5, 9 | A, D |
| 1.1. Explains in vitro cytotoxicity tests and the principles on which they are based. | 17, 5, 9 | A, D |
| 1.2. Explains in vitro genotoxicity tests and the principles on which they are based. | 17, 5, 9 | A, D |
| 1.3. Explains the polymerase chain reaction (PCR), the basis of the reaction, its stages and its areas of use in toxicology. | 17, 5, 9 | A, D |
| 2. Performs quantitative and qualitative determinations of xenobiotics from biological materials. | 17, 5, 9 | A, D |
| 2.1. Analyzes volatile poisons in biological material. | 17, 5, 9 | A, D |
| 2.2. Performs qualitative and quantitative analyses of salicylate and paracetamol. | 17, 5, 9 | A, D |
| 2.3. Analyzes important non-volatile poisons in biological material. | 17, 5, 9 | A, D |
| 3. Defines the concepts of acute toxicity and LD50. | 17, 5, 9 | A, D |
| 4. Explains the devices and principles used in poison screening methods in the context of toxicology. | 17, 5, 9 | A, D |
| 4.1. Determines acetic and basic drugs qualitatively by chromatographic method. | 17, 5, 9 | A, D |
| 5 Explains the methods of direct detection and isolation of poisons in biological material. | 17, 5, 9 | A, D |
| 5.1. Determines the amount of methemoglobin in the blood and the presence of cyanide in the blood. | 17, 5, 9 | A, D |
| 5.2. Isolates DNA from blood and explains its principles. | 17, 5, 9 | A, D |
| 5.3. Explains the principles of microdiffusion technique and determines volatile substances in biological samples using microdiffusion technique. | 17, 5, 9 | A, D |
| Teaching Methods: | 17: Experimental Technique, 5: Cooperative Learning, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, D: Oral Exam |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | In vitro cytotoxicity tests | 1,2 |
| 2 | Genotoxicity techniques, basic cell culture | 1,2 |
| 3 | Acute toxicity (LD50) determination | 1,2 |
| 4 | Varıous techniques used in screening methods of poisons | 1,2 |
| 5 | Determination of poisons in biological materials and their isolation techniques | 1,2 |
| 6 | Analysis of toxic compounds in biological materials: Volatile poisons | 1,2 |
| 7 | Analysis of toxic compounds in biological materials: Nonvolatile poisons | 1,2 |
| 8 | Polymerase chain reaction (PCR) | 1,2 |
| 9 | Quantitation of salicylate and paracetamol - Interpretation of DONE nomogram | 1,2 |
| 10 | Determination of volatile substances in biological samples by microdifusion techniques | 1,2 |
| 11 | Determination of non volatile metallic poisons in biological samples | 1,2 |
| 12 | Qualitative determination of acidic and basic drugs by chromatographic method | 1,2 |
| 13 | Methemoglobin quantification and cyanide determination in blood | 1,2 |
| 14 | Isolation of DNA from blood | 1,2 |
| Resources |
| 1. The laboratory notes. 2. Demonstration |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | PQ-1. Skilfully use information technology to access, follow, evaluate and apply current knowledge in the field of Pharmaceutical Sciences. | ||||||
| 2 | PQ-2. Have advanced knowledge in APIs and DPs. | ||||||
| 3 | PQ-3. Collaborate with the experts for collection, interpretation, application and declaration of data in Pharmaceutical Sciences field. | ||||||
| 4 | PQ-4. Provide best patient counseling services and medication therapy management using his/her knowledge and pharmaceutical information systems to improve the quality of life and safety of patient. | X | |||||
| 5 | PQ-5. Capable of organizing and administering a drugstore as per responsibilities prescribed under the Pharmacy Law, using skills, knowledge and advanced technology systems. | ||||||
| 6 | PQ-6. Skilfully use computer programs and advanced technology related to the Pharmaceutical Sciences. | X | |||||
| 7 | PQ-7. Provide solutions to the professional issues using scientific knowledge and data. | ||||||
| 8 | PQ-8. Perform identification, quality control and standardization of the drug products. | ||||||
| 9 | PQ-9. Prepare and implement developmental plans for his/her subordinates, frequently evaluate their progress and make necessary adjustments. | ||||||
| 10 | PQ-10. Capable of conducting independent studies with his/her advanced knowledge in pharmaceutical sciences, takes responsibilities in professional organizations and efficiently collaborates with the members of his/her own organization as well as other organizations. | X | |||||
| 11 | PQ-11. Clearly show signs of adoption of life-long learning and openness to further development. | ||||||
| 12 | PQ-12. Critically evaluate their knowledge in pharmaceutical sciences. | ||||||
| 13 | PQ-13. Closely follow and evaluate the national and international professional developments. | ||||||
| 14 | PQ-14. Record and document all professional activities and applications. | ||||||
| 15 | PQ-15. Communicate all necessary information about medicines to patient, medical personnel and public. | X | |||||
| 16 | PQ-16. Proficient in English to communicate and follow current knowledge in pharmaceutical sciences. | ||||||
| 17 | PQ-17. Inform officials and organizations about the issues with the quality assurance of pharmaceutical products. | X | |||||
| 18 | PQ-18. Actively involve in the preparation of APIs, every stage of the formulation and production of DPs. | ||||||
| 19 | PQ-19. Knowledgable in the regulatory rules of new drug application, data protection and patent issues and understand their interrelationship. | ||||||
| 20 | PQ-20. Have awareness of his/her duties, rights and responsibilities and act based on Pharmacy Law and high professional ethical standards. | ||||||
| 21 | PQ-21. Familiar with and actively pursue the personal and public health, environmental, and work safety issues. | X | |||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 60 | |
| Rate of Final Exam to Success | 40 | |
| Total | 100 | |
| ECTS / Workload Table | ||||||
| Activities | Number of | Duration(Hour) | Total Workload(Hour) | |||
| Course Hours | 14 | 3 | 42 | |||
| Guided Problem Solving | 0 | 0 | 0 | |||
| Resolution of Homework Problems and Submission as a Report | 14 | 2 | 28 | |||
| Term Project | 0 | 0 | 0 | |||
| Presentation of Project / Seminar | 0 | 0 | 0 | |||
| Quiz | 1 | 4 | 4 | |||
| Midterm Exam | 1 | 6 | 6 | |||
| General Exam | 1 | 11 | 11 | |||
| Performance Task, Maintenance Plan | 0 | 0 | 0 | |||
| Total Workload(Hour) | 91 | |||||
| Dersin AKTS Kredisi = Toplam İş Yükü (Saat)/30*=(91/30) | 3 | |||||
| ECTS of the course: 30 hours of work is counted as 1 ECTS credit. | ||||||
Detail Informations of the Course
Course Description
| Course | Code | Semester | T+P (Hour) | Credit | ECTS |
|---|---|---|---|---|---|
| TOXICOLOGY LAB. | PHA4214877 | Spring Semester | 0+3 | 1,5 | 3 |
| Course Program | ( B ) Çarşamba 13:30-14:15 ( B ) Çarşamba 14:30-15:15 ( B ) Çarşamba 15:30-16:15 ( A ) Cuma 13:30-14:15 ( A ) Cuma 14:30-15:15 ( A ) Cuma 15:30-16:15 |
| Prerequisites Courses | |
| Recommended Elective Courses |
| Language of Course | English |
| Course Level | First Cycle (Bachelor's Degree) |
| Course Type | Required |
| Course Coordinator | Prof.Dr. Gülden Zehra OMURTAG |
| Name of Lecturer(s) | Prof.Dr. Gülden Zehra OMURTAG |
| Assistant(s) | Res. assist. Meryem Nur Baş, Res. assist Özce Esma PALA |
| Aim | The aim of this course is to give information about systematic toxicology, the determination of metallic, natural, and synthetic poisons in biological materials using chemical and instrumental methods and to perform their applications. |
| Course Content | This course contains; In vitro cytotoxicity tests,Genotoxicity techniques, basic cell culture,Acute toxicity (LD50) determination,Varıous techniques used in screening methods of poisons,Determination of poisons in biological materials and their isolation techniques,Analysis of toxic compounds in biological materials: Volatile poisons,Analysis of toxic compounds in biological materials: Nonvolatile poisons,Polymerase chain reaction (PCR),Quantitation of salicylate and paracetamol - Interpretation of DONE nomogram,Determination of volatile substances in biological samples by microdifusion techniques,Determination of non volatile metallic poisons in biological samples,Qualitative determination of acidic and basic drugs by chromatographic method,Methemoglobin quantification and cyanide determination in blood,Isolation of DNA from blood. |
| Dersin Öğrenme Kazanımları | Teaching Methods | Assessment Methods |
| 1. Explains the general tests used in cell studies and their principles. | 17, 5, 9 | A, D |
| 1.1. Explains in vitro cytotoxicity tests and the principles on which they are based. | 17, 5, 9 | A, D |
| 1.2. Explains in vitro genotoxicity tests and the principles on which they are based. | 17, 5, 9 | A, D |
| 1.3. Explains the polymerase chain reaction (PCR), the basis of the reaction, its stages and its areas of use in toxicology. | 17, 5, 9 | A, D |
| 2. Performs quantitative and qualitative determinations of xenobiotics from biological materials. | 17, 5, 9 | A, D |
| 2.1. Analyzes volatile poisons in biological material. | 17, 5, 9 | A, D |
| 2.2. Performs qualitative and quantitative analyses of salicylate and paracetamol. | 17, 5, 9 | A, D |
| 2.3. Analyzes important non-volatile poisons in biological material. | 17, 5, 9 | A, D |
| 3. Defines the concepts of acute toxicity and LD50. | 17, 5, 9 | A, D |
| 4. Explains the devices and principles used in poison screening methods in the context of toxicology. | 17, 5, 9 | A, D |
| 4.1. Determines acetic and basic drugs qualitatively by chromatographic method. | 17, 5, 9 | A, D |
| 5 Explains the methods of direct detection and isolation of poisons in biological material. | 17, 5, 9 | A, D |
| 5.1. Determines the amount of methemoglobin in the blood and the presence of cyanide in the blood. | 17, 5, 9 | A, D |
| 5.2. Isolates DNA from blood and explains its principles. | 17, 5, 9 | A, D |
| 5.3. Explains the principles of microdiffusion technique and determines volatile substances in biological samples using microdiffusion technique. | 17, 5, 9 | A, D |
| Teaching Methods: | 17: Experimental Technique, 5: Cooperative Learning, 9: Lecture Method |
| Assessment Methods: | A: Traditional Written Exam, D: Oral Exam |
Course Outline
| Order | Subjects | Preliminary Work |
|---|---|---|
| 1 | In vitro cytotoxicity tests | 1,2 |
| 2 | Genotoxicity techniques, basic cell culture | 1,2 |
| 3 | Acute toxicity (LD50) determination | 1,2 |
| 4 | Varıous techniques used in screening methods of poisons | 1,2 |
| 5 | Determination of poisons in biological materials and their isolation techniques | 1,2 |
| 6 | Analysis of toxic compounds in biological materials: Volatile poisons | 1,2 |
| 7 | Analysis of toxic compounds in biological materials: Nonvolatile poisons | 1,2 |
| 8 | Polymerase chain reaction (PCR) | 1,2 |
| 9 | Quantitation of salicylate and paracetamol - Interpretation of DONE nomogram | 1,2 |
| 10 | Determination of volatile substances in biological samples by microdifusion techniques | 1,2 |
| 11 | Determination of non volatile metallic poisons in biological samples | 1,2 |
| 12 | Qualitative determination of acidic and basic drugs by chromatographic method | 1,2 |
| 13 | Methemoglobin quantification and cyanide determination in blood | 1,2 |
| 14 | Isolation of DNA from blood | 1,2 |
| Resources |
| 1. The laboratory notes. 2. Demonstration |
Course Contribution to Program Qualifications
| Course Contribution to Program Qualifications | |||||||
| No | Program Qualification | Contribution Level | |||||
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | PQ-1. Skilfully use information technology to access, follow, evaluate and apply current knowledge in the field of Pharmaceutical Sciences. | ||||||
| 2 | PQ-2. Have advanced knowledge in APIs and DPs. | ||||||
| 3 | PQ-3. Collaborate with the experts for collection, interpretation, application and declaration of data in Pharmaceutical Sciences field. | ||||||
| 4 | PQ-4. Provide best patient counseling services and medication therapy management using his/her knowledge and pharmaceutical information systems to improve the quality of life and safety of patient. | X | |||||
| 5 | PQ-5. Capable of organizing and administering a drugstore as per responsibilities prescribed under the Pharmacy Law, using skills, knowledge and advanced technology systems. | ||||||
| 6 | PQ-6. Skilfully use computer programs and advanced technology related to the Pharmaceutical Sciences. | X | |||||
| 7 | PQ-7. Provide solutions to the professional issues using scientific knowledge and data. | ||||||
| 8 | PQ-8. Perform identification, quality control and standardization of the drug products. | ||||||
| 9 | PQ-9. Prepare and implement developmental plans for his/her subordinates, frequently evaluate their progress and make necessary adjustments. | ||||||
| 10 | PQ-10. Capable of conducting independent studies with his/her advanced knowledge in pharmaceutical sciences, takes responsibilities in professional organizations and efficiently collaborates with the members of his/her own organization as well as other organizations. | X | |||||
| 11 | PQ-11. Clearly show signs of adoption of life-long learning and openness to further development. | ||||||
| 12 | PQ-12. Critically evaluate their knowledge in pharmaceutical sciences. | ||||||
| 13 | PQ-13. Closely follow and evaluate the national and international professional developments. | ||||||
| 14 | PQ-14. Record and document all professional activities and applications. | ||||||
| 15 | PQ-15. Communicate all necessary information about medicines to patient, medical personnel and public. | X | |||||
| 16 | PQ-16. Proficient in English to communicate and follow current knowledge in pharmaceutical sciences. | ||||||
| 17 | PQ-17. Inform officials and organizations about the issues with the quality assurance of pharmaceutical products. | X | |||||
| 18 | PQ-18. Actively involve in the preparation of APIs, every stage of the formulation and production of DPs. | ||||||
| 19 | PQ-19. Knowledgable in the regulatory rules of new drug application, data protection and patent issues and understand their interrelationship. | ||||||
| 20 | PQ-20. Have awareness of his/her duties, rights and responsibilities and act based on Pharmacy Law and high professional ethical standards. | ||||||
| 21 | PQ-21. Familiar with and actively pursue the personal and public health, environmental, and work safety issues. | X | |||||
Assessment Methods
| Contribution Level | Absolute Evaluation | |
| Rate of Midterm Exam to Success | 60 | |
| Rate of Final Exam to Success | 40 | |
| Total | 100 | |